Mouse Gene Cdc34 (ENSMUST00000020550.12) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus cell division cycle 34 (Cdc34), transcript variant 1, mRNA. (from RefSeq NM_177613)
Gencode Transcript: ENSMUST00000020550.12
Gencode Gene: ENSMUSG00000020307.15
Transcript (Including UTRs)
   Position: mm10 chr10:79,682,195-79,688,398 Size: 6,204 Total Exon Count: 5 Strand: +
Coding Region
   Position: mm10 chr10:79,682,414-79,688,059 Size: 5,646 Coding Exon Count: 5 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr10:79,682,195-79,688,398)mRNA (may differ from genome)Protein (235 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: UB2R1_MOUSE
DESCRIPTION: RecName: Full=Ubiquitin-conjugating enzyme E2 R1; EC=6.3.2.19; AltName: Full=Ubiquitin-conjugating enzyme E2-32 kDa complementing; AltName: Full=Ubiquitin-conjugating enzyme E2-CDC34; AltName: Full=Ubiquitin-protein ligase R1;
FUNCTION: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5- mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin (By similarity).
CATALYTIC ACTIVITY: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
ENZYME REGULATION: CDC34-catalyzed polyubiquitin chain assembly activity is stimulated by the conjugation of NEDD8 to the CUL1 SCF E3 ligase complex subunit (By similarity).
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT: Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. When cullin is neddylated, the interaction between the E2 and the SCF complex is strengthened (By similarity). When phosphorylated, interacts with beta-TrCP (BTRC) (By similarity). Interacts with casein kinase subunit CSNK2B (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note=The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase (By similarity).
DOMAIN: The C-terminal acidic tail is required for nuclear localization and is involved in the binding to SCF E3 ligase complexes, and more specifically with the CUL1 subunit (By similarity).
PTM: Phosphorylated by CK2. Phosphorylation of the C-terminal tail by CK2 controles the nuclear localization (By similarity).
SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -139.50219-0.637 Picture PostScript Text
3' UTR -109.50339-0.323 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000608 - UBQ-conjugat_E2
IPR023313 - UBQ-conjugating_AS
IPR016135 - UBQ-conjugating_enzyme/RWD

Pfam Domains:
PF00179 - Ubiquitin-conjugating enzyme

SCOP Domains:
54495 - UBC-like

ModBase Predicted Comparative 3D Structure on Q8CFI2
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
GenBankRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004842 ubiquitin-protein transferase activity
GO:0005524 ATP binding
GO:0016740 transferase activity
GO:0031625 ubiquitin protein ligase binding
GO:0061630 ubiquitin protein ligase activity
GO:0061631 ubiquitin conjugating enzyme activity

Biological Process:
GO:0000209 protein polyubiquitination
GO:0006513 protein monoubiquitination
GO:0007049 cell cycle
GO:0016567 protein ubiquitination
GO:0035458 cellular response to interferon-beta
GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0043525 positive regulation of neuron apoptotic process
GO:0043951 negative regulation of cAMP-mediated signaling
GO:0070936 protein K48-linked ubiquitination
GO:0090261 positive regulation of inclusion body assembly

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0016607 nuclear speck


-  Descriptions from all associated GenBank mRNAs
  BC039160 - Mus musculus cell division cycle 34 homolog (S. cerevisiae), mRNA (cDNA clone MGC:25601 IMAGE:4024472), complete cds.
BC094502 - Mus musculus cell division cycle 34 homolog (S. cerevisiae), mRNA (cDNA clone MGC:106235 IMAGE:5034261), complete cds.
AK187836 - Mus musculus cDNA, clone:Y0G0143D09, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000067118, based on BLAT search.
AB341641 - Mus musculus non-coding RNA, oocyte_clustered_small_RNA6842, complete sequence.
AB341642 - Mus musculus non-coding RNA, oocyte_clustered_small_RNA6843, complete sequence.
AK153982 - Mus musculus 2 days neonate thymus thymic cells cDNA, RIKEN full-length enriched library, clone:E430018J24 product:cell division cycle 34 homolog (S. cerevisiae), full insert sequence.
LF229874 - JP 2014500723-A/37377: Polycomb-Associated Non-Coding RNAs.
MA465451 - JP 2018138019-A/37377: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu04120 - Ubiquitin mediated proteolysis

BioCarta from NCI Cancer Genome Anatomy Project
m_fbw7Pathway - Cyclin E Destruction Pathway
m_g2Pathway - Cell Cycle: G2/M Checkpoint
m_skp2e2fPathway - E2F1 Destruction Pathway

Reactome (by CSHL, EBI, and GO)

Protein Q8CFI2 (Reactome details) participates in the following event(s):

R-MMU-8852129 UBA1 conjugates ubiquitin to cytosolic E2 enzymes
R-MMU-983152 Transfer of ubiquitin from E1 to E2
R-MMU-983140 Transfer of Ub from E2 to substrate and release of E2
R-MMU-8852130 UBA1 conjugates ubiquitin to nuclear E2 enzymes
R-MMU-983157 Interaction of E3 with substrate and E2-Ub complex
R-MMU-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
R-MMU-202424 Downstream TCR signaling
R-MMU-2871837 FCERI mediated NF-kB activation
R-MMU-5607764 CLEC7A (Dectin-1) signaling
R-MMU-8852135 Protein ubiquitination
R-MMU-983169 Class I MHC mediated antigen processing & presentation
R-MMU-202403 TCR signaling
R-MMU-2454202 Fc epsilon receptor (FCERI) signaling
R-MMU-5621481 C-type lectin receptors (CLRs)
R-MMU-597592 Post-translational protein modification
R-MMU-1280218 Adaptive Immune System
R-MMU-168249 Innate Immune System
R-MMU-392499 Metabolism of proteins
R-MMU-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: NM_177613, Q505K8, Q8CFI2, UB2R1_MOUSE, Ubch3, Ube2r1, uc007fzi.1
UCSC ID: uc007fzi.1
RefSeq Accession: NM_177613
Protein: Q8CFI2 (aka UB2R1_MOUSE or UBC3_MOUSE)
CCDS: CCDS23983.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.