ID:UB2R1_MOUSE DESCRIPTION: RecName: Full=Ubiquitin-conjugating enzyme E2 R1; EC=6.3.2.19; AltName: Full=Ubiquitin-conjugating enzyme E2-32 kDa complementing; AltName: Full=Ubiquitin-conjugating enzyme E2-CDC34; AltName: Full=Ubiquitin-protein ligase R1; FUNCTION: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5- mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin (By similarity). CATALYTIC ACTIVITY: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine. ENZYME REGULATION: CDC34-catalyzed polyubiquitin chain assembly activity is stimulated by the conjugation of NEDD8 to the CUL1 SCF E3 ligase complex subunit (By similarity). PATHWAY: Protein modification; protein ubiquitination. SUBUNIT: Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. When cullin is neddylated, the interaction between the E2 and the SCF complex is strengthened (By similarity). When phosphorylated, interacts with beta-TrCP (BTRC) (By similarity). Interacts with casein kinase subunit CSNK2B (By similarity). SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note=The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase (By similarity). DOMAIN: The C-terminal acidic tail is required for nuclear localization and is involved in the binding to SCF E3 ligase complexes, and more specifically with the CUL1 subunit (By similarity). PTM: Phosphorylated by CK2. Phosphorylation of the C-terminal tail by CK2 controles the nuclear localization (By similarity). SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q8CFI2
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0000166 nucleotide binding GO:0004842 ubiquitin-protein transferase activity GO:0005524 ATP binding GO:0016740 transferase activity GO:0031625 ubiquitin protein ligase binding GO:0061630 ubiquitin protein ligase activity GO:0061631 ubiquitin conjugating enzyme activity
Biological Process: GO:0000209 protein polyubiquitination GO:0006513 protein monoubiquitination GO:0007049 cell cycle GO:0016567 protein ubiquitination GO:0035458 cellular response to interferon-beta GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043525 positive regulation of neuron apoptotic process GO:0043951 negative regulation of cAMP-mediated signaling GO:0070936 protein K48-linked ubiquitination GO:0090261 positive regulation of inclusion body assembly