Mouse Gene Adk (ENSMUST00000224069.1) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus adenosine kinase (Adk), transcript variant 2, mRNA. (from RefSeq NM_001243041)
Gencode Transcript: ENSMUST00000224069.1
Gencode Gene: ENSMUSG00000039197.10
Transcript (Including UTRs)
   Position: mm10 chr14:21,076,152-21,448,562 Size: 372,411 Total Exon Count: 11 Strand: +
Coding Region
   Position: mm10 chr14:21,076,308-21,447,938 Size: 371,631 Coding Exon Count: 11 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr14:21,076,152-21,448,562)mRNA (may differ from genome)Protein (345 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: ADK_MOUSE
DESCRIPTION: RecName: Full=Adenosine kinase; Short=AK; EC=2.7.1.20; AltName: Full=Adenosine 5'-phosphotransferase;
FUNCTION: ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
CATALYTIC ACTIVITY: ATP + adenosine = ADP + AMP.
COFACTOR: Binds 3 magnesium ions per subunit (By similarity).
ENZYME REGULATION: Activity is inhibited by 5-iodotubercidin and 5'-amino-5'-deoxyadenosine (By similarity).
PATHWAY: Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine: step 1/1.
SUBUNIT: Monomer (By similarity).
TISSUE SPECIFICITY: Highest expression in liver, testis, kidney and spleen. Lower expression in brain. Isoform Long is predominant in testis and kidney and isoform Short is predominant in the spleen.
SIMILARITY: Belongs to the carbohydrate kinase PfkB family.
SEQUENCE CAUTION: Sequence=AAA91649.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -67.00156-0.429 Picture PostScript Text
3' UTR -131.50624-0.211 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001805 - Adenokinase
IPR002173 - Carboh/pur_kinase_PfkB_CS
IPR011611 - PfkB

Pfam Domains:
PF00294 - pfkB family carbohydrate kinase

SCOP Domains:
53613 - Ribokinase-like

ModBase Predicted Comparative 3D Structure on P55264
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologGenome BrowserGenome BrowserNo ortholog
Gene Details    Gene Details
Gene Sorter    Gene Sorter
 RGDEnsemblEnsemblWormBaseSGD
 Protein Sequence Protein SequenceProtein Sequence 
 Alignment AlignmentAlignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004001 adenosine kinase activity
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0016773 phosphotransferase activity, alcohol group as acceptor
GO:0046872 metal ion binding
GO:0019200 carbohydrate kinase activity

Biological Process:
GO:0006166 purine ribonucleoside salvage
GO:0006169 adenosine salvage
GO:0006175 dATP biosynthetic process
GO:0010613 positive regulation of cardiac muscle hypertrophy
GO:0016310 phosphorylation
GO:0042102 positive regulation of T cell proliferation
GO:0044209 AMP salvage
GO:0044342 type B pancreatic cell proliferation
GO:0046085 adenosine metabolic process
GO:0046835 carbohydrate phosphorylation

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  AK157662 - Mus musculus activated spleen cDNA, RIKEN full-length enriched library, clone:F830230K01 product:adenosine kinase, full insert sequence.
AK139200 - Mus musculus 7 days neonate cerebellum cDNA, RIKEN full-length enriched library, clone:A730065F12 product:adenosine kinase, full insert sequence.
AK032871 - Mus musculus 12 days embryo male wolffian duct includes surrounding region cDNA, RIKEN full-length enriched library, clone:6720465D13 product:ADENOSINE KINASE (EC 2.7.1.20) (FRAGMENT) homolog [Cricetulus griseus], full insert sequence.
AY540996 - Mus musculus adenosine kinase long isoform (Adk) mRNA, complete cds.
AK151863 - Mus musculus bone marrow macrophage cDNA, RIKEN full-length enriched library, clone:I830041D18 product:adenosine kinase, full insert sequence.
AK160264 - Mus musculus 11 days pregnant adult female ovary and uterus cDNA, RIKEN full-length enriched library, clone:5033405D03 product:adenosine kinase, full insert sequence.
AY540997 - Mus musculus adenosine kinase short isoform (Adk) mRNA, complete cds.
AK050135 - Mus musculus adult male liver tumor cDNA, RIKEN full-length enriched library, clone:C730019E20 product:SIMILAR TO ADENOSIN KINASE homolog [Mus musculus], full insert sequence.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu00230 - Purine metabolism
mmu01100 - Metabolic pathways

Reactome (by CSHL, EBI, and GO)

Protein P55264 (Reactome details) participates in the following event(s):

R-MMU-109624 (2'-deoxy)adenosine + ATP => (d)AMP + ADP (ADK)
R-MMU-74217 Purine salvage
R-MMU-8956321 Nucleotide salvage
R-MMU-15869 Metabolism of nucleotides
R-MMU-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: ADK_MOUSE, NM_001243041, P55264, Q6JAM3, Q91VJ3, uc007sle.1, uc007sle.2, uc007sle.3
UCSC ID: uc007sle.3
RefSeq Accession: NM_001243041
Protein: P55264 (aka ADK_MOUSE)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.