Mouse Gene Unc13b (ENSMUST00000163653.7) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus unc-13 homolog B (Unc13b), transcript variant 2, mRNA. (from RefSeq NM_001081413)
Gencode Transcript: ENSMUST00000163653.7
Gencode Gene: ENSMUSG00000028456.18
Transcript (Including UTRs)
   Position: mm10 chr4:43,059,073-43,263,622 Size: 204,550 Total Exon Count: 40 Strand: +
Coding Region
   Position: mm10 chr4:43,059,264-43,263,585 Size: 204,322 Coding Exon Count: 40 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr4:43,059,073-43,263,622)mRNA (may differ from genome)Protein (1601 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: UN13B_MOUSE
DESCRIPTION: RecName: Full=Protein unc-13 homolog B; AltName: Full=Munc13-2; Short=munc13;
FUNCTION: Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity- depending refilling of readily releasable vesicle pool (RRP) (By similarity). Essential for synaptic vesicle maturation in a subset of excitatory/glutamatergic but not inhibitory/GABA-mediated synapses.
SUBUNIT: Interacts with RIMS1 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Golgi apparatus. Cell junction, synapse (By similarity). Note=Localized to synapses (By similarity). Translocates to the Golgi in response to phorbol ester binding.
DOMAIN: The C2 domains are not involved in calcium-dependent phospholipid binding (By similarity).
SIMILARITY: Belongs to the unc-13 family.
SIMILARITY: Contains 3 C2 domains.
SIMILARITY: Contains 1 MHD1 (MUNC13 homology domain 1) domain.
SIMILARITY: Contains 1 MHD2 (MUNC13 homology domain 2) domain.
SIMILARITY: Contains 1 phorbol-ester/DAG-type zinc finger.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -94.30191-0.494 Picture PostScript Text
3' UTR -1.2037-0.032 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000008 - C2_Ca-dep
IPR008973 - C2_Ca/lipid-bd_dom_CaLB
IPR020477 - C2_dom
IPR018029 - C2_membr_targeting
IPR010439 - Ca-dep_secretion_activator
IPR014770 - Munc13_1
IPR014772 - Munc13_dom-2
IPR019558 - Munc13_subgr_dom-2
IPR002219 - Prot_Kinase_C-like_PE/DAG-bd

Pfam Domains:
PF00130 - Phorbol esters/diacylglycerol binding domain (C1 domain)
PF00168 - C2 domain
PF06292 - Domain of Unknown Function (DUF1041)
PF10540 - Munc13 (mammalian uncoordinated) homology domain

SCOP Domains:
49562 - C2 domain (Calcium/lipid-binding domain, CaLB)
57889 - Cysteine-rich domain

ModBase Predicted Comparative 3D Structure on Q9Z1N9
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
GenBankRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001566 non-kinase phorbol ester receptor activity
GO:0005509 calcium ion binding
GO:0005516 calmodulin binding
GO:0005543 phospholipid binding
GO:0017075 syntaxin-1 binding
GO:0019905 syntaxin binding
GO:0019992 diacylglycerol binding
GO:0030742 GTP-dependent protein binding
GO:0046872 metal ion binding

Biological Process:
GO:0006887 exocytosis
GO:0007268 chemical synaptic transmission
GO:0007528 neuromuscular junction development
GO:0010808 positive regulation of synaptic vesicle priming
GO:0016081 synaptic vesicle docking
GO:0016082 synaptic vesicle priming
GO:0031914 negative regulation of synaptic plasticity
GO:0035249 synaptic transmission, glutamatergic
GO:0035556 intracellular signal transduction
GO:0043065 positive regulation of apoptotic process
GO:0045921 positive regulation of exocytosis
GO:0048172 regulation of short-term neuronal synaptic plasticity
GO:0050714 positive regulation of protein secretion
GO:0060384 innervation
GO:0061669 spontaneous neurotransmitter secretion
GO:0061789 dense core granule priming
GO:0071333 cellular response to glucose stimulus
GO:0090382 phagosome maturation
GO:0097151 positive regulation of inhibitory postsynaptic potential
GO:0099525 presynaptic dense core vesicle exocytosis
GO:1900426 positive regulation of defense response to bacterium
GO:0061790 dense core granule docking

Cellular Component:
GO:0005737 cytoplasm
GO:0005794 Golgi apparatus
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0030054 cell junction
GO:0031594 neuromuscular junction
GO:0032009 early phagosome
GO:0043195 terminal bouton
GO:0044305 calyx of Held
GO:0045202 synapse
GO:0048786 presynaptic active zone
GO:0097060 synaptic membrane
GO:0097470 ribbon synapse
GO:0098793 presynapse


-  Descriptions from all associated GenBank mRNAs
  AF115848 - Mus musculus renal munc13 mRNA, complete cds.
BC024771 - Mus musculus unc-13 homolog B (C. elegans), mRNA (cDNA clone IMAGE:5367645).
AK161056 - Mus musculus 0 day neonate skin cDNA, RIKEN full-length enriched library, clone:4632410H01 product:Renal munc13 homolog [Mus musculus], full insert sequence.
BC157967 - Mus musculus unc-13 homolog B (C. elegans), mRNA (cDNA clone MGC:189948 IMAGE:9088135), complete cds.
BC158025 - Mus musculus unc-13 homolog B (C. elegans), mRNA (cDNA clone MGC:189830 IMAGE:9007330), complete cds.
BC166023 - Synthetic construct Mus musculus clone IMAGE:100066323, MGC:195460 unc-13 homolog B (C. elegans) (Unc13b) mRNA, encodes complete protein.
AK122422 - Mus musculus premature mRNA for mKIAA4254 protein.
AK039015 - Mus musculus adult male hypothalamus cDNA, RIKEN full-length enriched library, clone:A230085L07 product:unc13 homolog (C. elegans) 1, full insert sequence.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9Z1N9 (Reactome details) participates in the following event(s):

R-MMU-210426 Glutamate synaptic vesicle docking and priming
R-MMU-372505 Acetylcholine synaptic vesicle docking and priming
R-MMU-374922 Noradrenalin synaptic vesicle docking and priming
R-MMU-380574 Dopamine synaptic vesicle docking and priming
R-MMU-380905 Serotonin loaded synaptic vesicle docking and priming
R-MMU-210430 release of L-Glutamate at the synapse
R-MMU-372529 Release of acetylcholine at the synapse
R-MMU-374899 Release of noradrenaline at the synapse
R-MMU-380869 Release of docked dopamine loaded synaptic vesicle
R-MMU-380901 Release of docked serotonin loaded synaptic vesicle
R-MMU-210500 Glutamate Neurotransmitter Release Cycle
R-MMU-264642 Acetylcholine Neurotransmitter Release Cycle
R-MMU-181430 Norepinephrine Neurotransmitter Release Cycle
R-MMU-212676 Dopamine Neurotransmitter Release Cycle
R-MMU-181429 Serotonin Neurotransmitter Release Cycle
R-MMU-112310 Neurotransmitter release cycle
R-MMU-112315 Transmission across Chemical Synapses
R-MMU-112316 Neuronal System

-  Other Names for This Gene
  Alternate Gene Symbols: A2AG43, B2RXT0, B2RXY6, NM_001081413, Q6BCX2, Q9Z1N9, uc008spg.1, uc008spg.2, uc008spg.3, uc008spg.4, UN13B_MOUSE, Unc13a
UCSC ID: uc008spg.4
RefSeq Accession: NM_001081413
Protein: Q9Z1N9 (aka UN13B_MOUSE or U13B_MOUSE)
CCDS: CCDS38738.1, CCDS51161.1, CCDS80089.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.