Human Gene TPST1 (ENST00000304842.6) from GENCODE V44
  Description: Homo sapiens tyrosylprotein sulfotransferase 1 (TPST1), mRNA. (from RefSeq NM_003596)
Gencode Transcript: ENST00000304842.6
Gencode Gene: ENSG00000169902.15
Transcript (Including UTRs)
   Position: hg38 chr7:66,205,334-66,360,443 Size: 155,110 Total Exon Count: 6 Strand: +
Coding Region
   Position: hg38 chr7:66,240,426-66,356,842 Size: 116,417 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:66,205,334-66,360,443)mRNA (may differ from genome)Protein (370 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TPST1_HUMAN
DESCRIPTION: RecName: Full=Protein-tyrosine sulfotransferase 1; EC=2.8.2.20; AltName: Full=Tyrosylprotein sulfotransferase 1; Short=TPST-1;
FUNCTION: Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides.
CATALYTIC ACTIVITY: 3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate.
SUBCELLULAR LOCATION: Golgi apparatus membrane; Single-pass type II membrane protein (By similarity).
SIMILARITY: Belongs to the protein sulfotransferase family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: TPST1
Diseases sorted by gene-association score: shwachman-diamond syndrome (4)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 23.77 RPKM in Cervix - Endocervix
Total median expression: 409.94 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -120.60290-0.416 Picture PostScript Text
3' UTR -157.70578-0.273 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR026634 - TPST

ModBase Predicted Comparative 3D Structure on O60507
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0008476 protein-tyrosine sulfotransferase activity
GO:0016740 transferase activity
GO:0042803 protein homodimerization activity

Biological Process:
GO:0006478 peptidyl-tyrosine sulfation
GO:0006954 inflammatory response
GO:0050427 3'-phosphoadenosine 5'-phosphosulfate metabolic process

Cellular Component:
GO:0000139 Golgi membrane
GO:0005794 Golgi apparatus
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0030173 integral component of Golgi membrane


-  Descriptions from all associated GenBank mRNAs
  KJ892538 - Synthetic construct Homo sapiens clone ccsbBroadEn_01932 TPST1 gene, encodes complete protein.
BC013188 - Homo sapiens tyrosylprotein sulfotransferase 1, mRNA (cDNA clone MGC:9140 IMAGE:3918922), complete cds.
AK313098 - Homo sapiens cDNA, FLJ93583, Homo sapiens tyrosylprotein sulfotransferase 1 (TPST1), mRNA.
AK307053 - Homo sapiens cDNA, FLJ97001.
AF038009 - Homo sapiens tyrosylprotein sulfotransferase-1 mRNA, complete cds.
AB590230 - Synthetic construct DNA, clone: pFN21AE1523, Homo sapiens TPST1 gene for tyrosylprotein sulfotransferase 1, without stop codon, in Flexi system.
CR542060 - Homo sapiens full open reading frame cDNA clone RZPDo834D1036D for gene TPST1, tyrosylprotein sulfotransferase 1; complete cds, without stopcodon.
DQ894248 - Synthetic construct Homo sapiens clone IMAGE:100008708; FLH169018.01L; RZPDo839F0893D tyrosylprotein sulfotransferase 1 (TPST1) gene, encodes complete protein.
CR542080 - Homo sapiens full open reading frame cDNA clone RZPDo834F1236D for gene TPST1, tyrosylprotein sulfotransferase 1; complete cds, incl. stopcodon.
DQ893563 - Synthetic construct clone IMAGE:100006193; FLH169022.01X; RZPDo839F0894D tyrosylprotein sulfotransferase 1 (TPST1) gene, encodes complete protein.
JD419566 - Sequence 400590 from Patent EP1572962.
JD439168 - Sequence 420192 from Patent EP1572962.
JD143142 - Sequence 124166 from Patent EP1572962.
JD025731 - Sequence 6755 from Patent EP1572962.
JD029228 - Sequence 10252 from Patent EP1572962.
JD026130 - Sequence 7154 from Patent EP1572962.
JD027827 - Sequence 8851 from Patent EP1572962.
JD346908 - Sequence 327932 from Patent EP1572962.
JD496758 - Sequence 477782 from Patent EP1572962.
AK095722 - Homo sapiens cDNA FLJ38403 fis, clone FEBRA2008282.
JD162331 - Sequence 143355 from Patent EP1572962.
JD294430 - Sequence 275454 from Patent EP1572962.
JD496091 - Sequence 477115 from Patent EP1572962.
JD053030 - Sequence 34054 from Patent EP1572962.
JD093239 - Sequence 74263 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein O60507 (Reactome details) participates in the following event(s):

R-HSA-8954262 TPST1,2 transfer SO4(2-) from PAPS to PODXL2
R-HSA-156584 Cytosolic sulfonation of small molecules
R-HSA-156580 Phase II - Conjugation of compounds
R-HSA-211859 Biological oxidations
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000304842.1, ENST00000304842.2, ENST00000304842.3, ENST00000304842.4, ENST00000304842.5, NM_003596, O60507, Q6FGM7, TPST1_HUMAN, uc003tuw.1, uc003tuw.2, uc003tuw.3, uc003tuw.4, uc003tuw.5
UCSC ID: ENST00000304842.6
RefSeq Accession: NM_003596
Protein: O60507 (aka TPST1_HUMAN)
CCDS: CCDS5533.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.