Mouse Gene Hgf (ENSMUST00000199581.4) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus hepatocyte growth factor (Hgf), transcript variant 3, mRNA. (from RefSeq NM_010427)
RefSeq Summary (NM_010427): This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the hepatocyte growth factor alpha and beta chains, which heterodimerize to form the mature active protein. Although this protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Homozygous knockout mice for this gene exhibit embryonic lethality due to impaired development of the placenta and liver. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015].
Gencode Transcript: ENSMUST00000199581.4
Gencode Gene: ENSMUSG00000028864.7
Transcript (Including UTRs)
   Position: mm10 chr5:16,553,550-16,620,152 Size: 66,603 Total Exon Count: 19 Strand: +
Coding Region
   Position: mm10 chr5:16,553,928-16,618,993 Size: 65,066 Coding Exon Count: 18 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr5:16,553,550-16,620,152)mRNA (may differ from genome)Protein (728 aa)
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BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: HGF_MOUSE
DESCRIPTION: RecName: Full=Hepatocyte growth factor; AltName: Full=Hepatopoietin-A; AltName: Full=Scatter factor; Short=SF; Contains: RecName: Full=Hepatocyte growth factor alpha chain; Contains: RecName: Full=Hepatocyte growth factor beta chain; Flags: Precursor;
FUNCTION: HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. It has no detectable protease activity. Activating ligand for the receptor tyrosine kinase MET by binding and promoting its dimerization.
SUBUNIT: Dimer of an alpha chain and a beta chain linked by a disulfide bond.
SIMILARITY: Belongs to the peptidase S1 family. Plasminogen subfamily.
SIMILARITY: Contains 4 kringle domains.
SIMILARITY: Contains 1 PAN domain.
SIMILARITY: Contains 1 peptidase S1 domain.
SEQUENCE CAUTION: Sequence=CAA51054.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

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-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -39.50122-0.324 Picture PostScript Text
3' UTR -229.501159-0.198 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR024174 - HGF_MST1
IPR000001 - Kringle
IPR013806 - Kringle-like
IPR018056 - Kringle_CS
IPR003014 - PAN-1_domain
IPR003609 - Pan_app
IPR009003 - Pept_cys/ser_Trypsin-like
IPR001254 - Peptidase_S1_S6
IPR001314 - Peptidase_S1A

Pfam Domains:
PF00024 - PAN domain
PF00051 - Kringle domain
PF00089 - Trypsin

SCOP Domains:
50494 - Trypsin-like serine proteases
57414 - Hairpin loop containing domain-like
57440 - Kringle-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2QJ4 - X-ray 3HMR - X-ray


ModBase Predicted Comparative 3D Structure on Q08048
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
GenBankRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004252 serine-type endopeptidase activity
GO:0005515 protein binding
GO:0008083 growth factor activity
GO:0042056 chemoattractant activity
GO:0042802 identical protein binding
GO:0046982 protein heterodimerization activity

Biological Process:
GO:0000187 activation of MAPK activity
GO:0000902 cell morphogenesis
GO:0001889 liver development
GO:0006508 proteolysis
GO:0008283 cell proliferation
GO:0008284 positive regulation of cell proliferation
GO:0010469 regulation of receptor activity
GO:0014068 positive regulation of phosphatidylinositol 3-kinase signaling
GO:0030212 hyaluronan metabolic process
GO:0030335 positive regulation of cell migration
GO:0031100 animal organ regeneration
GO:0031643 positive regulation of myelination
GO:0032715 negative regulation of interleukin-6 production
GO:0032733 positive regulation of interleukin-10 production
GO:0033137 negative regulation of peptidyl-serine phosphorylation
GO:0035729 cellular response to hepatocyte growth factor stimulus
GO:0043066 negative regulation of apoptotic process
GO:0043154 negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0045766 positive regulation of angiogenesis
GO:0048012 hepatocyte growth factor receptor signaling pathway
GO:0050728 negative regulation of inflammatory response
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050918 positive chemotaxis
GO:0051450 myoblast proliferation
GO:0060326 cell chemotaxis
GO:0060665 regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling
GO:0070572 positive regulation of neuron projection regeneration
GO:0090201 negative regulation of release of cytochrome c from mitochondria
GO:1900744 regulation of p38MAPK cascade
GO:1901299 negative regulation of hydrogen peroxide-mediated programmed cell death
GO:1902042 negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
GO:1902947 regulation of tau-protein kinase activity
GO:2000573 positive regulation of DNA biosynthetic process

