Mouse Gene Capn2 (ENSMUST00000068505.9) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus calpain 2 (Capn2), mRNA. (from RefSeq NM_009794)
Gencode Transcript: ENSMUST00000068505.9
Gencode Gene: ENSMUSG00000026509.15
Transcript (Including UTRs)
   Position: mm10 chr1:182,467,260-182,517,524 Size: 50,265 Total Exon Count: 21 Strand: -
Coding Region
   Position: mm10 chr1:182,468,212-182,517,354 Size: 49,143 Coding Exon Count: 21 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:182,467,260-182,517,524)mRNA (may differ from genome)Protein (700 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: CAN2_MOUSE
DESCRIPTION: RecName: Full=Calpain-2 catalytic subunit; EC=3.4.22.53; AltName: Full=80 kDa M-calpain subunit; Short=CALP80; AltName: Full=Calcium-activated neutral proteinase 2; Short=CANP 2; AltName: Full=Calpain M-type; AltName: Full=Calpain-2 large subunit; AltName: Full=Millimolar-calpain; Short=M-calpain; Flags: Precursor;
FUNCTION: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction (By similarity).
CATALYTIC ACTIVITY: Broad endopeptidase specificity.
COFACTOR: Binds 7 calcium ions (By similarity).
ENZYME REGULATION: Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.
SUBUNIT: Forms a heterodimer with a small (regulatory) subunit (CAPNS1).
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell membrane (By similarity). Note=Translocates to the plasma membrane upon Ca(2+) binding (By similarity).
TISSUE SPECIFICITY: Ubiquitous.
SIMILARITY: Belongs to the peptidase C2 family.
SIMILARITY: Contains 1 calpain catalytic domain.
SIMILARITY: Contains 2 EF-hand domains.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -65.50170-0.385 Picture PostScript Text
3' UTR -241.44952-0.254 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR022684 - Calpain_cysteine_protease
IPR022682 - Calpain_domain_III
IPR022683 - Calpain_III
IPR011992 - EF-hand-like_dom
IPR018247 - EF_Hand_1_Ca_BS
IPR018249 - EF_HAND_2
IPR002048 - EF_hand_Ca-bd
IPR000169 - Pept_cys_AS
IPR001300 - Peptidase_C2_calpain_cat

Pfam Domains:
PF00648 - Calpain family cysteine protease
PF01067 - Calpain large subunit, domain III
PF13833 - EF-hand domain pair

SCOP Domains:
47473 - EF-hand
49758 - Calpain large subunit, middle domain (domain III)
54001 - Cysteine proteinases

ModBase Predicted Comparative 3D Structure on O08529
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
GenBankRGDEnsembl   
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004198 calcium-dependent cysteine-type endopeptidase activity
GO:0005509 calcium ion binding
GO:0008092 cytoskeletal protein binding
GO:0008233 peptidase activity
GO:0008234 cysteine-type peptidase activity
GO:0016787 hydrolase activity
GO:0046872 metal ion binding
GO:0046982 protein heterodimerization activity

Biological Process:
GO:0001666 response to hypoxia
GO:0001824 blastocyst development
GO:0006508 proteolysis
GO:0007520 myoblast fusion
GO:0016540 protein autoprocessing
GO:0051493 regulation of cytoskeleton organization
GO:0051603 proteolysis involved in cellular protein catabolic process
GO:0071230 cellular response to amino acid stimulus

Cellular Component:
GO:0000785 chromatin
GO:0005622 intracellular
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005764 lysosome
GO:0005783 endoplasmic reticulum
GO:0005794 Golgi apparatus
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0030425 dendrite
GO:0031143 pseudopodium
GO:0045121 membrane raft
GO:0097038 perinuclear endoplasmic reticulum


