Human Gene KCNK16 (ENST00000373229.9) from GENCODE V44
  Description: Homo sapiens potassium two pore domain channel subfamily K member 16 (KCNK16), transcript variant 2, mRNA. (from RefSeq NM_032115)
RefSeq Summary (NM_032115): The protein encoded by this gene belongs to the family of potassium channel proteins containing two pore-forming P domains. This channel is an open rectifier which primarily passes outward current under physiological K+ concentrations. This gene is expressed predominantly in the pancreas and is activated at alkaline pH. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Sep 2008].
Gencode Transcript: ENST00000373229.9
Gencode Gene: ENSG00000095981.11
Transcript (Including UTRs)
   Position: hg38 chr6:39,314,698-39,322,554 Size: 7,857 Total Exon Count: 6 Strand: -
Coding Region
   Position: hg38 chr6:39,315,002-39,322,540 Size: 7,539 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr6:39,314,698-39,322,554)mRNA (may differ from genome)Protein (309 aa)
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HGNCLynxMalacardsMGIneXtProtOMIM
PubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: KCNKG_HUMAN
DESCRIPTION: RecName: Full=Potassium channel subfamily K member 16; AltName: Full=2P domain potassium channel Talk-1; AltName: Full=TWIK-related alkaline pH-activated K(+) channel 1; Short=TALK-1;
FUNCTION: Outward rectifying potassium channel. Produces rapidly activating and non-inactivating outward rectifier K(+) currents.
SUBUNIT: Homodimer (Potential).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Highly expressed in pancreas. Not detectable in the other tissues tested.
MISCELLANEOUS: Inhibited by Ba(2+), quinine, quinidine, chloroform and halothane. Activated at alkaline pH.
SIMILARITY: Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 1.47 RPKM in Pancreas
Total median expression: 1.84 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
3' UTR -102.10304-0.336 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR003280 - 2pore_dom_K_chnl
IPR003092 - 2pore_dom_K_chnl_TASK
IPR013099 - Ion_trans_2

Pfam Domains:
PF07885 - Ion channel

ModBase Predicted Comparative 3D Structure on Q96T55
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005244 voltage-gated ion channel activity
GO:0005267 potassium channel activity
GO:0005515 protein binding
GO:0022841 potassium ion leak channel activity

Biological Process:
GO:0006811 ion transport
GO:0006813 potassium ion transport
GO:0030322 stabilization of membrane potential
GO:0034765 regulation of ion transmembrane transport
GO:0071805 potassium ion transmembrane transport

Cellular Component:
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  BC111860 - Homo sapiens potassium channel, subfamily K, member 16, mRNA (cDNA clone MGC:133123 IMAGE:40016308), complete cds.
AF358909 - Homo sapiens 2P domain potassium channel Talk-1 (KCNK16) mRNA, complete cds.
AY253147 - Homo sapiens pancreatic potassium channel TALK-1d mRNA, complete cds; alternatively spliced.
AY253146 - Homo sapiens pancreatic potassium channel TALK-1c mRNA, complete cds; alternatively spliced.
KJ894905 - Synthetic construct Homo sapiens clone ccsbBroadEn_04299 KCNK16 gene, encodes complete protein.
EU978943 - Homo sapiens K2P16.1 potassium channel (KCNK16) mRNA, complete cds.
AY253145 - Homo sapiens pancreatic potassium channel TALK-1b mRNA, complete cds; alternatively spliced.
JD508309 - Sequence 489333 from Patent EP1572962.
JD485956 - Sequence 466980 from Patent EP1572962.
JD219607 - Sequence 200631 from Patent EP1572962.
JD150652 - Sequence 131676 from Patent EP1572962.
JD340588 - Sequence 321612 from Patent EP1572962.
JD402652 - Sequence 383676 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q96T55 (Reactome details) participates in the following event(s):

R-HSA-1299359 Activation of TALK
R-HSA-5578910 KCNK dimers transport K+ from cytosol to extracellular region
R-HSA-1299361 TWIK-related alkaline pH activated K+ channel (TALK)
R-HSA-5576886 Phase 4 - resting membrane potential
R-HSA-1296346 Tandem pore domain potassium channels
R-HSA-5576891 Cardiac conduction
R-HSA-1296071 Potassium Channels
R-HSA-397014 Muscle contraction
R-HSA-112316 Neuronal System

-  Other Names for This Gene
  Alternate Gene Symbols: B5TJL9, ENST00000373229.1, ENST00000373229.2, ENST00000373229.3, ENST00000373229.4, ENST00000373229.5, ENST00000373229.6, ENST00000373229.7, ENST00000373229.8, KCNKG_HUMAN, NM_032115, Q2M2N9, Q5TCF3, Q6X6Z3, Q6X6Z4, Q6X6Z5, Q96T55, Q9H591, TALK1, uc003ooq.1, uc003ooq.2, uc003ooq.3
UCSC ID: ENST00000373229.9
RefSeq Accession: NM_032115
Protein: Q96T55 (aka KCNKG_HUMAN or CIWG_HUMAN)
CCDS: CCDS4843.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.