Mouse Gene Rack1 (ENSMUST00000020640.7) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus receptor for activated C kinase 1 (Rack1), mRNA. (from RefSeq NM_008143)
Gencode Transcript: ENSMUST00000020640.7
Gencode Gene: ENSMUSG00000020372.15
Transcript (Including UTRs)
   Position: mm10 chr11:48,800,360-48,806,434 Size: 6,075 Total Exon Count: 8 Strand: +
Coding Region
   Position: mm10 chr11:48,800,464-48,806,045 Size: 5,582 Coding Exon Count: 8 

Page IndexSequence and LinksUniProtKB CommentsPrimersRNA StructureProtein Structure
Other SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther NamesModel Information
Methods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:48,800,360-48,806,434)mRNA (may differ from genome)Protein (317 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: GBLP_MOUSE
DESCRIPTION: RecName: Full=Guanine nucleotide-binding protein subunit beta-2-like 1; AltName: Full=12-3; AltName: Full=Receptor for activated C kinase; AltName: Full=Receptor of activated protein kinase C 1; Short=RACK1; AltName: Full=p205;
FUNCTION: Involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression. Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand- independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration.
SUBUNIT: Component of the small (40S) ribosomal subunit. Exists as a monomer and also forms oligomers. Binds SLC9A3R1. Forms a ternary complex with TRIM63 and PRKCE. Interacts with HABP4, KRT1 and OTUB1. Interacts with SRC (via SH2 domain); the interaction is enhanced by tyrosine phosphorylation of GNB2L1/RACK1. Recruited in a circadian manner into a nuclear complex which also includes BMAL1 and PRKCA. Interacts with AR. Interacts with IGF1R but not with INSR. Interacts with ADAM12. Interacts with CLEC1B (via N- terminal region) and with HIF1A; the interaction promotes their degradation. Interacts with RHOA; this enhances RHOA activation and promotes cell migration. Interacts with CHRM2; the interaction regulates CHRM2 internalization. Interacts with TRPM6 (via kinase domain). Interacts with PTK2/FAK1; required for PTK2/FAK1 phosphorylation and dephosphorylation. Interacts with FLT1. Interacts with HRAS.
INTERACTION: O88351:Ikbkb; NbExp=4; IntAct=EBI-296749, EBI-447960;
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein (By similarity). Cytoplasm (By similarity). Cytoplasm, perinuclear region (By similarity). Cytoplasm, cytoskeleton (By similarity). Nucleus. Perikaryon. Cell projection, dendrite. Note=Recruited to the plasma membrane through interaction with KRT1 which binds to membrane-bound ITGB1. PKC activation induces translocation from the perinuclear region to the cell periphery. In the brain, detected mainly in cell bodies and dendrites with little expression in axonal fibers or nuclei (By similarity).
TISSUE SPECIFICITY: Strongly and ubiquitously expressed in the embryonic and early postnatal brain. At E11.5, expressed in a high-dorsal to low-ventral gradient throughout the brain. At E13.5, most abundant in the telecephalon. At E18.5, expressed most abundantly in layers 1-4 of the cortex, striatum, hippocampus, dentate gyrus, and specific thalamic nuclei. This expression decreases during postnatal development and is localized in the dentate gyrus, habenula, piriform cortex, paraventricular nucleus of the hypothalamus and supraoptic nucleus of the adult brain.
DEVELOPMENTAL STAGE: Expressed throughout embryonic brain development with high levels detected at E11.5, E13.5 and E18.5. Also detected at high levels in the adult brain.
PTM: Phosphorylated on Tyr-228 and/or Tyr-246 by SRC. This is required for binding to SRC (By similarity).
SIMILARITY: Belongs to the WD repeat G protein beta family.
SIMILARITY: Contains 7 WD repeats.
SEQUENCE CAUTION: Sequence=AAG29506.1; Type=Frameshift; Positions=232, 239;

