Mouse Gene Ppa2 (ENSMUST00000029644.15) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus pyrophosphatase (inorganic) 2 (Ppa2), transcript variant 1, mRNA. (from RefSeq NM_146141)
Gencode Transcript: ENSMUST00000029644.15
Gencode Gene: ENSMUSG00000028013.16
Transcript (Including UTRs)
   Position: mm10 chr3:133,310,110-133,378,078 Size: 67,969 Total Exon Count: 12 Strand: +
Coding Region
   Position: mm10 chr3:133,310,140-133,377,875 Size: 67,736 Coding Exon Count: 12 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr3:133,310,110-133,378,078)mRNA (may differ from genome)Protein (330 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKB

-  Comments and Description Text from UniProtKB
  ID: IPYR2_MOUSE
DESCRIPTION: RecName: Full=Inorganic pyrophosphatase 2, mitochondrial; EC=3.6.1.1; AltName: Full=Pyrophosphate phospho-hydrolase 2; Short=PPase 2; Flags: Precursor;
CATALYTIC ACTIVITY: Diphosphate + H(2)O = 2 phosphate.
COFACTOR: Magnesium (By similarity).
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Mitochondrion (Potential).
SIMILARITY: Belongs to the PPase family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -9.2030-0.307 Picture PostScript Text
3' UTR -51.70203-0.255 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR008162 - Pyrophosphatase

Pfam Domains:
PF00719 - Inorganic pyrophosphatase

SCOP Domains:
50324 - Inorganic pyrophosphatase

ModBase Predicted Comparative 3D Structure on Q91VM9
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologGenome BrowserNo orthologNo ortholog
Gene Details     
Gene Sorter     
GenBankRGD Ensembl  
Protein SequenceProtein Sequence Protein Sequence  
AlignmentAlignment Alignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000287 magnesium ion binding
GO:0004427 inorganic diphosphatase activity
GO:0004722 protein serine/threonine phosphatase activity
GO:0016787 hydrolase activity
GO:0046872 metal ion binding

Biological Process:
GO:0006470 protein dephosphorylation
GO:0006796 phosphate-containing compound metabolic process
GO:0051881 regulation of mitochondrial membrane potential

Cellular Component:
GO:0005737 cytoplasm
GO:0005739 mitochondrion


-  Descriptions from all associated GenBank mRNAs
  BC011417 - Mus musculus pyrophosphatase (inorganic) 2, mRNA (cDNA clone MGC:6539 IMAGE:2654305), complete cds.
AK090384 - Mus musculus 13 days embryo spinal cord cDNA, RIKEN full-length enriched library, clone:G630072E09 product:similar to INORGANIC PYROPHOSPHATASE 2 [Homo sapiens], full insert sequence.
AK143475 - Mus musculus 8 cells embryo 8 cells cDNA, RIKEN full-length enriched library, clone:E860008H15 product:Similar to pyrophosphatase (Inorganic), full insert sequence.
AK164146 - Mus musculus adult male hippocampus cDNA, RIKEN full-length enriched library, clone:C630007M06 product:unclassifiable, full insert sequence.
AK003660 - Mus musculus 18-day embryo whole body cDNA, RIKEN full-length enriched library, clone:1110013G13 product:SIMILAR TO PYROPHOSPHATASE (INORGANIC) homolog [Mus musculus], full insert sequence.
AK199158 - Mus musculus cDNA, clone:Y1G0130F14, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069016, based on BLAT search.
AK034624 - Mus musculus 12 days embryo embryonic body between diaphragm region and neck cDNA, RIKEN full-length enriched library, clone:9430015H19 product:unclassifiable, full insert sequence.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu00190 - Oxidative phosphorylation

Reactome (by CSHL, EBI, and GO)

Protein Q91VM9 (Reactome details) participates in the following event(s):

R-MMU-449937 pyrophosphate + H2O => 2 orthophosphate [mitochondrial]
R-MMU-71737 Pyrophosphate hydrolysis
R-MMU-379726 Mitochondrial tRNA aminoacylation
R-MMU-1430728 Metabolism
R-MMU-379724 tRNA Aminoacylation
R-MMU-72766 Translation
R-MMU-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: IPYR2_MOUSE, NM_146141, Q3UPK3, Q8BTG5, Q91VM9, Q9D1E3, uc008rkm.1, uc008rkm.2, uc008rkm.3
UCSC ID: uc008rkm.3
RefSeq Accession: NM_146141
Protein: Q91VM9 (aka IPYR2_MOUSE)
CCDS: CCDS17850.1, CCDS80024.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.