Mouse Gene Adamts4 (ENSMUST00000111315.8) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Cleaves aggrecan at the '392-Glu-|-Ala-393' site (By similarity). (from UniProt Q8BNJ2)
RefSeq Summary (NM_172845): This gene encodes a member of 'a disintegrin and metalloproteinase with thrombospondin motifs' (ADAMTS) family of multi-domain matrix-associated metalloendopeptidases that have diverse roles in tissue morphogenesis and pathophysiological remodeling, in inflammation and in vascular biology. The encoded preproprotein undergoes proteolytic processing to generate an active zinc-dependent aggrecanase enzyme that degrades cartilage. [provided by RefSeq, Jul 2016]. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
Gencode Transcript: ENSMUST00000111315.8
Gencode Gene: ENSMUSG00000006403.13
Transcript (Including UTRs)
   Position: mm10 chr1:171,250,421-171,260,637 Size: 10,217 Total Exon Count: 9 Strand: +
Coding Region
   Position: mm10 chr1:171,250,849-171,259,178 Size: 8,330 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:171,250,421-171,260,637)mRNA (may differ from genome)Protein (833 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblExonPrimerGeneCardsMGIPubMed
ReactomeSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: ATS4_MOUSE
DESCRIPTION: RecName: Full=A disintegrin and metalloproteinase with thrombospondin motifs 4; Short=ADAM-TS 4; Short=ADAM-TS4; Short=ADAMTS-4; EC=3.4.24.82; AltName: Full=Aggrecanase-1; Flags: Precursor;
FUNCTION: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Cleaves aggrecan at the '392-Glu-|-Ala-393' site (By similarity).
CATALYTIC ACTIVITY: Glutamyl endopeptidase; bonds cleaved include 370-Thr-Glu-Gly-Glu-|-Ala-Arg-Gly-Ser-377 in the interglobular domain of mammalian aggrecan.
COFACTOR: Binds 1 zinc ion per subunit (By similarity).
SUBUNIT: Interacts with SRPX2 (By similarity).
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).
DOMAIN: The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix (By similarity).
DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM: The precursor is cleaved by a furin endopeptidase (By similarity).
SIMILARITY: Contains 1 disintegrin domain.
SIMILARITY: Contains 1 peptidase M12B domain.
SIMILARITY: Contains 1 TSP type-1 domain.
SEQUENCE CAUTION: Sequence=BAC38944.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -130.37428-0.305 Picture PostScript Text
3' UTR -486.001459-0.333 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR010294 - ADAM_spacer1
IPR024079 - MetalloPept_cat_dom
IPR001590 - Peptidase_M12B
IPR013273 - Peptidase_M12B_ADAM-TS
IPR002870 - Peptidase_M12B_N
IPR000884 - Thrombospondin_1_rpt

Pfam Domains:
PF00090 - Thrombospondin type 1 domain
PF01421 - Reprolysin (M12B) family zinc metalloprotease
PF01562 - Reprolysin family propeptide
PF05986 - ADAM-TS Spacer 1
PF13582 - Metallo-peptidase family M12B Reprolysin-like
PF13688 - Metallo-peptidase family M12

SCOP Domains:
55486 - Metalloproteases ("zincins"), catalytic domain
82895 - TSP-1 type 1 repeat

ModBase Predicted Comparative 3D Structure on Q8BNJ2
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
GenBankRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0002020 protease binding
GO:0004222 metalloendopeptidase activity
GO:0005515 protein binding
GO:0008233 peptidase activity
GO:0008237 metallopeptidase activity
GO:0016787 hydrolase activity
GO:0046872 metal ion binding

Biological Process:
GO:0006508 proteolysis
GO:0042742 defense response to bacterium

Cellular Component:
GO:0005576 extracellular region
GO:0005615 extracellular space
GO:0016607 nuclear speck
GO:0031012 extracellular matrix