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

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Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -42.20104-0.406 Picture PostScript Text
3' UTR -96.00389-0.247 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR020472 - G-protein_beta_WD-40_rep
IPR015943 - WD40/YVTN_repeat-like_dom
IPR001680 - WD40_repeat
IPR019775 - WD40_repeat_CS
IPR017986 - WD40_repeat_dom

Pfam Domains:
PF00400 - WD domain, G-beta repeat

SCOP Domains:
50952 - Soluble quinoprotein glucose dehydrogenase
50956 - Thermostable phytase (3-phytase)
50960 - TolB, C-terminal domain
63825 - YWTD domain
63829 - Calcium-dependent phosphotriesterase
69304 - Tricorn protease N-terminal domain
101898 - NHL repeat
50965 - Galactose oxidase, central domain
75011 - 3-carboxy-cis,cis-mucoante lactonizing enzyme
101908 - Putative isomerase YbhE
117289 - Nucleoporin domain
50969 - YVTN repeat-like/Quinoprotein amine dehydrogenase
50974 - Nitrous oxide reductase, N-terminal domain
50978 - WD40 repeat-like
50993 - Peptidase/esterase 'gauge' domain
69322 - Tricorn protease domain 2
50998 - Quinoprotein alcohol dehydrogenase-like
51004 - C-terminal (heme d1) domain of cytochrome cd1-nitrite reductase
82171 - DPP6 N-terminal domain-like
55048 - Probable ACP-binding domain of malonyl-CoA ACP transacylase

ModBase Predicted Comparative 3D Structure on P68040
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologGenome BrowserGenome BrowserNo ortholog
Gene Details    Gene Details
Gene Sorter    Gene Sorter
GenBankRGDEnsemblEnsemblWormBaseSGD
Protein SequenceProtein Sequence Protein Sequence  
AlignmentAlignment Alignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005080 protein kinase C binding
GO:0005515 protein binding
GO:0008200 ion channel inhibitor activity
GO:0008656 cysteine-type endopeptidase activator activity involved in apoptotic process
GO:0019899 enzyme binding
GO:0019903 protein phosphatase binding
GO:0030292 protein tyrosine kinase inhibitor activity
GO:0030332 cyclin binding
GO:0030971 receptor tyrosine kinase binding
GO:0035591 signaling adaptor activity
GO:0038023 signaling receptor activity
GO:0042169 SH2 domain binding
GO:0042803 protein homodimerization activity
GO:0043022 ribosome binding
GO:0045296 cadherin binding

Biological Process:
GO:0001934 positive regulation of protein phosphorylation
GO:0006412 translation
GO:0006417 regulation of translation
GO:0006915 apoptotic process
GO:0006919 activation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0007049 cell cycle
GO:0007275 multicellular organism development
GO:0007369 gastrulation
GO:0008104 protein localization
GO:0010629 negative regulation of gene expression
GO:0016567 protein ubiquitination
GO:0017148 negative regulation of translation
GO:0030178 negative regulation of Wnt signaling pathway
GO:0030308 negative regulation of cell growth
GO:0030335 positive regulation of cell migration
GO:0030822 positive regulation of cAMP catabolic process
GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0032464 positive regulation of protein homooligomerization
GO:0032880 regulation of protein localization
GO:0033137 negative regulation of peptidyl-serine phosphorylation
GO:0035556 intracellular signal transduction
GO:0040008 regulation of growth
GO:0042998 positive regulation of Golgi to plasma membrane protein transport
GO:0043065 positive regulation of apoptotic process
GO:0043473 pigmentation
GO:0043547 positive regulation of GTPase activity
GO:0048511 rhythmic process
GO:0050765 negative regulation of phagocytosis
GO:0051302 regulation of cell division
GO:0051343 positive regulation of cyclic-nucleotide phosphodiesterase activity
GO:0051726 regulation of cell cycle
GO:0051898 negative regulation of protein kinase B signaling
GO:0051901 positive regulation of mitochondrial depolarization
GO:0061099 negative regulation of protein tyrosine kinase activity
GO:0071333 cellular response to glucose stimulus
GO:0071363 cellular response to growth factor stimulus
GO:0072344 rescue of stalled ribosome
GO:1903076 regulation of protein localization to plasma membrane
GO:1903208 negative regulation of hydrogen peroxide-induced neuron death
GO:2000114 regulation of establishment of cell polarity
GO:2000543 positive regulation of gastrulation
GO:2001244 positive regulation of intrinsic apoptotic signaling pathway

