Human Gene MRPL32 (ENST00000223324.3) from GENCODE V44
  Description: Homo sapiens mitochondrial ribosomal protein L32 (MRPL32), transcript variant 2, non-coding RNA. (from RefSeq NR_156497)
RefSeq Summary (NM_031903): Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp. [provided by RefSeq, Jul 2008].
Gencode Transcript: ENST00000223324.3
Gencode Gene: ENSG00000106591.4
Transcript (Including UTRs)
   Position: hg38 chr7:42,932,376-42,937,857 Size: 5,482 Total Exon Count: 3 Strand: +
Coding Region
   Position: hg38 chr7:42,932,387-42,937,576 Size: 5,190 Coding Exon Count: 3 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:42,932,376-42,937,857)mRNA (may differ from genome)Protein (188 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCLynxMGIneXtProtOMIMPubMed
ReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: RM32_HUMAN
DESCRIPTION: RecName: Full=39S ribosomal protein L32, mitochondrial; Short=L32mt; Short=MRP-L32; Flags: Precursor;
SUBCELLULAR LOCATION: Mitochondrion.
SIMILARITY: Belongs to the ribosomal protein L32P family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 19.02 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 557.14 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
3' UTR -35.20281-0.125 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002677 - Ribosomal_L32p

Pfam Domains:
PF01783 - Ribosomal L32p protein family

ModBase Predicted Comparative 3D Structure on Q9BYC8
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserGenome BrowserGenome BrowserNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsemblEnsemblWormBase 
Protein SequenceProtein SequenceProtein SequenceProtein SequenceProtein Sequence 
AlignmentAlignmentAlignmentAlignmentAlignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003723 RNA binding
GO:0003735 structural constituent of ribosome

Biological Process:
GO:0006412 translation
GO:0070125 mitochondrial translational elongation
GO:0070126 mitochondrial translational termination

Cellular Component:
GO:0005739 mitochondrion
GO:0005743 mitochondrial inner membrane
GO:0005761 mitochondrial ribosome
GO:0005762 mitochondrial large ribosomal subunit
GO:0005840 ribosome
GO:0015934 large ribosomal subunit


-  Descriptions from all associated GenBank mRNAs
  AB049649 - Homo sapiens MRPL32 mRNA for mitochondrial ribosomal protein L32 (L32mt), complete cds.
LF384901 - JP 2014500723-A/192404: Polycomb-Associated Non-Coding RNAs.
MA620478 - JP 2018138019-A/192404: Polycomb-Associated Non-Coding RNAs.
JD215160 - Sequence 196184 from Patent EP1572962.
AK090551 - Homo sapiens cDNA FLJ33232 fis, clone ASTRO2002024, highly similar to 39S ribosomal protein L32, mitochondrial precursor.
AX746505 - Sequence 30 from Patent EP1308459.
BC013147 - Homo sapiens mitochondrial ribosomal protein L32, mRNA (cDNA clone MGC:21626 IMAGE:4340082), complete cds.
AK313393 - Homo sapiens cDNA, FLJ93927, Homo sapiens mitochondrial ribosomal protein L32 (MRPL32), nucleargene encoding mitochondrial protein, mRNA.
CU680524 - Synthetic construct Homo sapiens gateway clone IMAGE:100020169 5' read MRPL32 mRNA.
HQ448324 - Synthetic construct Homo sapiens clone IMAGE:100071742; CCSB000872_03 mitochondrial ribosomal protein L32 (MRPL32) gene, encodes complete protein.
KJ899490 - Synthetic construct Homo sapiens clone ccsbBroadEn_08884 MRPL32 gene, encodes complete protein.
AF161401 - Homo sapiens HSPC283 mRNA, partial cds.
LF330979 - JP 2014500723-A/138482: Polycomb-Associated Non-Coding RNAs.
MA566556 - JP 2018138019-A/138482: Polycomb-Associated Non-Coding RNAs.
LF330977 - JP 2014500723-A/138480: Polycomb-Associated Non-Coding RNAs.
MA566554 - JP 2018138019-A/138480: Polycomb-Associated Non-Coding RNAs.
LF330976 - JP 2014500723-A/138479: Polycomb-Associated Non-Coding RNAs.
MA566553 - JP 2018138019-A/138479: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9BYC8 (Reactome details) participates in the following event(s):

R-HSA-5389839 39S subunit binds 28S subunit:mRNA:fMet-tRNA
R-HSA-5419273 Hydrolysis of GTP and dissociation of 28S and 39S subunits
R-HSA-5389848 TUFM:GTP:aminoacyl-tRNA binds 55S ribosome:mRNA:fMet-tRNA
R-HSA-5389857 Peptide transfer from P-site to A-site (peptide bond formation)
R-HSA-5389842 TUFM hydrolyzes GTP and TUFM:GDP dissociates from 55S ribosome
R-HSA-5419261 55S ribosome with peptidyl-tRNA in A site binds GFM1:GTP
R-HSA-5419281 MRRF binds 55S ribosome:mRNA:tRNA
R-HSA-5419279 Translocation of peptidyl-tRNA from A-site to P-site (and translocation of 55S ribosome by 3 bases along mRNA)
R-HSA-5419264 MTRF1L (MTRF1a) or ICT1 binds stop codon in 55S ribosome:mRNA:peptidyl-tRNA
R-HSA-5419277 GFM2:GTP binds 55S ribosome:mRNA:tRNA:MRRF releasing mRNA and tRNA
R-HSA-5368286 Mitochondrial translation initiation
R-HSA-5419276 Mitochondrial translation termination
R-HSA-5368287 Mitochondrial translation
R-HSA-5389840 Mitochondrial translation elongation
R-HSA-72766 Translation
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000223324.1, ENST00000223324.2, HSPC283, NR_156497, Q96Q68, Q9BYC8, Q9P098, RM32_HUMAN, uc003tia.1, uc003tia.2, uc003tia.3, uc003tia.4, uc003tia.5
UCSC ID: ENST00000223324.3
RefSeq Accession: NM_031903
Protein: Q9BYC8 (aka RM32_HUMAN)
CCDS: CCDS5468.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.