-  Descriptions from all associated GenBank mRNAs
  LF193003 - JP 2014500723-A/506: Polycomb-Associated Non-Coding RNAs.
LF193004 - JP 2014500723-A/507: Polycomb-Associated Non-Coding RNAs.
AK028364 - Mus musculus 18 days pregnant adult female placenta and extra embryonic tissue cDNA, RIKEN full-length enriched library, clone:3830423K05 product:a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4, full insert sequence.
AK083534 - Mus musculus 9 days embryo whole body cDNA, RIKEN full-length enriched library, clone:D030041M02 product:a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4, full insert sequence.
AK157287 - Mus musculus activated spleen cDNA, RIKEN full-length enriched library, clone:F830209H24 product:a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4, full insert sequence.
AK164900 - Mus musculus 0 day neonate lung cDNA, RIKEN full-length enriched library, clone:E030010O05 product:a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4, full insert sequence.
LF219629 - JP 2014500723-A/27132: Polycomb-Associated Non-Coding RNAs.
LF219630 - JP 2014500723-A/27133: Polycomb-Associated Non-Coding RNAs.
AK173001 - Mus musculus premature mRNA for mKIAA0688 protein.
LF219631 - JP 2014500723-A/27134: Polycomb-Associated Non-Coding RNAs.
BC027773 - Mus musculus a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 4, mRNA (cDNA clone MGC:38401 IMAGE:5345809), complete cds.
LF219632 - JP 2014500723-A/27135: Polycomb-Associated Non-Coding RNAs.
AK076295 - Mus musculus 0 day neonate skin cDNA, RIKEN full-length enriched library, clone:4632427F01 product:a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4, full insert sequence.
AK156429 - Mus musculus activated spleen cDNA, RIKEN full-length enriched library, clone:F830021G23 product:a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4, full insert sequence.
AF449483 - Mus musculus aggrecanase-1 mRNA, partial cds.
LF219634 - JP 2014500723-A/27137: Polycomb-Associated Non-Coding RNAs.
LF219636 - JP 2014500723-A/27139: Polycomb-Associated Non-Coding RNAs.
AK205486 - Mus musculus cDNA, clone:Y2G0101N13, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000006570, based on BLAT search.
AK216415 - Mus musculus cDNA, clone:Y2G0138E18, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000006570, based on BLAT search.
LF219637 - JP 2014500723-A/27140: Polycomb-Associated Non-Coding RNAs.
LF219638 - JP 2014500723-A/27141: Polycomb-Associated Non-Coding RNAs.
AK195842 - Mus musculus cDNA, clone:Y1G0119M23, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000006570, based on BLAT search.
LF219639 - JP 2014500723-A/27142: Polycomb-Associated Non-Coding RNAs.
LF219640 - JP 2014500723-A/27143: Polycomb-Associated Non-Coding RNAs.
LF219641 - JP 2014500723-A/27144: Polycomb-Associated Non-Coding RNAs.
LF219642 - JP 2014500723-A/27145: Polycomb-Associated Non-Coding RNAs.
MA428580 - JP 2018138019-A/506: Polycomb-Associated Non-Coding RNAs.
MA428581 - JP 2018138019-A/507: Polycomb-Associated Non-Coding RNAs.
MA455206 - JP 2018138019-A/27132: Polycomb-Associated Non-Coding RNAs.
MA455207 - JP 2018138019-A/27133: Polycomb-Associated Non-Coding RNAs.
MA455208 - JP 2018138019-A/27134: Polycomb-Associated Non-Coding RNAs.
MA455209 - JP 2018138019-A/27135: Polycomb-Associated Non-Coding RNAs.
MA455211 - JP 2018138019-A/27137: Polycomb-Associated Non-Coding RNAs.
MA455213 - JP 2018138019-A/27139: Polycomb-Associated Non-Coding RNAs.
MA455214 - JP 2018138019-A/27140: Polycomb-Associated Non-Coding RNAs.
MA455215 - JP 2018138019-A/27141: Polycomb-Associated Non-Coding RNAs.
MA455216 - JP 2018138019-A/27142: Polycomb-Associated Non-Coding RNAs.
MA455217 - JP 2018138019-A/27143: Polycomb-Associated Non-Coding RNAs.
MA455218 - JP 2018138019-A/27144: Polycomb-Associated Non-Coding RNAs.
MA455219 - JP 2018138019-A/27145: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q8BNJ2 (Reactome details) participates in the following event(s):

R-MMU-5173005 B3GALTL transfers glucose to O-fucosyl-proteins
R-MMU-5173192 POFUT2 transfers fucose to TSR domain-containing proteins
R-MMU-3788075 Brevican degradation by ADAMTS4, ADAMTS5
R-MMU-5173214 O-glycosylation of TSR domain-containing proteins
R-MMU-1474228 Degradation of the extracellular matrix
R-MMU-5173105 O-linked glycosylation
R-MMU-1474244 Extracellular matrix organization
R-MMU-597592 Post-translational protein modification
R-MMU-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: AK028364, ATS4_MOUSE, Q8BNJ2, Q8K384, uc007dnn.1, uc007dnn.2
UCSC ID: uc007dnn.2
RefSeq Accession: NM_172845
Protein: Q8BNJ2 (aka ATS4_MOUSE)
CCDS: CCDS15485.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.