Cellular Component:
GO:0005576 extracellular region
GO:0005615 extracellular space


-  Descriptions from all associated GenBank mRNAs
  AK052289 - Mus musculus 13 days embryo heart cDNA, RIKEN full-length enriched library, clone:D330021I23 product:hepatocyte growth factor, full insert sequence.
AK042121 - Mus musculus 3 days neonate thymus cDNA, RIKEN full-length enriched library, clone:A630060C16 product:hepatocyte growth factor, full insert sequence.
AK142159 - Mus musculus 13 days embryo heart cDNA, RIKEN full-length enriched library, clone:D330003C05 product:hepatocyte growth factor, full insert sequence.
BC119228 - Mus musculus hepatocyte growth factor, mRNA (cDNA clone MGC:155544 IMAGE:8733977), complete cds.
BC144764 - Mus musculus cDNA clone IMAGE:9053312, containing frame-shift errors.
AK082461 - Mus musculus 0 day neonate cerebellum cDNA, RIKEN full-length enriched library, clone:C230052L06 product:hepatocyte growth factor, full insert sequence.
X72307 - M.musculus mRNA for hepatocyte growth factor.
X84046 - M.musculus mRNA for hepatocyte growth factor/scatter factor.
AF042856 - Mus musculus hepatocyte growth factor NK1 mRNA, complete cds.
D10212 - Mus musculus mRNA for hepatocyte growth factor, complete cds.
D10213 - Mus musculus mRNA for hepatocyte growth factor, complete cds.
S71816 - hepatocyte growth factor [mice, liver, mRNA Partial, 2281 nt].

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu04060 - Cytokine-cytokine receptor interaction
mmu04510 - Focal adhesion
mmu05144 - Malaria
mmu05200 - Pathways in cancer
mmu05211 - Renal cell carcinoma
mmu05218 - Melanoma

BioCarta from NCI Cancer Genome Anatomy Project
m_metPathway - Signaling of Hepatocyte Growth Factor Receptor

Reactome (by CSHL, EBI, and GO)

Protein Q08048 (Reactome details) participates in the following event(s):

