Human Gene TRIM74 (ENST00000395244.5) from GENCODE V44
  Description: Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q86UV6-1; Sequence=Displayed; Name=2; IsoId=Q86UV6-2; Sequence=VSP_032087; Note=No experimental confirmation available; (from UniProt Q86UV6)
Gencode Transcript: ENST00000395244.5
Gencode Gene: ENSG00000155428.12
Transcript (Including UTRs)
   Position: hg38 chr7:72,959,485-72,966,953 Size: 7,469 Total Exon Count: 5 Strand: -
Coding Region
   Position: hg38 chr7:72,959,882-72,966,157 Size: 6,276 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersRNA-Seq ExpressionMicroarray Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther Names
Methods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:72,959,485-72,966,953)mRNA (may differ from genome)Protein (249 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblExonPrimerGencodeGeneCardsHGNC
HPRDLynxMalacardsMGIneXtProtPubMed
UniProtKB

-  Comments and Description Text from UniProtKB
  ID: TRI74_HUMAN
DESCRIPTION: RecName: Full=Tripartite motif-containing protein 74; AltName: Full=Tripartite motif-containing protein 50C;
SIMILARITY: Belongs to the TRIM/RBCC family.
SIMILARITY: Contains 1 B box-type zinc finger.
SIMILARITY: Contains 1 RING-type zinc finger.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 5.79 RPKM in Testis
Total median expression: 48.96 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -51.50139-0.371 Picture PostScript Text
3' UTR -136.20397-0.343 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000315 - Znf_B-box
IPR001841 - Znf_RING
IPR013083 - Znf_RING/FYVE/PHD
IPR017907 - Znf_RING_CS

Pfam Domains:
PF00643 - B-box zinc finger

ModBase Predicted Comparative 3D Structure on Q86UV6
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0046872 metal ion binding

Cellular Component:
GO:0005622 intracellular
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  AK309306 - Homo sapiens cDNA, FLJ99347.
BC073926 - Homo sapiens tripartite motif-containing 73, mRNA (cDNA clone IMAGE:5193507).
AK126632 - Homo sapiens cDNA FLJ44673 fis, clone BRACE3007058.
BC033871 - Homo sapiens tripartite motif-containing 74, mRNA (cDNA clone MGC:45440 IMAGE:5201777), complete cds.
AF498998 - Homo sapiens tripartite motif protein TRIM50B (TRIM50B) mRNA, complete cds.
AF498999 - Homo sapiens tripartite motif protein TRIM50C (TRIM50C) mRNA, complete cds.
KJ904245 - Synthetic construct Homo sapiens clone ccsbBroadEn_13639 TRIM74 gene, encodes complete protein.
BC156766 - Synthetic construct Homo sapiens clone IMAGE:100062300, MGC:190236 tripartite motif-containing 73 (TRIM73) mRNA, encodes complete protein.
DQ890814 - Synthetic construct clone IMAGE:100003444; FLH166156.01X; RZPDo839D0686D tripartite motif-containing 74 (TRIM74) gene, encodes complete protein.
BC152800 - Synthetic construct Homo sapiens clone IMAGE:100016031, MGC:184197 tripartite motif-containing 73 (TRIM73) mRNA, encodes complete protein.
DQ893970 - Synthetic construct Homo sapiens clone IMAGE:100008430; FLH166152.01L; RZPDo839D0685D tripartite motif-containing 74 (TRIM74) gene, encodes complete protein.
JD476246 - Sequence 457270 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: B7WP46, BC033871, ENST00000395244.1, ENST00000395244.2, ENST00000395244.3, ENST00000395244.4, Q86UV6, TRI74_HUMAN, TRIM50C, uc003tws.1
UCSC ID: ENST00000395244.5
RefSeq Accession: NM_001317815
Protein: Q86UV6 (aka TRI74_HUMAN)
CCDS: CCDS5545.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.