Human Gene DYNLT2 (ENST00000366774.4) from GENCODE V44
  Description: Homo sapiens t-complex-associated-testis-expressed 3 (TCTE3), transcript variant 1, mRNA. (from RefSeq NM_174910)
Gencode Transcript: ENST00000366774.4
Gencode Gene: ENSG00000184786.6
Transcript (Including UTRs)
   Position: hg38 chr6:169,740,109-169,751,572 Size: 11,464 Total Exon Count: 4 Strand: -
Coding Region
   Position: hg38 chr6:169,740,185-169,751,458 Size: 11,274 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersRNA-Seq ExpressionMicroarray Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathways
Other NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr6:169,740,109-169,751,572)mRNA (may differ from genome)Protein (198 aa)
Gene SorterGenome BrowserOther Species FASTATable SchemaAlphaFoldBioGPS
EnsemblEntrez GeneExonPrimerGencodeGeneCardsHPRD
LynxMalacardsMGIneXtProtOMIMPubMed
ReactomeUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TC1D3_HUMAN
DESCRIPTION: RecName: Full=Tctex1 domain-containing protein 3; AltName: Full=T-complex testis-specific protein 3; AltName: Full=T-complex-associated testis-expressed protein 3; Short=Tcte-3;
FUNCTION: May be an accessory component of axonemal dynein and cytoplasmic dynein 1. Candidate for involvement in male sterility (By similarity).
SUBUNIT: Interacts with CSNK2B (By similarity).
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton (Probable). Cytoplasmic granule (By similarity). Membrane; Peripheral membrane protein (By similarity). Note=Found on the surface of sperm tail. Stored in cytoplasmic granules during spermatogenesis (By similarity).
TISSUE SPECIFICITY: Expressed predominantly in testis. Also expressed in brain, lung and trachea.
SIMILARITY: Belongs to the dynein light chain Tctex-type family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 17.74 RPKM in Testis
Total median expression: 34.15 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -45.10114-0.396 Picture PostScript Text
3' UTR -8.4076-0.111 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR005334 - Tctex

Pfam Domains:
PF03645 - Tctex-1 family

ModBase Predicted Comparative 3D Structure on Q8IZS6
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003774 motor activity

Cellular Component:
GO:0005737 cytoplasm
GO:0005856 cytoskeleton
GO:0005874 microtubule
GO:0016020 membrane
GO:0030286 dynein complex


-  Descriptions from all associated GenBank mRNAs
  AF519569 - Homo sapiens dynein light chain 2 TCTEX2 mRNA, complete cds.
BC113639 - Homo sapiens t-complex-associated-testis-expressed 3, mRNA (cDNA clone MGC:142199 IMAGE:8322691), complete cds.
JD318511 - Sequence 299535 from Patent EP1572962.
KJ892262 - Synthetic construct Homo sapiens clone ccsbBroadEn_01656 TCTE3 gene, encodes complete protein.
HQ258741 - Synthetic construct Homo sapiens clone IMAGE:100072771 t-complex-associated-testis-expressed 3 (TCTE3) gene, encodes complete protein.
JD502592 - Sequence 483616 from Patent EP1572962.
BC043594 - Homo sapiens, clone IMAGE:5760980, mRNA.
BC063455 - Homo sapiens cDNA clone IMAGE:5168720, partial cds.
BC053352 - Homo sapiens cDNA clone IMAGE:5245054, partial cds.
AK023627 - Homo sapiens cDNA FLJ13565 fis, clone PLACE1008231.
JD498552 - Sequence 479576 from Patent EP1572962.
JD495083 - Sequence 476107 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q8IZS6 (Reactome details) participates in the following event(s):

R-HSA-5624949 Assembly of the anterograde IFT train
R-HSA-5625424 The retrograde IFT train dissociates
R-HSA-5625421 The anterograde IFT train dissociates
R-HSA-5624952 Assembly of the retrograde IFT train
R-HSA-5620924 Intraflagellar transport
R-HSA-5617833 Cilium Assembly
R-HSA-1852241 Organelle biogenesis and maintenance

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000366774.1, ENST00000366774.2, ENST00000366774.3, NM_174910, Q8IZS6, TC1D3_HUMAN, TCTE3, TCTEX1D3, uc003qxe.1, uc003qxe.2, uc003qxe.3
UCSC ID: ENST00000366774.4
RefSeq Accession: NM_174910
Protein: Q8IZS6 (aka TC1D3_HUMAN)
CCDS: CCDS5310.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.