Human Gene SRPK2 (ENST00000393651.8) from GENCODE V44
  Description: Homo sapiens SRSF protein kinase 2 (SRPK2), transcript variant 1, mRNA. (from RefSeq NM_182692)
Gencode Transcript: ENST00000393651.8
Gencode Gene: ENSG00000135250.17
Transcript (Including UTRs)
   Position: hg38 chr7:105,116,374-105,388,921 Size: 272,548 Total Exon Count: 16 Strand: -
Coding Region
   Position: hg38 chr7:105,117,838-105,388,806 Size: 270,969 Coding Exon Count: 16 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:105,116,374-105,388,921)mRNA (may differ from genome)Protein (699 aa)
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-  Comments and Description Text from UniProtKB
  ID: SRPK2_HUMAN
DESCRIPTION: RecName: Full=SRSF protein kinase 2; EC=2.7.11.1; AltName: Full=SFRS protein kinase 2; AltName: Full=Serine/arginine-rich protein-specific kinase 2; Short=SR-protein-specific kinase 2; Contains: RecName: Full=SRSF protein kinase 2 N-terminal; Contains: RecName: Full=SRSF protein kinase 2 C-terminal;
FUNCTION: Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up- regulation. Plays an essential role in splicesomal B complex formation via the phosphorylation of DDX23/PRP28. Can mediate hepatitis B virus (HBV) core protein phosphorylation. Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium.
ENZYME REGULATION: Activated by phosphorylation on Ser-52 and Ser- 588 (By similarity).
SUBUNIT: Interacts with PKB/AKT1 in a phosphorylation-dependent manner. The phosphorylated form (by PKB/AKT1) interacts with YWHAB and YWHAE. Interaction with YWHAB suppresses its cleavage by caspases and inhibits the release of its N-terminal pro-apoptotic fragment. Interacts with SFN. Associates with U4/U6-U5 tri-small nuclear ribonucleoproteins (U4/U6-U5 tri-snRNPs).
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm. KAT5/TIP60 inhibits its nuclear translocation. Phosphorylation at Thr-492 by PKB/AKT1 promotes nuclear translocation.
TISSUE SPECIFICITY: Highly expressed in brain, moderately expressed in heart and skeletal muscle and at low levels in lung, liver, and kidney.
PTM: Phosphorylation at Thr-492 by PKB/AKT1 enhances its stimulatory activity in triggering cyclin-D1 (CCND1) expression and promoting apoptosis in neurons, which can be blocked by YWHAB. It also enhances its protein kinase activity toward ACIN1 and SRSF2, promotes its nuclear translocation and prevents its proteolytic cleavage.
PTM: Proteolytically cleaved at Asp-139 and Asp-403 by caspase-3 during apoptotic cell death. Cleavage at Asp-139 which is the major site of cleavage, produces a small N-terminal fragment that translocates into nucleus and promotes VP16-induced apoptosis.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.
SIMILARITY: Contains 1 protein kinase domain.
SEQUENCE CAUTION: Sequence=AAQ63886.1; Type=Miscellaneous discrepancy; Note=The cDNA appears to contain a duplicated region;

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 55.90 RPKM in Testis
Total median expression: 536.67 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -54.20115-0.471 Picture PostScript Text
3' UTR -336.701464-0.230 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011009 - Kinase-like_dom
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR008271 - Ser/Thr_kinase_AS

Pfam Domains:
PF00069 - Protein kinase domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2X7G - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P78362
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologGenome BrowserNo orthologGenome Browser
Gene Details    Gene Details
Gene Sorter    Gene Sorter
MGIRGD Ensembl SGD
Protein SequenceProtein Sequence Protein Sequence Protein Sequence
AlignmentAlignment Alignment Alignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0000287 magnesium ion binding
GO:0003723 RNA binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0071889 14-3-3 protein binding

