Mouse Gene Myl12a (ENSMUST00000148960.7) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus myosin, light chain 12A, regulatory, non-sarcomeric (Myl12a), transcript variant 1, mRNA. (from RefSeq NM_026064)
Gencode Transcript: ENSMUST00000148960.7
Gencode Gene: ENSMUSG00000024048.15
Transcript (Including UTRs)
   Position: mm10 chr17:70,993,656-71,002,554 Size: 8,899 Total Exon Count: 4 Strand: -
Coding Region
   Position: mm10 chr17:70,994,612-70,996,880 Size: 2,269 Coding Exon Count: 3 

Page IndexSequence and LinksPrimersCTDRNA StructureProtein Structure
Other SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther NamesModel Information
Methods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:70,993,656-71,002,554)mRNA (may differ from genome)Protein (172 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKBWikipedia

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -195.00462-0.422 Picture PostScript Text
3' UTR -246.40956-0.258 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR018248 - EF-hand
IPR011992 - EF-hand-like_dom
IPR018247 - EF_Hand_1_Ca_BS
IPR018249 - EF_HAND_2
IPR002048 - EF_hand_Ca-bd

Pfam Domains:
PF00036 - EF hand
PF13202 - EF hand
PF13405 - EF-hand domain
PF13499 - EF-hand domain pair
PF13833 - EF-hand domain pair

SCOP Domains:
109998 - Triger factor/SurA peptide-binding domain-like
47473 - EF-hand

ModBase Predicted Comparative 3D Structure on Q6ZWQ9
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
      
      
 RGD    
 Protein Sequence    
 Alignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005509 calcium ion binding
GO:0005515 protein binding
GO:0035254 glutamate receptor binding

Biological Process:
GO:0008360 regulation of cell shape
GO:0072659 protein localization to plasma membrane

Cellular Component:
GO:0001725 stress fiber
GO:0016460 myosin II complex
GO:0030018 Z disc
GO:0032991 macromolecular complex
GO:0099738 cell cortex region


-  Descriptions from all associated GenBank mRNAs
  AK013776 - Mus musculus adult male hippocampus cDNA, RIKEN full-length enriched library, clone:2900073G15 product:myosin regulatory light chain A, smooth muscle homolog [Rattus norvegicus], full insert sequence.
BC010265 - Mus musculus RIKEN cDNA 2900073G15 gene, mRNA (cDNA clone MGC:8089 IMAGE:3588394), complete cds.
AK018348 - Mus musculus 12 days embryo female ovary cDNA, RIKEN full-length enriched library, clone:6620401G15 product:myosin regulatory light chain A, smooth muscle homolog [Rattus norvegicus], full insert sequence.
BC020282 - Mus musculus RIKEN cDNA 2900073G15 gene, mRNA (cDNA clone IMAGE:3153484).
BC029180 - Mus musculus RIKEN cDNA 2900073G15 gene, mRNA (cDNA clone MGC:35677 IMAGE:2599135), complete cds.
BC006719 - Mus musculus RIKEN cDNA 2900073G15 gene, mRNA (cDNA clone IMAGE:3711075), partial cds.
BC089050 - Mus musculus cDNA clone IMAGE:5328490, **** WARNING: chimeric clone ****.
AK045160 - Mus musculus 9.5 days embryo parthenogenote cDNA, RIKEN full-length enriched library, clone:B130042B20 product:myosin regulatory light chain A, smooth muscle homolog [Rattus norvegicus], full insert sequence.
AK168016 - Mus musculus CRL-1722 L5178Y-R cDNA, RIKEN full-length enriched library, clone:I730047D20 product:Myosin regulatory light chain 2-A, smooth muscle isoform (Myosin RLC-A) homolog [Rattus norvegicus], full insert sequence.
AK159151 - Mus musculus osteoclast-like cell cDNA, RIKEN full-length enriched library, clone:I420005I07 product:Myosin regulatory light chain 2-A, smooth muscle isoform (Myosin RLC-A) homolog [Rattus norvegicus], full insert sequence.
AK167553 - Mus musculus 14 days pregnant adult female placenta cDNA, RIKEN full-length enriched library, clone:I530020L12 product:Myosin regulatory light chain 2-A, smooth muscle isoform (Myosin RLC-A) homolog [Rattus norvegicus], full insert sequence.
AK151265 - Mus musculus bone marrow macrophage cDNA, RIKEN full-length enriched library, clone:I830027H03 product:Myosin regulatory light chain 2-A, smooth muscle isoform (Myosin RLC-A) homolog [Rattus norvegicus], full insert sequence.
AK028020 - Mus musculus 18-day embryo whole body cDNA, RIKEN full-length enriched library, clone:1190017I13 product:myosin regulatory light chain A, smooth muscle homolog [Rattus norvegicus], full insert sequence.
LF249244 - JP 2014500723-A/56747: Polycomb-Associated Non-Coding RNAs.
MA484821 - JP 2018138019-A/56747: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu04510 - Focal adhesion
mmu04530 - Tight junction
mmu04670 - Leukocyte transendothelial migration
mmu04810 - Regulation of actin cytoskeleton

Reactome (by CSHL, EBI, and GO)

Protein Q6ZWQ9 (Reactome details) participates in the following event(s):

R-MMU-3928640 PAKs phosphorylate MLC
R-MMU-445700 Myosin Binds ATP
R-MMU-445704 Calcium Binds Caldesmon
R-MMU-445705 Release Of ADP From Myosin
R-MMU-445699 ATP Hydrolysis By Myosin
R-MMU-3928664 Ephrin signaling
R-MMU-2682334 EPH-Ephrin signaling
R-MMU-445355 Smooth Muscle Contraction
R-MMU-422475 Axon guidance
R-MMU-397014 Muscle contraction
R-MMU-1266738 Developmental Biology

-  Other Names for This Gene
  Alternate Gene Symbols: mCG_5400, NM_026064, Q6ZWQ9, Q6ZWQ9_MOUSE, uc008dlw.1, uc008dlw.2
UCSC ID: uc008dlw.2
RefSeq Accession: NM_026064
Protein: Q6ZWQ9 CCDS: CCDS28955.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.