ID:DPH5_MOUSE DESCRIPTION: RecName: Full=Diphthine synthase; EC=2.1.1.98; AltName: Full=Diphthamide biosynthesis methyltransferase; FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF- 2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis. CATALYTIC ACTIVITY: 3 S-adenosyl-L-methionine + 2-(3-carboxy-3- aminopropyl)-L-histidine = 3 S-adenosyl-L-homocysteine + 2-(3- carboxy-3-(trimethylammonio)propyl)-L-histidine. PATHWAY: Protein modification; peptidyl-diphthamide biosynthesis. SIMILARITY: Belongs to the diphthine synthase family.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9CWQ0
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AK045127 - Mus musculus 9.5 days embryo parthenogenote cDNA, RIKEN full-length enriched library, clone:B130038I08 product:weakly similar to PUTATIVE DIPHTHINE SYNTHASE [Schizosaccharomyces pombe], full insert sequence. AK046753 - Mus musculus 4 days neonate thymus cDNA, RIKEN full-length enriched library, clone:B630008G01 product:Weakly similar to putative diphthine synthase, full insert sequence. AK034652 - Mus musculus 12 days embryo embryonic body between diaphragm region and neck cDNA, RIKEN full-length enriched library, clone:9430020B04 product:weakly similar to PUTATIVE DIPHTHINE SYNTHASE [Schizosaccharomyces pombe], full insert sequence. BC060372 - Mus musculus DPH5 homolog (S. cerevisiae), mRNA (cDNA clone MGC:67875 IMAGE:6391546), complete cds. AK138773 - Mus musculus 0 day neonate thymus cDNA, RIKEN full-length enriched library, clone:A430060L18 product:hypothetical protein, full insert sequence. AK046024 - Mus musculus adult male corpora quadrigemina cDNA, RIKEN full-length enriched library, clone:B230334I05 product:weakly similar to PUTATIVE DIPHTHINE SYNTHASE [Schizosaccharomyces pombe], full insert sequence. AK045548 - Mus musculus adult male corpora quadrigemina cDNA, RIKEN full-length enriched library, clone:B230209N11 product:hypothetical protein, full insert sequence. AK010475 - Mus musculus ES cells cDNA, RIKEN full-length enriched library, clone:2410012M04 product:RIKEN cDNA 2410012M04 gene, full insert sequence.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q9CWQ0 (Reactome details) participates in the following event(s):
R-MMU-5358484 DPH5 transfers four methyl groups from AdoMet to aminocarboxypropyl EEF2 R-MMU-5358493 Synthesis of diphthamide-EEF2 R-MMU-163841 Gamma carboxylation, hypusine formation and arylsulfatase activation R-MMU-597592 Post-translational protein modification R-MMU-392499 Metabolism of proteins