Mouse Gene Dph5 (ENSMUST00000043342.9) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus diphthamide biosynthesis 5 (Dph5), mRNA. (from RefSeq NM_027193)
Gencode Transcript: ENSMUST00000043342.9
Gencode Gene: ENSMUSG00000033554.17
Transcript (Including UTRs)
   Position: mm10 chr3:115,888,183-115,929,332 Size: 41,150 Total Exon Count: 8 Strand: +
Coding Region
   Position: mm10 chr3:115,888,463-115,928,720 Size: 40,258 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr3:115,888,183-115,929,332)mRNA (may differ from genome)Protein (281 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: DPH5_MOUSE
DESCRIPTION: RecName: Full=Diphthine synthase; EC=2.1.1.98; AltName: Full=Diphthamide biosynthesis methyltransferase;
FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF- 2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis.
CATALYTIC ACTIVITY: 3 S-adenosyl-L-methionine + 2-(3-carboxy-3- aminopropyl)-L-histidine = 3 S-adenosyl-L-homocysteine + 2-(3- carboxy-3-(trimethylammonio)propyl)-L-histidine.
PATHWAY: Protein modification; peptidyl-diphthamide biosynthesis.
SIMILARITY: Belongs to the diphthine synthase family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -41.90118-0.355 Picture PostScript Text
3' UTR -168.80612-0.276 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000878 - 4pyrrol_Mease
IPR014777 - 4pyrrole_Mease_sub1
IPR014776 - 4pyrrole_Mease_sub2
IPR004551 - Dphthn_synthase

Pfam Domains:
PF00590 - Tetrapyrrole (Corrin/Porphyrin) Methylases

SCOP Domains:
53790 - Tetrapyrrole methylase

ModBase Predicted Comparative 3D Structure on Q9CWQ0
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologGenome BrowserGenome BrowserNo ortholog
Gene Details    Gene Details
Gene Sorter    Gene Sorter
GenBankRGDEnsemblEnsemblWormBaseSGD
Protein SequenceProtein Sequence Protein SequenceProtein Sequence 
AlignmentAlignment AlignmentAlignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004164 diphthine synthase activity
GO:0008168 methyltransferase activity
GO:0016740 transferase activity

Biological Process:
GO:0008152 metabolic process
GO:0017183 peptidyl-diphthamide biosynthetic process from peptidyl-histidine
GO:0032259 methylation

Cellular Component:
GO:0005575 cellular_component


-  Descriptions from all associated GenBank mRNAs
  AK045127 - Mus musculus 9.5 days embryo parthenogenote cDNA, RIKEN full-length enriched library, clone:B130038I08 product:weakly similar to PUTATIVE DIPHTHINE SYNTHASE [Schizosaccharomyces pombe], full insert sequence.
AK046753 - Mus musculus 4 days neonate thymus cDNA, RIKEN full-length enriched library, clone:B630008G01 product:Weakly similar to putative diphthine synthase, full insert sequence.
AK034652 - Mus musculus 12 days embryo embryonic body between diaphragm region and neck cDNA, RIKEN full-length enriched library, clone:9430020B04 product:weakly similar to PUTATIVE DIPHTHINE SYNTHASE [Schizosaccharomyces pombe], full insert sequence.
BC060372 - Mus musculus DPH5 homolog (S. cerevisiae), mRNA (cDNA clone MGC:67875 IMAGE:6391546), complete cds.
AK138773 - Mus musculus 0 day neonate thymus cDNA, RIKEN full-length enriched library, clone:A430060L18 product:hypothetical protein, full insert sequence.
AK046024 - Mus musculus adult male corpora quadrigemina cDNA, RIKEN full-length enriched library, clone:B230334I05 product:weakly similar to PUTATIVE DIPHTHINE SYNTHASE [Schizosaccharomyces pombe], full insert sequence.
AK045548 - Mus musculus adult male corpora quadrigemina cDNA, RIKEN full-length enriched library, clone:B230209N11 product:hypothetical protein, full insert sequence.
AK010475 - Mus musculus ES cells cDNA, RIKEN full-length enriched library, clone:2410012M04 product:RIKEN cDNA 2410012M04 gene, full insert sequence.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9CWQ0 (Reactome details) participates in the following event(s):

R-MMU-5358484 DPH5 transfers four methyl groups from AdoMet to aminocarboxypropyl EEF2
R-MMU-5358493 Synthesis of diphthamide-EEF2
R-MMU-163841 Gamma carboxylation, hypusine formation and arylsulfatase activation
R-MMU-597592 Post-translational protein modification
R-MMU-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: DPH5_MOUSE, NM_027193, Q6PAC5, Q9CWQ0, uc008rbs.1, uc008rbs.2
UCSC ID: uc008rbs.2
RefSeq Accession: NM_027193
Protein: Q9CWQ0 (aka DPH5_MOUSE)
CCDS: CCDS17782.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.