Biological Process:
GO:0000245 spliceosomal complex assembly
GO:0001525 angiogenesis
GO:0006397 mRNA processing
GO:0006468 protein phosphorylation
GO:0008284 positive regulation of cell proliferation
GO:0008380 RNA splicing
GO:0010628 positive regulation of gene expression
GO:0016310 phosphorylation
GO:0030154 cell differentiation
GO:0035063 nuclear speck organization
GO:0035556 intracellular signal transduction
GO:0043525 positive regulation of neuron apoptotic process
GO:0045070 positive regulation of viral genome replication
GO:0045071 negative regulation of viral genome replication
GO:0045087 innate immune response
GO:0045787 positive regulation of cell cycle
GO:0048024 regulation of mRNA splicing, via spliceosome

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0005737 cytoplasm
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  AY354201 - Homo sapiens SFRS protein kinase 2 isoform c (SRPK2) mRNA, complete cds, alternatively spliced.
U88666 - Homo sapiens serine kinase SRPK2 mRNA, complete cds.
BC035214 - Homo sapiens SFRS protein kinase 2, mRNA (cDNA clone MGC:24896 IMAGE:4778251), complete cds.
AK093420 - Homo sapiens cDNA FLJ36101 fis, clone TESTI2021118, highly similar to Mus musculus mRNA for SRPK2.
AK092977 - Homo sapiens cDNA FLJ35658 fis, clone SPLEN2013923.
BC068547 - Homo sapiens SFRS protein kinase 2, mRNA (cDNA clone MGC:87435 IMAGE:5265599), complete cds.
DQ891337 - Synthetic construct clone IMAGE:100003967; FLH171199.01X; RZPDo839G09100D SFRS protein kinase 2 (SRPK2) gene, encodes complete protein.
KJ892200 - Synthetic construct Homo sapiens clone ccsbBroadEn_01594 SRPK2 gene, encodes complete protein.
KJ897605 - Synthetic construct Homo sapiens clone ccsbBroadEn_06999 SRPK2 gene, encodes complete protein.
KJ905321 - Synthetic construct Homo sapiens clone ccsbBroadEn_14847 SRPK2 gene, encodes complete protein.
DQ894519 - Synthetic construct Homo sapiens clone IMAGE:100008979; FLH171195.01L; RZPDo839G0999D SFRS protein kinase 2 (SRPK2) gene, encodes complete protein.
AB489157 - Synthetic construct DNA, clone: pF1KB3384, Homo sapiens SRPK2 gene for SFRS protein kinase 2, without stop codon, in Flexi system.
CU690050 - Synthetic construct Homo sapiens gateway clone IMAGE:100018416 5' read SRPK2 mRNA.
CU686663 - Synthetic construct Homo sapiens gateway clone IMAGE:100022833 5' read SRPK2 mRNA.
JD337085 - Sequence 318109 from Patent EP1572962.
JD507218 - Sequence 488242 from Patent EP1572962.
JD353936 - Sequence 334960 from Patent EP1572962.
JD097311 - Sequence 78335 from Patent EP1572962.
JD300035 - Sequence 281059 from Patent EP1572962.
JD434475 - Sequence 415499 from Patent EP1572962.
JD563263 - Sequence 544287 from Patent EP1572962.
JD486550 - Sequence 467574 from Patent EP1572962.
JD164555 - Sequence 145579 from Patent EP1572962.
JD328095 - Sequence 309119 from Patent EP1572962.
JD567092 - Sequence 548116 from Patent EP1572962.
JD562373 - Sequence 543397 from Patent EP1572962.
JD311469 - Sequence 292493 from Patent EP1572962.
JD129991 - Sequence 111015 from Patent EP1572962.
JD053247 - Sequence 34271 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: A8MVX2, ENST00000393651.1, ENST00000393651.2, ENST00000393651.3, ENST00000393651.4, ENST00000393651.5, ENST00000393651.6, ENST00000393651.7, NM_182692, O75220, O75221, P78362, Q6NUL0, Q6V1X2, Q8IYQ3, SRPK2_HUMAN, uc003vcv.1, uc003vcv.2, uc003vcv.3, uc003vcv.4, uc003vcv.5, uc003vcv.6
UCSC ID: ENST00000393651.8
RefSeq Accession: NM_182692
Protein: P78362 (aka SRPK2_HUMAN)
CCDS: CCDS5735.1, CCDS34724.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.