Human Gene MRPL32 (ENST00000223324.3) from GENCODE V44
Description: Homo sapiens mitochondrial ribosomal protein L32 (MRPL32), transcript variant 2, non-coding RNA. (from RefSeq NR_156497) RefSeq Summary (NM_031903): Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp. [provided by RefSeq, Jul 2008]. Gencode Transcript: ENST00000223324.3 Gencode Gene: ENSG00000106591.4 Transcript (Including UTRs) Position: hg38 chr7:42,932,376-42,937,857 Size: 5,482 Total Exon Count: 3 Strand: + Coding Region Position: hg38 chr7:42,932,387-42,937,576 Size: 5,190 Coding Exon Count: 3
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF01783 - Ribosomal L32p protein family
ModBase Predicted Comparative 3D Structure on Q9BYC8
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AB049649 - Homo sapiens MRPL32 mRNA for mitochondrial ribosomal protein L32 (L32mt), complete cds. LF384901 - JP 2014500723-A/192404: Polycomb-Associated Non-Coding RNAs. MA620478 - JP 2018138019-A/192404: Polycomb-Associated Non-Coding RNAs. JD215160 - Sequence 196184 from Patent EP1572962. AK090551 - Homo sapiens cDNA FLJ33232 fis, clone ASTRO2002024, highly similar to 39S ribosomal protein L32, mitochondrial precursor. AX746505 - Sequence 30 from Patent EP1308459. BC013147 - Homo sapiens mitochondrial ribosomal protein L32, mRNA (cDNA clone MGC:21626 IMAGE:4340082), complete cds. AK313393 - Homo sapiens cDNA, FLJ93927, Homo sapiens mitochondrial ribosomal protein L32 (MRPL32), nucleargene encoding mitochondrial protein, mRNA. CU680524 - Synthetic construct Homo sapiens gateway clone IMAGE:100020169 5' read MRPL32 mRNA. HQ448324 - Synthetic construct Homo sapiens clone IMAGE:100071742; CCSB000872_03 mitochondrial ribosomal protein L32 (MRPL32) gene, encodes complete protein. KJ899490 - Synthetic construct Homo sapiens clone ccsbBroadEn_08884 MRPL32 gene, encodes complete protein. AF161401 - Homo sapiens HSPC283 mRNA, partial cds. LF330979 - JP 2014500723-A/138482: Polycomb-Associated Non-Coding RNAs. MA566556 - JP 2018138019-A/138482: Polycomb-Associated Non-Coding RNAs. LF330977 - JP 2014500723-A/138480: Polycomb-Associated Non-Coding RNAs. MA566554 - JP 2018138019-A/138480: Polycomb-Associated Non-Coding RNAs. LF330976 - JP 2014500723-A/138479: Polycomb-Associated Non-Coding RNAs. MA566553 - JP 2018138019-A/138479: Polycomb-Associated Non-Coding RNAs.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q9BYC8 (Reactome details) participates in the following event(s):
R-HSA-5389839 39S subunit binds 28S subunit:mRNA:fMet-tRNA R-HSA-5419273 Hydrolysis of GTP and dissociation of 28S and 39S subunits R-HSA-5389848 TUFM:GTP:aminoacyl-tRNA binds 55S ribosome:mRNA:fMet-tRNA R-HSA-5389857 Peptide transfer from P-site to A-site (peptide bond formation) R-HSA-5389842 TUFM hydrolyzes GTP and TUFM:GDP dissociates from 55S ribosome R-HSA-5419261 55S ribosome with peptidyl-tRNA in A site binds GFM1:GTP R-HSA-5419281 MRRF binds 55S ribosome:mRNA:tRNA R-HSA-5419279 Translocation of peptidyl-tRNA from A-site to P-site (and translocation of 55S ribosome by 3 bases along mRNA) R-HSA-5419264 MTRF1L (MTRF1a) or ICT1 binds stop codon in 55S ribosome:mRNA:peptidyl-tRNA R-HSA-5419277 GFM2:GTP binds 55S ribosome:mRNA:tRNA:MRRF releasing mRNA and tRNA R-HSA-5368286 Mitochondrial translation initiation R-HSA-5419276 Mitochondrial translation termination R-HSA-5368287 Mitochondrial translation R-HSA-5389840 Mitochondrial translation elongation R-HSA-72766 Translation R-HSA-392499 Metabolism of proteins