Mouse Gene Spink1 (ENSMUST00000025381.3) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus serine peptidase inhibitor, Kazal type 1 (Spink1), mRNA. (from RefSeq NM_009258)
Gencode Transcript: ENSMUST00000025381.3
Gencode Gene: ENSMUSG00000024503.3
Transcript (Including UTRs)
   Position: mm10 chr18:43,728,082-43,737,557 Size: 9,476 Total Exon Count: 4 Strand: -
Coding Region
   Position: mm10 chr18:43,728,160-43,737,149 Size: 8,990 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther NamesModel Information
Methods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr18:43,728,082-43,737,557)mRNA (may differ from genome)Protein (80 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: ISK3_MOUSE
DESCRIPTION: RecName: Full=Serine protease inhibitor Kazal-type 3; AltName: Full=P12; AltName: Full=Prostatic secretory glycoprotein; Flags: Precursor;
FUNCTION: Serine protease inhibitor which exhibits anti-trypsin activity. Inhibits the uptake of calcium by spermatozoa.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: In the genital tract, expressed only in male accessory glands including seminal vesicle, coagulating gland and prostate.
DEVELOPMENTAL STAGE: Not expressed during prepubertal stages. Expression coincides with maturation.
INDUCTION: By androgens in adult male sex accessory glands but expressed constitutively in pancreas.
SIMILARITY: Contains 1 Kazal-like domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -112.60408-0.276 Picture PostScript Text
3' UTR -11.1078-0.142 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002350 - Prot_inh_Kazal
IPR001239 - Prot_inh_Kazal-m

Pfam Domains:
PF00050 - Kazal-type serine protease inhibitor domain
PF07648 - Kazal-type serine protease inhibitor domain

SCOP Domains:
100895 - Kazal-type serine protease inhibitors

ModBase Predicted Comparative 3D Structure on P09036
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologGenome BrowserNo orthologNo ortholog
      
      
  EnsemblEnsembl  
   Protein Sequence  
   Alignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004867 serine-type endopeptidase inhibitor activity
GO:0030414 peptidase inhibitor activity

Biological Process:
GO:0010466 negative regulation of peptidase activity
GO:0010751 negative regulation of nitric oxide mediated signal transduction
GO:0048240 sperm capacitation
GO:0050732 negative regulation of peptidyl-tyrosine phosphorylation
GO:0060046 regulation of acrosome reaction
GO:0090281 negative regulation of calcium ion import
GO:1900004 negative regulation of serine-type endopeptidase activity
GO:2001256 regulation of store-operated calcium entry

Cellular Component:
GO:0001669 acrosomal vesicle
GO:0005576 extracellular region
GO:0005615 extracellular space


-  Descriptions from all associated GenBank mRNAs
  BC066214 - Mus musculus cDNA clone IMAGE:6431230, **** WARNING: chimeric clone ****.
BC086887 - Mus musculus serine peptidase inhibitor, Kazal type 3, mRNA (cDNA clone MGC:107555 IMAGE:6775994), complete cds.
X06342 - Mouse mRNA for prostatic secretory glycoprotein (p12) homologous to secretory protease inhibitor.
AK007985 - Mus musculus 10 day old male pancreas cDNA, RIKEN full-length enriched library, clone:1810074D19 product:serine protease inhibitor, Kazal type 3, full insert sequence.
AK007841 - Mus musculus 10 day old male pancreas cDNA, RIKEN full-length enriched library, clone:1810049G06 product:serine protease inhibitor, Kazal type 3, full insert sequence.

-  Other Names for This Gene
  Alternate Gene Symbols: ISK3_MOUSE, NM_009258, P09036, Q5M9M3, Spink3, uc008eul.1, uc008eul.2, uc008eul.3
UCSC ID: uc008eul.3
RefSeq Accession: NM_009258
Protein: P09036 (aka ISK3_MOUSE)
CCDS: CCDS37803.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.