Cellular Component:
GO:0001891 phagocytic cup
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0005840 ribosome
GO:0005886 plasma membrane
GO:0015935 small ribosomal subunit
GO:0016020 membrane
GO:0030425 dendrite
GO:0030496 midbody
GO:0042995 cell projection
GO:0043005 neuron projection
GO:0043025 neuronal cell body
GO:0043204 perikaryon
GO:0044297 cell body
GO:0048471 perinuclear region of cytoplasm
GO:1990630 IRE1-RACK1-PP2A complex


-  Descriptions from all associated GenBank mRNAs
  AK168349 - Mus musculus CRL-1722 L5178Y-R cDNA, RIKEN full-length enriched library, clone:I730096H10 product:guanine nucleotide binding protein, beta 2, related sequence 1, full insert sequence.
AK166868 - Mus musculus blastocyst blastocyst cDNA, RIKEN full-length enriched library, clone:I1C0019E21 product:guanine nucleotide binding protein, beta 2, related sequence 1, full insert sequence.
AK166786 - Mus musculus blastocyst blastocyst cDNA, RIKEN full-length enriched library, clone:I1C0013E14 product:guanine nucleotide binding protein, beta 2, related sequence 1, full insert sequence.
AK166800 - Mus musculus blastocyst blastocyst cDNA, RIKEN full-length enriched library, clone:I1C0014F18 product:guanine nucleotide binding protein, beta 2, related sequence 1, full insert sequence.
AK166945 - Mus musculus blastocyst blastocyst cDNA, RIKEN full-length enriched library, clone:I1C0024M22 product:guanine nucleotide binding protein, beta 2, related sequence 1, full insert sequence.
AK161234 - Mus musculus 0 day neonate head cDNA, RIKEN full-length enriched library, clone:4833440G13 product:guanine nucleotide binding protein, beta 2, related sequence 1, full insert sequence.
AK169612 - Mus musculus 2 days neonate thymus thymic cells cDNA, RIKEN full-length enriched library, clone:E430002F11 product:guanine nucleotide binding protein, beta 2, related sequence 1, full insert sequence.
AK159797 - Mus musculus osteoclast-like cell cDNA, RIKEN full-length enriched library, clone:I420031H17 product:guanine nucleotide binding protein, beta 2, related sequence 1, full insert sequence.
AK168086 - Mus musculus TIB-55 BB88 cDNA, RIKEN full-length enriched library, clone:I730056P19 product:guanine nucleotide binding protein, beta 2, related sequence 1, full insert sequence.
AK168096 - Mus musculus CRL-1722 L5178Y-R cDNA, RIKEN full-length enriched library, clone:I730057H09 product:guanine nucleotide binding protein, beta 2, related sequence 1, full insert sequence.
AK168196 - Mus musculus CRL-1722 L5178Y-R cDNA, RIKEN full-length enriched library, clone:I730064N19 product:guanine nucleotide binding protein, beta 2, related sequence 1, full insert sequence.
AK017772 - Mus musculus 8 days embryo whole body cDNA, RIKEN full-length enriched library, clone:5730515M10 product:guanine nucleotide binding protein, beta 2, related sequence 1, full insert sequence.
AK051226 - Mus musculus 12 days embryo spinal ganglion cDNA, RIKEN full-length enriched library, clone:D130019F14 product:guanine nucleotide binding protein, beta 2, related sequence 1, full insert sequence.
