Mouse Gene Tfap2b (ENSMUST00000027059.10) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus transcription factor AP-2 beta (Tfap2b), transcript variant 1, mRNA. (from RefSeq NM_009334)
Gencode Transcript: ENSMUST00000027059.10
Gencode Gene: ENSMUSG00000025927.13
Transcript (Including UTRs)
   Position: mm10 chr1:19,208,914-19,238,576 Size: 29,663 Total Exon Count: 7 Strand: +
Coding Region
   Position: mm10 chr1:19,209,168-19,234,347 Size: 25,180 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:19,208,914-19,238,576)mRNA (may differ from genome)Protein (459 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
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PubMedReactomeSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: AP2B_MOUSE
DESCRIPTION: RecName: Full=Transcription factor AP-2-beta; Short=AP2-beta; AltName: Full=Activating enhancer-binding protein 2-beta;
FUNCTION: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia.
SUBUNIT: Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with CITED4. Interacts with UBE2I. Interacts with KCTD1; this interaction represses transcription activation. Interacts with CITED2 (via C-terminus); the interaction stimulates TFAP2B-transcriptional activity (By similarity).
SUBCELLULAR LOCATION: Nucleus (Probable).
DEVELOPMENTAL STAGE: Expressed from embryo day 9.5 to birth. In day 13.5 embryo, expressed abundantly in cells coating the neural tube. Expression continues posteriorly in the spinal cord, the dorsal root ganglia, in the prevertebal sympathic ganglia and the ganglion nodosum. High expression found in the dorsal and anteriolateral primordium of the midbrain. Expression also found in skin, kidneys and in many areas of the facial mesenchyme. In adults, expressed in the eye, skin, kidney, prostate, thymus, skeletal muscle and, very weakly in the brain. Highest levels found in kidney.
INDUCTION: During retinoic acid-mediated differentiation.
PTM: Sumoylated on Lys-21; which inhibits transcriptional activity (By similarity).
SIMILARITY: Belongs to the AP-2 family.
SEQUENCE CAUTION: Sequence=CAA55036.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -65.60254-0.258 Picture PostScript Text
3' UTR -1190.704229-0.282 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR004979 - TF_AP2
IPR008122 - TF_AP2_beta
IPR013854 - TF_AP2_C

Pfam Domains:
PF03299 - Transcription factor AP-2

ModBase Predicted Comparative 3D Structure on Q61313
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
GenBankRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000979 RNA polymerase II core promoter sequence-specific DNA binding
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0000983 transcription factor activity, RNA polymerase II core promoter sequence-specific
GO:0001077 transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
GO:0001105 RNA polymerase II transcription coactivator activity
GO:0001106 RNA polymerase II transcription corepressor activity
GO:0001158 enhancer sequence-specific DNA binding
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0003700 transcription factor activity, sequence-specific DNA binding
GO:0003713 transcription coactivator activity
GO:0003714 transcription corepressor activity
GO:0005515 protein binding
GO:0042803 protein homodimerization activity
GO:0043027 cysteine-type endopeptidase inhibitor activity involved in apoptotic process
GO:0043565 sequence-specific DNA binding
GO:0046982 protein heterodimerization activity
GO:0046983 protein dimerization activity

Biological Process:
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0001822 kidney development
GO:0003091 renal water homeostasis
GO:0006006 glucose metabolic process
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006366 transcription from RNA polymerase II promoter
GO:0006915 apoptotic process
GO:0007423 sensory organ development
GO:0008284 positive regulation of cell proliferation
GO:0008285 negative regulation of cell proliferation
GO:0010226 response to lithium ion
GO:0010842 retina layer formation
GO:0010960 magnesium ion homeostasis
GO:0030510 regulation of BMP signaling pathway
GO:0035136 forelimb morphogenesis
GO:0035137 hindlimb morphogenesis
GO:0035810 positive regulation of urine volume
GO:0035909 aorta morphogenesis
GO:0042493 response to drug
GO:0042593 glucose homeostasis
GO:0043066 negative regulation of apoptotic process
GO:0043154 negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0043524 negative regulation of neuron apoptotic process
GO:0043525 positive regulation of neuron apoptotic process
GO:0043588 skin development
GO:0045444 fat cell differentiation
GO:0045595 regulation of cell differentiation
GO:0045892 negative regulation of transcription, DNA-templated
GO:0045893 positive regulation of transcription, DNA-templated
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048485 sympathetic nervous system development
GO:0050796 regulation of insulin secretion
GO:0055062 phosphate ion homeostasis
GO:0055074 calcium ion homeostasis
GO:0055075 potassium ion homeostasis
GO:0055078 sodium ion homeostasis
GO:0072017 distal tubule development
GO:0072044 collecting duct development
GO:0072210 metanephric nephron development
GO:0097070 ductus arteriosus closure
GO:0097275 cellular ammonia homeostasis
GO:0097276 cellular creatinine homeostasis
GO:0097277 cellular urea homeostasis

