Mouse Gene G6pc2 (ENSMUST00000005364.11) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus glucose-6-phosphatase, catalytic, 2 (G6pc2), transcript variant 1, mRNA. (from RefSeq NM_021331)
RefSeq Summary (NM_021331): This gene encodes an enzyme that belongs to the glucose-6-phosphatase catalytic subunit family. Members of this family catalyze the hydrolysis of glucose-6-phosphate, the terminal step in gluconeogenic and glycogenolytic pathways, to release glucose into the bloodstream. The family member encoded by this gene is found specifically in pancreatic islets but has not been shown to have phosphotransferase or phosphatase activity exhibited by a similar liver enzyme. The non-obese diabetic (NOD) mouse is a model for human type 1 diabetes, an autoimmune disease in which T lymphocytes attack and destroy insulin-producing pancreatic beta cells. In NOD mice, the protein encoded by this gene is a major target of cell-mediated autoimmunity. Variations in the human and mouse genes are associated with lower fasting plasma glucose levels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014].
Gencode Transcript: ENSMUST00000005364.11
Gencode Gene: ENSMUSG00000005232.11
Transcript (Including UTRs)
   Position: mm10 chr2:69,219,971-69,227,841 Size: 7,871 Total Exon Count: 5 Strand: +
Coding Region
   Position: mm10 chr2:69,220,033-69,227,079 Size: 7,047 Coding Exon Count: 5 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:69,219,971-69,227,841)mRNA (may differ from genome)Protein (355 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: G6PC2_MOUSE
DESCRIPTION: RecName: Full=Glucose-6-phosphatase 2; Short=G-6-Pase 2; Short=G6Pase 2; EC=3.1.3.9; AltName: Full=Islet-specific glucose-6-phosphatase catalytic subunit-related protein;
FUNCTION: May hydrolyze glucose-6-phosphate to glucose in the endoplasmic reticulum. May be responsible for glucose production through glycogenolysis and gluconeogenesis (By similarity).
CATALYTIC ACTIVITY: D-glucose 6-phosphate + H(2)O = D-glucose + phosphate.
PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity).
TISSUE SPECIFICITY: Specifically expressed in pancreatic islet cells, in particular those of beta-cell origin. Not detected in testis, kidney, muscle, liver, lung, spleen, brain, pituitary, gastric fundus or heart.
DEVELOPMENTAL STAGE: Initial onset of expression in the pancreas is at E12 and prominent expression is detected at E14.
INDUCTION: Up-regulated in islet cells cultured in hyperglycemic concentrations of glucose.
PTM: N-glycosylated; the non-glycosylated form is more unstable and is degraded through the proteasome (By similarity).
DISRUPTION PHENOTYPE: Mice are no overt anatomical or behavioral phenotype but display a mild metabolic phenotype. Upon fasting those mice exhibit a significant decrease in blood glucose and triacylglycerol compared to wild type mice.
MISCELLANEOUS: G6pc2 is an autoantigen which is the natural target of a prevalent T-cell population causing insulin-dependent diabetes mellitus through destruction of pancreatic beta cells.
SIMILARITY: Belongs to the glucose-6-phosphatase family.
SEQUENCE CAUTION: Sequence=AAI11906.1; Type=Erroneous translation; Note=Wrong choice of CDS;

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -17.4062-0.281 Picture PostScript Text
3' UTR -184.70762-0.242 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR016275 - Glucose-6-phosphatase
IPR016118 - P_Acid_Pase/Cl_peroxidase_N
IPR000326 - P_Acid_Pase_2/haloperoxidase

Pfam Domains:
PF01569 - PAP2 superfamily

SCOP Domains:
48317 - Acid phosphatase/Vanadium-dependent haloperoxidase

ModBase Predicted Comparative 3D Structure on Q9Z186
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologGenome BrowserNo orthologNo ortholog
      
      
   Ensembl  
   Protein Sequence  
   Alignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0016787 hydrolase activity
GO:0004346 glucose-6-phosphatase activity

Biological Process:
GO:0006094 gluconeogenesis
GO:0042593 glucose homeostasis
GO:0050796 regulation of insulin secretion
GO:0051156 glucose 6-phosphate metabolic process

Cellular Component:
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0030176 integral component of endoplasmic reticulum membrane


-  Descriptions from all associated GenBank mRNAs
  AK148450 - Mus musculus adult pancreas islet cells cDNA, RIKEN full-length enriched library, clone:C820002B04 product:glucose-6-phosphatase, catalytic, 2, full insert sequence.
Z47787 - M.musculus mRNA for glucose-6-phosphatase.
BC111905 - Mus musculus glucose-6-phosphatase, catalytic, 2, mRNA (cDNA clone IMAGE:40039118), complete cds.
AK148465 - Mus musculus adult pancreas islet cells cDNA, RIKEN full-length enriched library, clone:C820005N03 product:glucose-6-phosphatase, catalytic, 2, full insert sequence.
BC141558 - Synthetic construct Mus musculus clone IMAGE:100014526, MGC:175397 glucose-6-phosphatase, catalytic, 2 (G6pc2) mRNA, encodes complete protein.
BC148698 - Synthetic construct Mus musculus clone IMAGE:100015702, MGC:183404 glucose-6-phosphatase, catalytic, 2 (G6pc2) mRNA, encodes complete protein.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9Z186 (Reactome details) participates in the following event(s):

R-MMU-3266566 G6PC2 hydrolyzes glucose 6-phosphate to form glucose and orthophosphate (islet)
R-MMU-70263 Gluconeogenesis
R-MMU-70326 Glucose metabolism
R-MMU-71387 Metabolism of carbohydrates
R-MMU-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: A2AUN2, G6PC2_MOUSE, Igrp, NM_021331, Q2M2M7, Q9Z186, uc008jxy.1, uc008jxy.2, uc008jxy.3
UCSC ID: uc008jxy.3
RefSeq Accession: NM_021331
Protein: Q9Z186 (aka G6PC2_MOUSE)
CCDS: CCDS16089.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.