R-NUL-1266699 PPBSF is a complex of Il7 and Hgf(495-728)
R-MMU-6800200 HPN heterodimer cleaves pro-HGF to form HGF dimer
R-MMU-6800299 HGFAC cleaves pro-HGF to form HGF dimer
R-MMU-6800298 HGF dimer binds MET
R-MMU-481007 Exocytosis of platelet alpha granule contents
R-MMU-6806957 MET receptor dimerizes
R-MMU-6807008 PTPRJ dephosphorylates MET
R-MMU-6807027 PTPN1 and PTPN2 dephosphorylate MET
R-MMU-8851804 Activated MET recruits GRB2-1:SOS1
R-MMU-8851842 MUC20 binds MET and inhibits GRB2 binding
R-MMU-8851859 Activated MET binds RANBP9 and SOS1
R-MMU-8851866 MET binds RANBP10
R-MMU-8851888 MET binds SHC1-2
R-MMU-8851908 Activated MET binds GAB1
R-MMU-8851919 Activated MET binds GAB1 and GRB2
R-MMU-8874079 PTK2 binds activated MET
R-MMU-8875523 MET binds TNS3
R-MMU-8875531 MET binds TNS4
R-MMU-8875816 STAT3 binds activated MET receptor
R-MMU-6806974 MET dimers autophosphorylate
R-MMU-8875817 MET phosphorylates STAT3
R-MMU-8851900 SHC1-2 bound to MET recruits GRB2:SOS1
R-MMU-8851890 MET phosphorylates SHC1-2
R-MMU-8874078 PTK2 autophosphorylates
R-MMU-8874083 p-Y397-PTK2 binds SRC
R-MMU-8874685 CBL binds MET
R-MMU-8851954 Phosphorylated GAB1 recruits PI3K to MET
R-MMU-8865994 GAB1 binds PTPN11
R-MMU-8875540 GAB1 recruits CRK,CRKL to MET
R-MMU-8851933 MET phosphorylates GAB1
R-MMU-8851877 RAS guanyl nucleotide exchange by SOS1 associated with RANBP9 and MET
R-MMU-8851827 RAS guanyl nucleotide exchange by MET-bound GRB2:SOS1
R-MMU-8874082 MET phosphorylates PTK2
R-MMU-8874080 SRC phosphorylates PTK2
R-MMU-8875451 MET phosphorylates CBL
R-MMU-8875183 CBL monoubiquitinates activated MET
R-MMU-8875482 CBL recruits CIN85:endophilin to ubiquitinated MET
R-MMU-8875558 RAPGEF1 binds CRK,CRKL
R-MMU-8851899 RAS guanyl nucleotide exchange by SOS1 bound to GRB2, SCH1-2 and MET
R-MMU-5672965 RAS GEFs promote RAS nucleotide exchange
R-MMU-8852019 MET bound PI3K generates PIP3
R-MMU-8875490 EPS15 and HGS bind ubiquitinated MET
R-MMU-2316434 PI3K phosphorylates PIP2 to PIP3
R-MMU-8875568 RAPGEF1 activates RAP1
R-MMU-6806942 MET Receptor Activation
R-MMU-6806834 Signaling by MET
R-MMU-114608 Platelet degranulation
R-MMU-9006934 Signaling by Receptor Tyrosine Kinases
R-MMU-76005 Response to elevated platelet cytosolic Ca2+
R-MMU-6807004 Negative regulation of MET activity
R-MMU-8851805 MET activates RAS signaling
R-MMU-8851907 MET activates PI3K/AKT signaling
R-MMU-8874081 MET activates PTK2 signaling
R-MMU-8875513 MET interacts with TNS proteins
R-MMU-8875791 MET activates STAT3
R-MMU-8875656 MET receptor recycling
R-MMU-162582 Signal Transduction
R-MMU-76002 Platelet activation, signaling and aggregation
R-MMU-8875878 MET promotes cell motility
R-MMU-109582 Hemostasis
R-MMU-8865999 MET activates PTPN11
R-MMU-8875555 MET activates RAP1 and RAC1
R-MMU-5673001 RAF/MAP kinase cascade
R-MMU-5684996 MAPK1/MAPK3 signaling
R-MMU-1257604 PIP3 activates AKT signaling
R-MMU-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
R-MMU-9032759 NTRK2 activates RAC1
R-MMU-5683057 MAPK family signaling cascades
R-MMU-9006925 Intracellular signaling by second messengers
R-MMU-199418 Negative regulation of the PI3K/AKT network
R-MMU-9032500 Activated NTRK2 signals through FYN
R-MMU-9006115 Signaling by NTRK2 (TRKB)
R-MMU-166520 Signaling by NTRKs

-  Other Names for This Gene
  Alternate Gene Symbols: HGF_MOUSE, NM_010427, O55027, Q08048, Q53WS5, Q61662, Q64007, Q6LBE6, uc008wnf.1, uc008wnf.2, uc008wnf.3
UCSC ID: uc008wnf.3
RefSeq Accession: NM_010427
Protein: Q08048 (aka HGF_MOUSE)
CCDS: CCDS19097.1, CCDS80213.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.