Mouse Gene Kng1 (ENSMUST00000039492.13) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus kininogen 1 (Kng1), transcript variant 2, mRNA. (from RefSeq NM_023125)
Gencode Transcript: ENSMUST00000039492.13
Gencode Gene: ENSMUSG00000022875.18
Transcript (Including UTRs)
   Position: mm10 chr16:23,058,250-23,082,078 Size: 23,829 Total Exon Count: 11 Strand: +
Coding Region
   Position: mm10 chr16:23,058,444-23,081,576 Size: 23,133 Coding Exon Count: 11 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr16:23,058,250-23,082,078)mRNA (may differ from genome)Protein (432 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: KNG1_MOUSE
DESCRIPTION: RecName: Full=Kininogen-1; Contains: RecName: Full=Kininogen-1 heavy chain; Contains: RecName: Full=Bradykinin; Contains: RecName: Full=Kininogen-1 light chain; Flags: Precursor;
FUNCTION: (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin- induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW- kininogen is in contrast to HMW-kininogen not involved in blood clotting (By similarity).
SUBUNIT: Isoform LMW interacts with CRISP3.
SUBCELLULAR LOCATION: Secreted, extracellular space.
TISSUE SPECIFICITY: Plasma.
PTM: Bradykinin is released from kininogen by plasma kallikrein.
PTM: Phosphorylation sites are present in the extracellular medium (By similarity).
SIMILARITY: Contains 3 cystatin kininogen-type domains.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -71.10194-0.366 Picture PostScript Text
3' UTR -156.20502-0.311 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002395 - Kininogen
IPR000010 - Prot_inh_cystat
IPR018073 - Prot_inh_cystat_CS

Pfam Domains:
PF00031 - Cystatin domain
PF00666 - Cathelicidin

SCOP Domains:
54403 - Cystatin/monellin

ModBase Predicted Comparative 3D Structure on O08677
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologGenome BrowserNo orthologNo ortholog
Gene Details     
Gene Sorter     
GenBankRGD Ensembl  
Protein SequenceProtein Sequence Protein Sequence  
AlignmentAlignment Alignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004869 cysteine-type endopeptidase inhibitor activity
GO:0005102 receptor binding
GO:0030414 peptidase inhibitor activity

Biological Process:
GO:0006954 inflammatory response
GO:0007204 positive regulation of cytosolic calcium ion concentration
GO:0007596 blood coagulation
GO:0007599 hemostasis
GO:0010466 negative regulation of peptidase activity
GO:0010951 negative regulation of endopeptidase activity
GO:0030195 negative regulation of blood coagulation
GO:0042311 vasodilation
GO:0050880 regulation of blood vessel size

Cellular Component:
GO:0005576 extracellular region
GO:0005615 extracellular space
GO:0005623 cell
GO:0031012 extracellular matrix
GO:0072562 blood microparticle


-  Descriptions from all associated GenBank mRNAs
  AK005547 - Mus musculus adult female placenta cDNA, RIKEN full-length enriched library, clone:1600027I01 product:kininogen, full insert sequence.
BC108936 - Mus musculus kininogen 1, mRNA (cDNA clone MGC:123437 IMAGE:40039851), complete cds.
BC018158 - Mus musculus kininogen 1, mRNA (cDNA clone MGC:25815 IMAGE:4162960), complete cds.
AY660571 - Mus musculus high molecular weight kininogen I isoform DeltaD5 mRNA, complete cds, alternatively spliced.
AY462058 - Mus musculus HMW kininogen-I variant mRNA, complete cds, alternatively spliced.
D84435 - Mus sp. mRNA for HMW prekininogen, complete cds.
D84415 - Mus sp. mRNA for LMW prekininogen, complete cds.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu04610 - Complement and coagulation cascades

BioCarta from NCI Cancer Genome Anatomy Project
m_intrinsicPathway - Intrinsic Prothrombin Activation Pathway

Reactome (by CSHL, EBI, and GO)

Protein O08677 (Reactome details) participates in the following event(s):

R-MMU-374331 Bradykinin receptors B1 and B2 bind to bradykinin
R-MMU-158311 kallikrein:kininogen:C1q binding protein tetramer -> kallikrein + activated kininogen:C1q binding protein tetramer + bradykinin
R-MMU-158354 kininogen + C1q binding protein tetramer -> kininogen:C1q binding protein tetramer
R-MMU-158218 prekallikrein + kininogen:C1q binding protein tetramer -> prekallikrein:kininogen:C1q binding protein tetramer
R-MMU-749452 The Ligand:GPCR:Gq complex dissociates
R-MMU-749454 The Ligand:GPCR:Gi complex dissociates
R-MMU-481007 Exocytosis of platelet alpha granule contents
R-MMU-158251 prekallikrein:kininogen:C1q binding protein tetramer -> kallikrein:kininogen:C1q binding protein tetramer
R-MMU-749448 Liganded Gq-activating GPCRs bind inactive heterotrimeric Gq
R-MMU-749456 Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi
R-MMU-8952289 FAM20C phosphorylates FAM20C substrates
R-MMU-158313 factor XII -> factor XIIa
R-MMU-379048 Liganded Gq/11-activating GPCRs act as GEFs for Gq/11
R-MMU-380073 Liganded Gi-activating GPCR acts as a GEF for Gi
R-MMU-375276 Peptide ligand-binding receptors
R-MMU-140837 Intrinsic Pathway of Fibrin Clot Formation
R-MMU-416476 G alpha (q) signalling events
R-MMU-418594 G alpha (i) signalling events
R-MMU-373076 Class A/1 (Rhodopsin-like receptors)
R-MMU-140877 Formation of Fibrin Clot (Clotting Cascade)
R-MMU-114608 Platelet degranulation
R-MMU-388396 GPCR downstream signalling
R-MMU-500792 GPCR ligand binding
R-MMU-109582 Hemostasis
R-MMU-76005 Response to elevated platelet cytosolic Ca2+
R-MMU-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-MMU-8957275 Post-translational protein phosphorylation
R-MMU-372790 Signaling by GPCR
R-MMU-76002 Platelet activation, signaling and aggregation
R-MMU-392499 Metabolism of proteins
R-MMU-597592 Post-translational protein modification
R-MMU-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: Kng, KNG1_MOUSE, NM_023125, O08676, O08677, Q32MX7, Q6S9I1, Q91XK5, uc007ytc.1, uc007ytc.2
UCSC ID: uc007ytc.2
RefSeq Accession: NM_023125
Protein: O08677 (aka KNG1_MOUSE)
CCDS: CCDS28073.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.