Mouse Gene Gclm (ENSMUST00000029769.13) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus glutamate-cysteine ligase, modifier subunit (Gclm), mRNA. (from RefSeq NM_008129)
Gencode Transcript: ENSMUST00000029769.13
Gencode Gene: ENSMUSG00000028124.15
Transcript (Including UTRs)
   Position: mm10 chr3:122,245,557-122,270,732 Size: 25,176 Total Exon Count: 7 Strand: +
Coding Region
   Position: mm10 chr3:122,245,986-122,266,397 Size: 20,412 Coding Exon Count: 7 

Page IndexSequence and LinksPrimersCTDRNA StructureProtein Structure
Other SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther NamesModel Information
Methods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr3:122,245,557-122,270,732)mRNA (may differ from genome)Protein (274 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedSOURCEUniProtKBWikipedia

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -198.70429-0.463 Picture PostScript Text
3' UTR -1088.904335-0.251 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR023210 - NADP_OxRdtase_dom

Pfam Domains:
PF00248 - Aldo/keto reductase family

SCOP Domains:
51430 - NAD(P)-linked oxidoreductase

ModBase Predicted Comparative 3D Structure on Q4FJZ6
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
GenBankRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004357 glutamate-cysteine ligase activity
GO:0035226 glutamate-cysteine ligase catalytic subunit binding
GO:0046982 protein heterodimerization activity

Biological Process:
GO:0006534 cysteine metabolic process
GO:0006536 glutamate metabolic process
GO:0006749 glutathione metabolic process
GO:0006750 glutathione biosynthetic process
GO:0006979 response to oxidative stress
GO:0007568 aging
GO:0007584 response to nutrient
GO:0008637 apoptotic mitochondrial changes
GO:0014823 response to activity
GO:0035229 positive regulation of glutamate-cysteine ligase activity
GO:0035729 cellular response to hepatocyte growth factor stimulus
GO:0035733 hepatic stellate cell activation
GO:0042493 response to drug
GO:0043524 negative regulation of neuron apoptotic process
GO:0044344 cellular response to fibroblast growth factor stimulus
GO:0044752 response to human chorionic gonadotropin
GO:0050880 regulation of blood vessel size
GO:0051409 response to nitrosative stress
GO:0051900 regulation of mitochondrial depolarization
GO:0071333 cellular response to glucose stimulus
GO:0071372 cellular response to follicle-stimulating hormone stimulus
GO:0097069 cellular response to thyroxine stimulus
GO:1990830 cellular response to leukemia inhibitory factor
GO:2001237 negative regulation of extrinsic apoptotic signaling pathway

Cellular Component:
GO:0017109 glutamate-cysteine ligase complex


-  Descriptions from all associated GenBank mRNAs
  AK028236 - Mus musculus 10, 11 days embryo whole body cDNA, RIKEN full-length enriched library, clone:2810458D19 product:glutamate-cysteine ligase, modifier subunit, full insert sequence.
AK177930 - Mus musculus cDNA, clone:Y0G0104M17, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000029769, based on BLAT search.
AK172387 - Mus musculus activated spleen cDNA, RIKEN full-length enriched library, clone:F830205K12 product:glutamate-cysteine ligase, modifier subunit, full insert sequence.
BC132404 - Mus musculus glutamate-cysteine ligase, modifier subunit, mRNA (cDNA clone MGC:164035 IMAGE:40130681), complete cds.
BC138783 - Mus musculus glutamate-cysteine ligase, modifier subunit, mRNA (cDNA clone MGC:170410 IMAGE:8861805), complete cds.
U95053 - Mus musculus glutamate-cysteine ligase regulatory subunit (Glclr) mRNA, complete cds.
CT010256 - Mus musculus full open reading frame cDNA clone RZPDo836E0551D for gene Gclm, Glutamate-cysteine ligase, modifier subunit; complete cds, incl. stopcodon.
AK176933 - Mus musculus cDNA, clone:Y0G0101A14, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000029769, based on BLAT search.
AK184377 - Mus musculus cDNA, clone:Y0G0129L24, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000029769, based on BLAT search.
LF289080 - JP 2014500723-A/96583: Polycomb-Associated Non-Coding RNAs.
MA524657 - JP 2018138019-A/96583: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu00480 - Glutathione metabolism
mmu01100 - Metabolic pathways

BioCyc Knowledge Library
GLUTATHIONESYN-PWY - glutathione biosynthesis
PWY-4041 - γ-glutamyl cycle

-  Other Names for This Gene
  Alternate Gene Symbols: NM_008129, Q4FJZ6, Q4FJZ6_MOUSE, uc008reo.1, uc008reo.2, uc008reo.3
UCSC ID: uc008reo.3
RefSeq Accession: NM_008129
Protein: Q4FJZ6 CCDS: CCDS17808.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.