Mouse Gene Aldh1l2 (ENSMUST00000020497.13) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus aldehyde dehydrogenase 1 family, member L2 (Aldh1l2), mRNA. (from RefSeq NM_153543)
Gencode Transcript: ENSMUST00000020497.13
Gencode Gene: ENSMUSG00000020256.14
Transcript (Including UTRs)
   Position: mm10 chr10:83,487,450-83,534,140 Size: 46,691 Total Exon Count: 23 Strand: -
Coding Region
   Position: mm10 chr10:83,490,579-83,534,014 Size: 43,436 Coding Exon Count: 23 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr10:83,487,450-83,534,140)mRNA (may differ from genome)Protein (923 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: AL1L2_MOUSE
DESCRIPTION: RecName: Full=Mitochondrial 10-formyltetrahydrofolate dehydrogenase; Short=Mitochondrial 10-FTHFDH; Short=mtFDH; EC=1.5.1.6; AltName: Full=Aldehyde dehydrogenase family 1 member L2;
CATALYTIC ACTIVITY: 10-formyltetrahydrofolate + NADP(+) + H(2)O = tetrahydrofolate + CO(2) + NADPH.
SUBCELLULAR LOCATION: Mitochondrion (By similarity).
SIMILARITY: In the N-terminal section; belongs to the GART family.
SIMILARITY: In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.
SIMILARITY: Contains 1 acyl carrier domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -45.30126-0.360 Picture PostScript Text
3' UTR -886.003129-0.283 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011407 - 10_FTHF_DH
IPR009081 - Acyl_carrier_prot-like
IPR016161 - Ald_DH/histidinol_DH
IPR016163 - Ald_DH_C
IPR016160 - Ald_DH_CS
IPR016162 - Ald_DH_N
IPR015590 - Aldehyde_DH_dom
IPR005793 - Formyl_trans_C
IPR002376 - Formyl_transf_N
IPR011034 - Formyl_transferase_C-like
IPR001555 - GART_AS
IPR006162 - PPantetheine_attach_site

Pfam Domains:
PF00171 - Aldehyde dehydrogenase family
PF00550 - Phosphopantetheine attachment site
PF00551 - Formyl transferase
PF02911 - Formyl transferase, C-terminal domain

SCOP Domains:
47336 - ACP-like
88633 - Positive stranded ssRNA viruses
50486 - FMT C-terminal domain-like
53328 - Formyltransferase
53720 - ALDH-like

ModBase Predicted Comparative 3D Structure on Q8K009
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
GenBankRGDEnsembl   
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003824 catalytic activity
GO:0004029 aldehyde dehydrogenase (NAD) activity
GO:0016155 formyltetrahydrofolate dehydrogenase activity
GO:0016491 oxidoreductase activity
GO:0016620 oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
GO:0016742 hydroxymethyl-, formyl- and related transferase activity

Biological Process:
GO:0006730 one-carbon metabolic process
GO:0008152 metabolic process
GO:0009058 biosynthetic process
GO:0009258 10-formyltetrahydrofolate catabolic process
GO:0055114 oxidation-reduction process

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005739 mitochondrion


-  Descriptions from all associated GenBank mRNAs
  BC034531 - Mus musculus aldehyde dehydrogenase 1 family, member L2, mRNA (cDNA clone MGC:19039 IMAGE:4188055), complete cds.
AK141954 - Mus musculus 12 days embryo spinal ganglion cDNA, RIKEN full-length enriched library, clone:D130074P12 product:Similar to 10-formyltetrahydrofolate dehydrogenase, full insert sequence.
AK145046 - Mus musculus mammary gland RCB-0526 Jyg-MC(A) cDNA, RIKEN full-length enriched library, clone:G830031C04 product:similar to 10-formyltetrahydrofolate dehydrogenase (Fragment) [Mus musculus], full insert sequence.
AK052361 - Mus musculus 13 days embryo heart cDNA, RIKEN full-length enriched library, clone:D330038I09 product:similar to 10-FORMYLTETRAHYDROFOLATE DEHYDROGENASE (EC 1.5.1.6) (10-FTHFDH) (FBP-CI) [Rattus norvegicus], full insert sequence.
AK203808 - Mus musculus cDNA, clone:Y1G0146E09, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000020497, based on BLAT search.
AK144594 - Mus musculus lung RCB-0558 LLC cDNA, RIKEN full-length enriched library, clone:G730006P05 product:hypothetical protein, full insert sequence.
AK086413 - Mus musculus 15 days embryo head cDNA, RIKEN full-length enriched library, clone:D930026K06 product:weakly similar to 10-FORMYLTETRAHYDROFOLATE DEHYDROGENASE (EC 1.5.1.6) (10-FTHFDH) [Homo sapiens], full insert sequence.
AK188745 - Mus musculus cDNA, clone:Y0G0146F13, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000020497, based on BLAT search.
AK208193 - Mus musculus cDNA, clone:Y2G0110G13, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000020497, based on BLAT search.
AK215056 - Mus musculus cDNA, clone:Y2G0133N07, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000020497, based on BLAT search.
AK203320 - Mus musculus cDNA, clone:Y1G0144H24, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000020497, based on BLAT search.
AK192503 - Mus musculus cDNA, clone:Y1G0109B14, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000020497, based on BLAT search.
AK201197 - Mus musculus cDNA, clone:Y1G0137G04, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000020497, based on BLAT search.
AK046198 - Mus musculus adult male corpora quadrigemina cDNA, RIKEN full-length enriched library, clone:B230350I06 product:hypothetical protein, full insert sequence.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu00670 - One carbon pool by folate

Reactome (by CSHL, EBI, and GO)

Protein Q8K009 (Reactome details) participates in the following event(s):

R-MMU-6808487 ALDH1L2 dehydrogenates 10-formyl-THFPG to THFPG
R-MMU-196757 Metabolism of folate and pterines
R-MMU-196849 Metabolism of water-soluble vitamins and cofactors
R-MMU-196854 Metabolism of vitamins and cofactors
R-MMU-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: AL1L2_MOUSE, E9QLV8, NM_153543, Q8K009, uc007gkh.1, uc007gkh.2, uc007gkh.3
UCSC ID: uc007gkh.3
RefSeq Accession: NM_153543
Protein: Q8K009 (aka AL1L2_MOUSE)
CCDS: CCDS24077.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.