Human Gene RAB33A (ENST00000257017.5) from GENCODE V44
  Description: Homo sapiens RAB33A, member RAS oncogene family (RAB33A), mRNA. (from RefSeq NM_004794)
RefSeq Summary (NM_004794): The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. It is GTP-binding protein and may be involved in vesicle transport. [provided by RefSeq, Jul 2008].
Gencode Transcript: ENST00000257017.5
Gencode Gene: ENSG00000134594.5
Transcript (Including UTRs)
   Position: hg38 chrX:130,171,962-130,184,870 Size: 12,909 Total Exon Count: 2 Strand: +
Coding Region
   Position: hg38 chrX:130,172,063-130,184,740 Size: 12,678 Coding Exon Count: 2 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chrX:130,171,962-130,184,870)mRNA (may differ from genome)Protein (237 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: RB33A_HUMAN
DESCRIPTION: RecName: Full=Ras-related protein Rab-33A; AltName: Full=Small GTP-binding protein S10;
INTERACTION: Q9UI14:RABAC1; NbExp=3; IntAct=EBI-744685, EBI-712367;
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential).
TISSUE SPECIFICITY: Expressed only in lymphoid cell lines.
SIMILARITY: Belongs to the small GTPase superfamily. Rab family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 16.14 RPKM in Brain - Cerebellar Hemisphere
Total median expression: 179.17 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -44.20101-0.438 Picture PostScript Text
3' UTR -15.10130-0.116 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR005225 - Small_GTP-bd_dom
IPR001806 - Small_GTPase
IPR003579 - Small_GTPase_Rab_type

Pfam Domains:
PF00071 - Ras family

ModBase Predicted Comparative 3D Structure on Q14088
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsembl   
Protein SequenceProtein SequenceProtein Sequence   
AlignmentAlignmentAlignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003924 GTPase activity
GO:0005515 protein binding
GO:0005525 GTP binding

Biological Process:
GO:0019882 antigen processing and presentation
GO:2000785 regulation of autophagosome assembly

Cellular Component:
GO:0000139 Golgi membrane
GO:0005886 plasma membrane
GO:0016020 membrane


-  Descriptions from all associated GenBank mRNAs
  D14889 - Homo sapiens mRNA for small GTP-binding protein S10, complete cds.
LF211473 - JP 2014500723-A/18976: Polycomb-Associated Non-Coding RNAs.
MA447050 - JP 2018138019-A/18976: Polycomb-Associated Non-Coding RNAs.
BC001157 - Homo sapiens RAB33A, member RAS oncogene family, mRNA (cDNA clone MGC:1488 IMAGE:3510745), complete cds.
BC001704 - Homo sapiens RAB33A, member RAS oncogene family, mRNA (cDNA clone IMAGE:3502726), containing frame-shift errors.
BC009996 - Homo sapiens RAB33A, member RAS oncogene family, mRNA (cDNA clone MGC:15261 IMAGE:4129324), complete cds.
LF213831 - JP 2014500723-A/21334: Polycomb-Associated Non-Coding RNAs.
MA449408 - JP 2018138019-A/21334: Polycomb-Associated Non-Coding RNAs.
JF432601 - Synthetic construct Homo sapiens clone IMAGE:100073838 RAB33A, member RAS oncogene family (RAB33A) gene, encodes complete protein.
KJ892755 - Synthetic construct Homo sapiens clone ccsbBroadEn_02149 RAB33A gene, encodes complete protein.
BT007038 - Homo sapiens RAB33A, member RAS oncogene family mRNA, complete cds.
AF498959 - Homo sapiens small GTP binding protein RAB33A (RAB33A) mRNA, complete cds.
AK094927 - Homo sapiens cDNA FLJ37608 fis, clone BRCOC2010994, highly similar to RAS-RELATED PROTEIN RAB-33A.
JD056890 - Sequence 37914 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q14088 (Reactome details) participates in the following event(s):

R-HSA-8854612 TBC1D25 accelerates GTP hydrolysis by RAB33B
R-HSA-8870466 RGGT:CHM binds RABs
R-HSA-8870469 RGGT geranylgeranylates RAB proteins
R-HSA-8854214 TBC/RABGAPs
R-HSA-8873719 RAB geranylgeranylation
R-HSA-9007101 Rab regulation of trafficking
R-HSA-597592 Post-translational protein modification
R-HSA-199991 Membrane Trafficking
R-HSA-392499 Metabolism of proteins
R-HSA-5653656 Vesicle-mediated transport

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000257017.1, ENST00000257017.2, ENST00000257017.3, ENST00000257017.4, NM_004794, Q14088, Q5JUZ6, Q92465, RABS10, RB33A_HUMAN, uc004evl.1, uc004evl.2, uc004evl.3, uc004evl.4, uc004evl.5
UCSC ID: ENST00000257017.5
RefSeq Accession: NM_004794
Protein: Q14088 (aka RB33A_HUMAN or R33A_HUMAN)
CCDS: CCDS14621.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.