Mouse Gene Nt5c1b (ENSMUST00000002456.9) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus 5'-nucleotidase, cytosolic IB (Nt5c1b), transcript variant 1, mRNA. (from RefSeq NM_027588)
Gencode Transcript: ENSMUST00000002456.9
Gencode Gene: ENSMUSG00000020622.17
Transcript (Including UTRs)
   Position: mm10 chr12:10,369,973-10,390,170 Size: 20,198 Total Exon Count: 9 Strand: +
Coding Region
   Position: mm10 chr12:10,370,063-10,390,133 Size: 20,071 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr12:10,369,973-10,390,170)mRNA (may differ from genome)Protein (573 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKB

-  Comments and Description Text from UniProtKB
  ID: 5NT1B_MOUSE
DESCRIPTION: RecName: Full=Cytosolic 5'-nucleotidase 1B; Short=cN1B; EC=3.1.3.5; AltName: Full=Autoimmune infertility-related protein; AltName: Full=Cytosolic 5'-nucleotidase IB; Short=cN-IB;
FUNCTION: Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides. Helps to regulate adenosine levels.
CATALYTIC ACTIVITY: A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate.
COFACTOR: Magnesium.
ENZYME REGULATION: Activated by ADP.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly expressed in testis and brain. Detected at lower levels in skeletal muscle, heart and kidney.
SIMILARITY: Belongs to the 5'-nucleotidase type 3 family.
SEQUENCE CAUTION: Sequence=AAK39109.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -16.5090-0.183 Picture PostScript Text
3' UTR -0.5037-0.014 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR010394 - 5-nucleotidase

Pfam Domains:
PF06189 - 5'-nucleotidase

SCOP Domains:
56784 - HAD-like

ModBase Predicted Comparative 3D Structure on Q91YE9
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
 RGDEnsembl   
 Protein Sequence    
 Alignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0000287 magnesium ion binding
GO:0008253 5'-nucleotidase activity
GO:0016787 hydrolase activity

Biological Process:
GO:0009116 nucleoside metabolic process
GO:0009117 nucleotide metabolic process
GO:0016311 dephosphorylation
GO:0046085 adenosine metabolic process

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  AK014892 - Mus musculus adult male testis cDNA, RIKEN full-length enriched library, clone:4921514H13 product:autoimmune infertility-related protein, full insert sequence.
BC060976 - Mus musculus 5'-nucleotidase, cytosolic IB, mRNA (cDNA clone MGC:74070 IMAGE:6773248), complete cds.
AF356186 - Mus musculus autoimmune infertility-related protein (Airp) mRNA, partial cds.
AJ295253 - Mus musculus mRNA for cytosolic 5' nucleotidase 1B, (5NT gene).

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu00230 - Purine metabolism
mmu00240 - Pyrimidine metabolism
mmu00760 - Nicotinate and nicotinamide metabolism
mmu01100 - Metabolic pathways

Reactome (by CSHL, EBI, and GO)

Protein Q91YE9 (Reactome details) participates in the following event(s):

R-MMU-109415 AMP + H2O => adenosine + orthophosphate [NT5C1B]
R-MMU-74259 Purine catabolism
R-MMU-8956319 Nucleobase catabolism
R-MMU-15869 Metabolism of nucleotides
R-MMU-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: 5NT1B_MOUSE, Airp, E9QNH2, NM_027588, Q91Y48, Q91YE9, uc007nap.1, uc007nap.2, uc007nap.3
UCSC ID: uc007nap.3
RefSeq Accession: NM_027588
Protein: Q91YE9 (aka 5NT1B_MOUSE or 5NIB_MOUSE)
CCDS: CCDS25810.1, CCDS79106.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.