Cellular Component:
GO:0005634 nucleus


-  Descriptions from all associated GenBank mRNAs
  AK017373 - Mus musculus 6 days neonate head cDNA, RIKEN full-length enriched library, clone:5430431G23 product:transcription factor AP-2 beta, full insert sequence.
AK005128 - Mus musculus adult male cerebellum cDNA, RIKEN full-length enriched library, clone:1500003B06 product:transcription factor AP-2 beta, full insert sequence.
AK028906 - Mus musculus 10 days neonate skin cDNA, RIKEN full-length enriched library, clone:4732469D10 product:transcription factor AP-2 beta, full insert sequence.
X78197 - M.musculus AP-2 beta mRNA.
BC146412 - Synthetic construct Mus musculus clone IMAGE:100015149, MGC:180396 transcription factor AP-2 beta (Tcfap2b) mRNA, encodes complete protein.
BC148852 - Synthetic construct Mus musculus clone IMAGE:100015850, MGC:183462 transcription factor AP-2 beta (Tcfap2b) mRNA, encodes complete protein.
AK032045 - Mus musculus adult male medulla oblongata cDNA, RIKEN full-length enriched library, clone:6330562E04 product:transcription factor AP-2 beta, full insert sequence.
AK035827 - Mus musculus 16 days neonate cerebellum cDNA, RIKEN full-length enriched library, clone:9630008J18 product:transcription factor AP-2 beta, full insert sequence.
AK078046 - Mus musculus adult male medulla oblongata cDNA, RIKEN full-length enriched library, clone:6330400M15 product:TRANSCRIPTION FACTOR AP-2 BETA (AP2-BETA) (ACTIVATING ENHANCER-BINDING PROTEIN 2 BETA) homolog [Mus musculus], full insert sequence.
AK137357 - Mus musculus 16 days neonate cerebellum cDNA, RIKEN full-length enriched library, clone:9630025N04 product:transcription factor AP-2 beta, full insert sequence.
AK053444 - Mus musculus 0 day neonate eyeball cDNA, RIKEN full-length enriched library, clone:E130018K07 product:inferred: transcription factor AP-2 beta, full insert sequence.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q61313 (Reactome details) participates in the following event(s):

R-MMU-8864595 TFAP2B homodimer binds YEATS4
R-MMU-8864278 AP-2 (TFAP2) transcription factors form homo- and heterodimers
R-MMU-8864307 TFAP2 homo- and heterodimers bind CITED and EP300/CREBBP
R-MMU-8864343 KCTD1 binds TFAP2 homo- and heterodimers
R-MMU-8866907 Activation of the TFAP2 (AP-2) family of transcription factors
R-MMU-8864260 Transcriptional regulation by the AP-2 (TFAP2) family of transcription factors
R-MMU-8866904 Negative regulation of activity of TFAP2 (AP-2) family transcription factors
R-MMU-212436 Generic Transcription Pathway
R-MMU-73857 RNA Polymerase II Transcription
R-MMU-74160 Gene expression (Transcription)

-  Other Names for This Gene
  Alternate Gene Symbols: AP2B_MOUSE, NM_009334, Q61313, Q8CEP1, Tcfap2b, uc007akr.1, uc007akr.2, uc007akr.3
UCSC ID: uc007akr.3
RefSeq Accession: NM_009334
Protein: Q61313 (aka AP2B_MOUSE)
CCDS: CCDS14839.1, CCDS78553.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.