Mouse Gene Gpr35 (ENSMUST00000186298.6) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus G protein-coupled receptor 35 (Gpr35), transcript variant 2, mRNA. (from RefSeq NM_001104529)
Gencode Transcript: ENSMUST00000186298.6
Gencode Gene: ENSMUSG00000026271.15
Transcript (Including UTRs)
   Position: mm10 chr1:92,973,122-92,985,044 Size: 11,923 Total Exon Count: 2 Strand: +
Coding Region
   Position: mm10 chr1:92,982,568-92,983,491 Size: 924 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:92,973,122-92,985,044)mRNA (may differ from genome)Protein (307 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: GPR35_MOUSE
DESCRIPTION: RecName: Full=G-protein coupled receptor 35; AltName: Full=Kynurenic acid receptor; Short=KYNA receptor;
FUNCTION: Acts as a receptor for kynurenic acid, an intermediate in the tryptophan metabolic pathway. The activity of this receptor is mediated by G-proteins that elicit calcium mobilization and inositol phosphate production through G(qi/o) proteins.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity).
TISSUE SPECIFICITY: Predominantly expressed in immune and gastrointestinal tissues.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -66.60170-0.392 Picture PostScript Text
3' UTR -511.501553-0.329 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR017452 - GPCR_Rhodpsn_supfam

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)
PF10320 - Serpentine type 7TM GPCR chemoreceptor Srsx

SCOP Domains:
81321 - Family A G protein-coupled receptor-like

ModBase Predicted Comparative 3D Structure on Q9ES90
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
GenBankRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity
GO:0016494 C-X-C chemokine receptor activity

Biological Process:
GO:0007010 cytoskeleton organization
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007204 positive regulation of cytosolic calcium ion concentration
GO:0035025 positive regulation of Rho protein signal transduction
GO:0051482 positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway
GO:0070098 chemokine-mediated signaling pathway
GO:1901386 negative regulation of voltage-gated calcium channel activity
GO:1904456 negative regulation of neuronal action potential

Cellular Component:
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  AK036503 - Mus musculus adult male bone cDNA, RIKEN full-length enriched library, clone:9830121M19 product:PROBABLE G PROTEIN-COUPLED RECEPTOR GPR35, full insert sequence.
AK160972 - Mus musculus 15 days embryo head cDNA, RIKEN full-length enriched library, clone:4022423I09 product:G protein-coupled receptor 35, full insert sequence.
AK154203 - Mus musculus NOD-derived CD11c +ve dendritic cells cDNA, RIKEN full-length enriched library, clone:F630009B18 product:G protein-coupled receptor 35, full insert sequence.
AF200349 - Mus musculus GPR35 mRNA, complete cds.
AK170995 - Mus musculus NOD-derived CD11c +ve dendritic cells cDNA, RIKEN full-length enriched library, clone:F630215J05 product:G protein-coupled receptor 35, full insert sequence.
AK089198 - Mus musculus NOD-derived CD11c +ve dendritic cells cDNA, RIKEN full-length enriched library, clone:F630036B21 product:G protein-coupled receptor 35, full insert sequence.
AK034870 - Mus musculus 12 days embryo embryonic body between diaphragm region and neck cDNA, RIKEN full-length enriched library, clone:9430051L15 product:PROBABLE G PROTEIN-COUPLED RECEPTOR GPR35, full insert sequence.
BC027429 - Mus musculus G protein-coupled receptor 35, mRNA (cDNA clone MGC:35843 IMAGE:3669814), complete cds.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu04080 - Neuroactive ligand-receptor interaction

Reactome (by CSHL, EBI, and GO)

Protein Q9ES90 (Reactome details) participates in the following event(s):

R-MMU-6805205 GPR35 binds kynurenic acid
R-MMU-373076 Class A/1 (Rhodopsin-like receptors)
R-MMU-500792 GPCR ligand binding
R-MMU-372790 Signaling by GPCR
R-MMU-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: GPR35_MOUSE, NM_001104529, Q9ES90, uc007cbz.1, uc007cbz.2, uc007cbz.3
UCSC ID: uc007cbz.3
RefSeq Accession: NM_001104529
Protein: Q9ES90 (aka GPR35_MOUSE or GP35_MOUSE)
CCDS: CCDS15182.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.