-  Descriptions from all associated GenBank mRNAs
  AK154855 - Mus musculus NOD-derived CD11c +ve dendritic cells cDNA, RIKEN full-length enriched library, clone:F630109M08 product:calpain 2, full insert sequence.
BC054726 - Mus musculus calpain 2, mRNA (cDNA clone MGC:65596 IMAGE:5703563), complete cds.
D38117 - Mus musculus mRNA for m-calpain large subunit, complete cds.
AK150695 - Mus musculus bone marrow macrophage cDNA, RIKEN full-length enriched library, clone:I830013M09 product:calpain 2, full insert sequence.
AK152094 - Mus musculus bone marrow macrophage cDNA, RIKEN full-length enriched library, clone:I830047J04 product:calpain 2, full insert sequence.
AK152790 - Mus musculus bone marrow macrophage cDNA, RIKEN full-length enriched library, clone:I830086C02 product:calpain 2, full insert sequence.
AK152994 - Mus musculus bone marrow macrophage cDNA, RIKEN full-length enriched library, clone:I830099I24 product:calpain 2, full insert sequence.
AK052141 - Mus musculus 12 days embryo eyeball cDNA, RIKEN full-length enriched library, clone:D230050I17 product:calpain 2, full insert sequence.
AK200761 - Mus musculus cDNA, clone:Y1G0135N09, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000027789, based on BLAT search.
AK192389 - Mus musculus cDNA, clone:Y1G0108L23, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000027789, based on BLAT search.
AK203804 - Mus musculus cDNA, clone:Y1G0146E04, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000027789, based on BLAT search.
AK191235 - Mus musculus cDNA, clone:Y1G0105E06, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000027789, based on BLAT search.
AF015038 - Mus musculus 80kDa m-calpain subunit (calp80) mRNA, complete cds.
AK198770 - Mus musculus cDNA, clone:Y1G0129C07, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000027789, based on BLAT search.
AK203361 - Mus musculus cDNA, clone:Y1G0144K06, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000027789, based on BLAT search.
AK208163 - Mus musculus cDNA, clone:Y2G0110F03, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000027789, based on BLAT search.
AK219579 - Mus musculus cDNA, clone:Y2G0148O11, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000027789, based on BLAT search.
AK178489 - Mus musculus cDNA, clone:Y0G0106P03, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000027789, based on BLAT search.
AK189547 - Mus musculus cDNA, clone:Y0G0149J03, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000027789, based on BLAT search.
AK216791 - Mus musculus cDNA, clone:Y2G0139I06, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000027789, based on BLAT search.
AK212305 - Mus musculus cDNA, clone:Y2G0124I06, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000027789, based on BLAT search.
Y10139 - M.musculus mRNA for m-calpain.
AK183796 - Mus musculus cDNA, clone:Y0G0127E01, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000068505, based on BLAT search.
AK207216 - Mus musculus cDNA, clone:Y2G0107E23, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000068505, based on BLAT search.
AK183766 - Mus musculus cDNA, clone:Y0G0127B15, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000027789, based on BLAT search.
AK183150 - Mus musculus cDNA, clone:Y0G0124N23, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000027789, based on BLAT search.
AK195491 - Mus musculus cDNA, clone:Y1G0118K15, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000027789, based on BLAT search.
AK199295 - Mus musculus cDNA, clone:Y1G0130M01, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000027789, based on BLAT search.
AK182739 - Mus musculus cDNA, clone:Y0G0123J08, strand:unspecified.
AK198879 - Mus musculus cDNA, clone:Y1G0129H06, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000064217, based on BLAT search.
AK181353 - Mus musculus cDNA, clone:Y0G0117M19, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000027789, based on BLAT search.
AK207878 - Mus musculus cDNA, clone:Y2G0109G15, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000027789, based on BLAT search.
AK078787 - Mus musculus 15 days embryo male testis cDNA, RIKEN full-length enriched library, clone:8030487N24 product:calpain 2, full insert sequence.
AK053832 - Mus musculus 0 day neonate eyeball cDNA, RIKEN full-length enriched library, clone:E130314B09 product:calpain 2, full insert sequence.
AK180270 - Mus musculus cDNA, clone:Y0G0113I19, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000068505, based on BLAT search.
AK184788 - Mus musculus cDNA, clone:Y0G0131I08, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000068505, based on BLAT search.
AK205009 - Mus musculus cDNA, clone:Y1G0150F16, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000068505, based on BLAT search.
AK180789 - Mus musculus cDNA, clone:Y0G0115N16, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000068505, based on BLAT search.
AK196175 - Mus musculus cDNA, clone:Y1G0121A13, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000068505, based on BLAT search.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu04141 - Protein processing in endoplasmic reticulum
mmu04210 - Apoptosis
mmu04510 - Focal adhesion
mmu05010 - Alzheimer's disease

BioCarta from NCI Cancer Genome Anatomy Project
m_mCalpainPathway - mCalpain and friends in Cell motility
m_mef2dPathway - Role of MEF2D in T-cell Apoptosis

Reactome (by CSHL, EBI, and GO)

Protein O08529 (Reactome details) participates in the following event(s):

R-MMU-8863416 Calpain 1 or Calpain 2 cleaves Cdk5r1 (p35)
R-MMU-8848658 CAPN:4xCa2+:CAPNS cleave cytoskeletal proteins
R-MMU-1474228 Degradation of the extracellular matrix
R-MMU-1474244 Extracellular matrix organization

-  Other Names for This Gene
  Alternate Gene Symbols: CAN2_MOUSE, NM_009794, O08529, O35518, O54843, uc007dye.1, uc007dye.2, uc007dye.3
UCSC ID: uc007dye.3
RefSeq Accession: NM_009794
Protein: O08529 (aka CAN2_MOUSE)
CCDS: CCDS35813.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.