Mouse Gene Ndufs3 (ENSMUST00000005647.3) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus NADH:ubiquinone oxidoreductase core subunit S3 (Ndufs3), mRNA. (from RefSeq NM_026688)
Gencode Transcript: ENSMUST00000005647.3
Gencode Gene: ENSMUSG00000005510.9
Transcript (Including UTRs)
   Position: mm10 chr2:90,894,636-90,904,827 Size: 10,192 Total Exon Count: 7 Strand: -
Coding Region
   Position: mm10 chr2:90,894,710-90,904,647 Size: 9,938 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr2:90,894,636-90,904,827)mRNA (may differ from genome)Protein (263 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial; EC=; EC=; AltName: Full=Complex I-30kD; Short=CI-30kD; AltName: Full=NADH-ubiquinone oxidoreductase 30 kDa subunit; Flags: Precursor;
FUNCTION: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).
CATALYTIC ACTIVITY: NADH + ubiquinone = NAD(+) + ubiquinol.
CATALYTIC ACTIVITY: NADH + acceptor = NAD(+) + reduced acceptor.
SUBUNIT: Complex I is composed of 45 different subunits (By similarity). Interacts with NDUFAF3 (By similarity).
SUBCELLULAR LOCATION: Mitochondrion inner membrane (By similarity). Note=Matrix and cytoplasmic side of the mitochondrial inner membrane (By similarity).
SIMILARITY: Belongs to the complex I 30 kDa subunit family.

-  Primer design for this transcript

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -60.30180-0.335 Picture PostScript Text
3' UTR -9.4074-0.127 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR010218 - NADH_DH_suC
IPR001268 - NADH_UbQ_OxRdtase_30kDa_su
IPR020396 - NADH_UbQ_OxRdtase_CS

Pfam Domains:
PF00329 - Respiratory-chain NADH dehydrogenase, 30 Kd subunit

SCOP Domains:
51658 - Xylose isomerase-like
51445 - (Trans)glycosidases
143243 - Nqo5-like

ModBase Predicted Comparative 3D Structure on Q9DCT2
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologGenome BrowserNo orthologNo ortholog
Gene Details     
Gene Sorter     
Protein SequenceProtein Sequence Protein Sequence  
AlignmentAlignment Alignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003954 NADH dehydrogenase activity
GO:0008137 NADH dehydrogenase (ubiquinone) activity
GO:0016491 oxidoreductase activity
GO:0016651 oxidoreductase activity, acting on NAD(P)H

Biological Process:
GO:0030308 negative regulation of cell growth
GO:0045333 cellular respiration
GO:0055114 oxidation-reduction process
GO:0072593 reactive oxygen species metabolic process
GO:2001243 negative regulation of intrinsic apoptotic signaling pathway

Cellular Component:
GO:0005739 mitochondrion
GO:0005743 mitochondrial inner membrane
GO:0005747 mitochondrial respiratory chain complex I
GO:0016020 membrane
GO:0016604 nuclear body
GO:0031966 mitochondrial membrane
GO:0043209 myelin sheath
GO:0070469 respiratory chain

-  Descriptions from all associated GenBank mRNAs
  AK207511 - Mus musculus cDNA, clone:Y2G0108E05, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000005647, based on BLAT search.
AK085775 - Mus musculus 16 days neonate heart cDNA, RIKEN full-length enriched library, clone:D830007F24 product:unclassifiable, full insert sequence.
BC027270 - Mus musculus NADH dehydrogenase (ubiquinone) Fe-S protein 3, mRNA (cDNA clone MGC:27855 IMAGE:3491580), complete cds.
AK088337 - Mus musculus 2 days neonate thymus thymic cells cDNA, RIKEN full-length enriched library, clone:E430013F21 product:NADH dehydrogenase (ubiquinone) Fe-S protein 3 (30kD) (NADH-coenzyme Q reductase), full insert sequence.
AK160888 - Mus musculus 12 days embryo embryonic body below diaphragm region cDNA, RIKEN full-length enriched library, clone:3732413B11 product:NADH dehydrogenase (ubiquinone) Fe-S protein 3, full insert sequence.
AK002501 - Mus musculus adult male kidney cDNA, RIKEN full-length enriched library, clone:0610010M09 product:NADH-UBIQUINONE OXIDOREDUCTASE 30 KDA SUBUNIT, MITOCHONDRIAL PRECURSOR (EC (EC (COMPLEX I-30KD) (CI-30KD) homolog [Homo sapiens], full insert sequence.
BC119267 - Mus musculus NADH dehydrogenase (ubiquinone) Fe-S protein 3, mRNA (cDNA clone MGC:155583 IMAGE:8734016), complete cds.
BC119269 - Mus musculus NADH dehydrogenase (ubiquinone) Fe-S protein 3, mRNA (cDNA clone MGC:155585 IMAGE:8734018), complete cds.
AK207804 - Mus musculus cDNA, clone:Y2G0109C24, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000005647, based on BLAT search.
AK187309 - Mus musculus cDNA, clone:Y0G0141C16, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000005647, based on BLAT search.
AK206263 - Mus musculus cDNA, clone:Y2G0104E12, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000005647, based on BLAT search.
AK182945 - Mus musculus cDNA, clone:Y0G0124D22, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000005647, based on BLAT search.
AK185633 - Mus musculus cDNA, clone:Y0G0134L15, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000005647, based on BLAT search.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu00190 - Oxidative phosphorylation
mmu01100 - Metabolic pathways
mmu05010 - Alzheimer's disease
mmu05012 - Parkinson's disease
mmu05016 - Huntington's disease

BioCyc Knowledge Library
PWY-3781 - aerobic respiration -- electron donor II
PWY0-1334 - NADH to cytochrome bd oxidase electron transfer
PWY0-1335 - NADH to cytochrome bo oxidase electron transfer

Reactome (by CSHL, EBI, and GO)

Protein Q9DCT2 (Reactome details) participates in the following event(s):

R-MMU-6800868 NDUF subunits bind to form the IP subcomplex
R-MMU-6799203 IP subcomplex binds NDUFAF3, NDUFAF4, TIMMDC1 to form Intermediate 1
R-MMU-6799178 Intermediate 1 binds HP subcomplex to form Intermediate 2
R-MMU-6799191 Intermediate 2 binds MT-ND1:NDUFAF5:NDUFAF6 to form a 315kDa subcomplex
R-MMU-6799202 The 315kDa subcomplex binds the 370kDa subcomplex to form the 550kDa complex
R-MMU-6799197 ND4, ND5 bind the 550kDa complex to form the 815kDa complex
R-MMU-6799196 The MCIA complex, NDUFAF2-7 all dissociate from the 980kDa complex, resulting in Complex I
R-MMU-6799179 Peripheral arm subunits bind the 815kDa complex to form a 980kDa complex
R-MMU-163217 Complex I oxidises NADH to NAD+, reduces CoQ to QH2
R-MMU-6799198 Complex I biogenesis
R-MMU-611105 Respiratory electron transport
R-MMU-163200 Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins.
R-MMU-1428517 The citric acid (TCA) cycle and respiratory electron transport
R-MMU-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: NDUS3_MOUSE, NM_026688, Q8BTZ3, Q8R073, Q9DCT2, uc008ktr.1, uc008ktr.2
UCSC ID: uc008ktr.2
RefSeq Accession: NM_026688
Protein: Q9DCT2 (aka NDUS3_MOUSE)
CCDS: CCDS16418.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.