Mouse Gene Tomm34 (ENSMUST00000018466.3) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus translocase of outer mitochondrial membrane 34 (Tomm34), transcript variant Tom34b, mRNA. (from RefSeq NM_025996)
Gencode Transcript: ENSMUST00000018466.3
Gencode Gene: ENSMUSG00000018322.10
Transcript (Including UTRs)
   Position: mm10 chr2:164,053,540-164,071,102 Size: 17,563 Total Exon Count: 7 Strand: -
Coding Region
   Position: mm10 chr2:164,054,403-164,070,593 Size: 16,191 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther NamesModel Information
Methods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:164,053,540-164,071,102)mRNA (may differ from genome)Protein (309 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: TOM34_MOUSE
DESCRIPTION: RecName: Full=Mitochondrial import receptor subunit TOM34; AltName: Full=Translocase of outer membrane 34 kDa subunit;
FUNCTION: Plays a role in the import of cytosolically synthesized preproteins into mitochondria. Binds the mature portion of precursor proteins. Interacts with cellular components, and possesses weak ATPase activity. May be a chaperone-like protein that helps to keep newly synthesized precursors in an unfolded import compatible state (By similarity).
SUBUNIT: Interacts with HSP90A, VCP, ATP6M, KIAA0665, AMPK, and DMAP1 through its TPR repeat (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side (By similarity).
TISSUE SPECIFICITY: Isoform 1 is ubiquitously expressed while isoform 2 is expressed only in mature testicular germ cells. Isoform 1 is expressed in all testicular cells. Isoform 2 is highly expressed in early to late pachytene cells but expression is significantly decreased in round spermatid cells.
DEVELOPMENTAL STAGE: Isoform 2 is hardly detectable in testis at 1 week post partum but expression gradually increases for 2 to 4 weeks post partum. Isoform 1 is detected during this period but its expression does not change.
DISRUPTION PHENOTYPE: Mice are viable, grow normally and both males and females are fertile. There is no effect on spermatogenesis or protein import into mitochondria.
SIMILARITY: Belongs to the Tom34 family.
SIMILARITY: Contains 6 TPR repeats.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -211.80509-0.416 Picture PostScript Text
3' UTR -272.30863-0.316 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001440 - TPR-1
IPR013026 - TPR-contain_dom
IPR011990 - TPR-like_helical
IPR019734 - TPR_repeat

Pfam Domains:
PF00515 - Tetratricopeptide repeat
PF07719 - Tetratricopeptide repeat
PF13181 - Tetratricopeptide repeat

SCOP Domains:
81901 - HCP-like
48439 - Protein prenylyltransferase
48452 - TPR-like
116846 - MIT domain

ModBase Predicted Comparative 3D Structure on Q9CYG7
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
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Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0031072 heat shock protein binding

Biological Process:
GO:0006626 protein targeting to mitochondrion

Cellular Component:
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005741 mitochondrial outer membrane
GO:0005829 cytosol
GO:0016020 membrane


-  Descriptions from all associated GenBank mRNAs
  BC018278 - Mus musculus translocase of outer mitochondrial membrane 34, mRNA (cDNA clone MGC:18781 IMAGE:4187356), complete cds.
AK011786 - Mus musculus 10 days embryo whole body cDNA, RIKEN full-length enriched library, clone:2610100K07 product:DJ1069P2.2 (OUTER MITOCHONDRIAL MEMBRANE TRANSLOCASE HTOM34P) (SIMILAR TO TRANSLOCASE OF OUTER MITOCHONDRIAL MEMBRANE 34) (HYPOTHETICAL 34.6 KDA PROTEIN) (UNKNOWN) (PROTEIN FOR MGC:3515) homolog [Homo sapiens], full insert sequence.
AK017699 - Mus musculus 8 days embryo whole body cDNA, RIKEN full-length enriched library, clone:5730476E06 product:DJ1069P2.2 (OUTER MITOCHONDRIAL MEMBRANE TRANSLOCASE HTOM34P) (SIMILAR TO TRANSLOCASE OF OUTER MITOCHONDRIAL MEMBRANE 34) (HYPOTHETICAL 34.6 KDA PROTEIN) (UNKNOWN) (PROTEIN FOR MGC:3515) homolog [Homo sapiens], full insert sequence.
AK144450 - Mus musculus 0 day neonate cortex cDNA, RIKEN full-length enriched library, clone:G630070F02 product:translocase of outer mitochondrial membrane 34, full insert sequence.
AK168329 - Mus musculus CRL-1722 L5178Y-R cDNA, RIKEN full-length enriched library, clone:I730091B05 product:translocase of outer mitochondrial membrane 34, full insert sequence.
AK075873 - Mus musculus adult male tongue cDNA, RIKEN full-length enriched library, clone:2310009L17 product:DJ1069P2.2 (OUTER MITOCHONDRIAL MEMBRANE TRANSLOCASE HTOM34P) (SIMILAR TO TRANSLOCASE OF OUTER MITOCHONDRIAL MEMBRANE 34) (HYPOTHETICAL 34.6 KDA PROTEIN) (UNKNOWN) (PROTEIN FOR MGC:3515) homolog [Homo sapiens], full insert sequence.
LF193747 - JP 2014500723-A/1250: Polycomb-Associated Non-Coding RNAs.
LF265388 - JP 2014500723-A/72891: Polycomb-Associated Non-Coding RNAs.
LF265389 - JP 2014500723-A/72892: Polycomb-Associated Non-Coding RNAs.
MA429324 - JP 2018138019-A/1250: Polycomb-Associated Non-Coding RNAs.
MA500965 - JP 2018138019-A/72891: Polycomb-Associated Non-Coding RNAs.
MA500966 - JP 2018138019-A/72892: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: A2A5N5, A2A5N6, NM_025996, Q3THD0, Q812F1, Q812F2, Q9CYG7, Q9D059, TOM34_MOUSE, uc008ntr.1, uc008ntr.2
UCSC ID: uc008ntr.2
RefSeq Accession: NM_025996
Protein: Q9CYG7 (aka TOM34_MOUSE)
CCDS: CCDS17020.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.