AK160739 - Mus musculus 13 days embryo head cDNA, RIKEN full-length enriched library, clone:3110002E16 product:guanine nucleotide binding protein, beta 2, related sequence 1, full insert sequence.
D29802 - Mus musculus p205 mRNA for G protein beta subunit like, complete cds.
X75313 - M.musculus (C57BL/6) GB-like mRNA.
AK166417 - Mus musculus mammary gland RCB-0527 Jyg-MC(B) cDNA, RIKEN full-length enriched library, clone:G930013E05 product:guanine nucleotide binding protein, beta 2, related sequence 1, full insert sequence.
AK002493 - Mus musculus adult male kidney cDNA, RIKEN full-length enriched library, clone:0610010K19 product:GUANINE NUCLEOTIDE-BINDING PROTEIN BETA SUBUNIT-LIKE PROTEIN 12.3 (P205) (RECEPTOR OF ACTIVATED PROTEIN KINASE C 1) (RACK1) (RECEPTOR FOR ACTIVATED C KINASE), full insert sequence.
BC046760 - Mus musculus guanine nucleotide binding protein (G protein), beta polypeptide 2 like 1, mRNA (cDNA clone MGC:57917 IMAGE:5693613), complete cds.
BC020322 - Mus musculus cDNA clone IMAGE:4458340.
AK163811 - Mus musculus 16 days embryo head cDNA, RIKEN full-length enriched library, clone:C130048J16 product:guanine nucleotide binding protein, beta 2, related sequence 1, full insert sequence.
AK160528 - Mus musculus ES cells cDNA, RIKEN full-length enriched library, clone:2400009B18 product:guanine nucleotide binding protein, beta 2, related sequence 1, full insert sequence.
AK012242 - Mus musculus 11 days embryo whole body cDNA, RIKEN full-length enriched library, clone:2700012L07 product:guanine nucleotide binding protein, beta 2, related sequence 1, full insert sequence.
AK166889 - Mus musculus blastocyst blastocyst cDNA, RIKEN full-length enriched library, clone:I1C0020L08 product:guanine nucleotide binding protein, beta 2, related sequence 1, full insert sequence.
BC036295 - Mus musculus guanine nucleotide binding protein (G protein), beta polypeptide 2 like 1, mRNA (cDNA clone IMAGE:3979551).
AK186349 - Mus musculus cDNA, clone:Y0G0137A21, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000020640, based on BLAT search.
LF198456 - JP 2014500723-A/5959: Polycomb-Associated Non-Coding RNAs.
MA434033 - JP 2018138019-A/5959: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu05162 - Measles

Reactome (by CSHL, EBI, and GO)

Protein P68040 (Reactome details) participates in the following event(s):

R-MMU-5626962 GNB2L1 binds IKKA:IKKB:NEMO
R-MMU-5626982 TNF-alpha:TNFR1:NSMAF binds GNB2L1
R-MMU-5626981 TNF-alpha:TNFR1:NSMAF:GNB2L1 associates with SMPD2,3
R-MMU-5357905 Regulation of TNFR1 signaling
R-MMU-5626978 TNFR1-mediated ceramide production
R-MMU-75893 TNF signaling
R-MMU-73887 Death Receptor Signalling
R-MMU-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: GBLP_MOUSE, Gnb2-rs1, Gnb2l1, NM_008143, P25388, P68040, P99049, Q3THP0, Q3THY7, Q3TKQ0, Q3TW88, Q5NCC5, Q5NCC6, Q9CSQ0, Q9ERM6, uc007ipa.1, uc007ipa.2
UCSC ID: uc007ipa.2
RefSeq Accession: NM_008143
Protein: P68040 (aka GBLP_MOUSE)
CCDS: CCDS24585.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.