Mouse Gene Eif3b (ENSMUST00000100507.7) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus eukaryotic translation initiation factor 3, subunit B (Eif3b), mRNA. (from RefSeq NM_133916)
Gencode Transcript: ENSMUST00000100507.7
Gencode Gene: ENSMUSG00000056076.13
Transcript (Including UTRs)
   Position: mm10 chr5:140,419,328-140,443,360 Size: 24,033 Total Exon Count: 19 Strand: +
Coding Region
   Position: mm10 chr5:140,419,361-140,442,423 Size: 23,063 Coding Exon Count: 18 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr5:140,419,328-140,443,360)mRNA (may differ from genome)Protein (803 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
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-  Comments and Description Text from UniProtKB
  ID: EIF3B_MOUSE
DESCRIPTION: RecName: Full=Eukaryotic translation initiation factor 3 subunit B; Short=eIF3b; AltName: Full=Eukaryotic translation initiation factor 3 subunit 9; AltName: Full=eIF-3-eta; AltName: Full=eIF3 p116;
FUNCTION: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
SUBUNIT: Interacts with UPF2 (By similarity). Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex may also interact with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation may lead to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
TISSUE SPECIFICITY: Ubiquitously expressed.
DOMAIN: The RRM domain mediates interaction with EIF3J (By similarity).
PTM: Phosphorylated. Phosphorylation is enhanced upon serum stimulation (By similarity).
DISRUPTION PHENOTYPE: Embryonic death.
SIMILARITY: Belongs to the eIF-3 subunit B family.
SIMILARITY: Contains 1 RRM (RNA recognition motif) domain.
SIMILARITY: Contains 5 WD repeats.

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -10.0033-0.303 Picture PostScript Text
3' UTR -191.10500-0.382 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011042 - 6-blade_b-propeller_TolB-like
IPR011400 - eIF3b
IPR012677 - Nucleotide-bd_a/b_plait
IPR000504 - RRM_dom
IPR013979 - TIF2A_beta_prop-like
IPR015943 - WD40/YVTN_repeat-like_dom

Pfam Domains:
PF00076 - RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
PF08662 - Eukaryotic translation initiation factor eIF2A

SCOP Domains:
47661 - t-snare proteins
48726 - Immunoglobulin
50952 - Soluble quinoprotein glucose dehydrogenase
50960 - TolB, C-terminal domain
63825 - YWTD domain
63829 - Calcium-dependent phosphotriesterase
69304 - Tricorn protease N-terminal domain
101898 - NHL repeat
50965 - Galactose oxidase, central domain
75011 - 3-carboxy-cis,cis-mucoante lactonizing enzyme
101908 - Putative isomerase YbhE
117289 - Nucleoporin domain
50969 - YVTN repeat-like/Quinoprotein amine dehydrogenase
50974 - Nitrous oxide reductase, N-terminal domain
50978 - WD40 repeat-like
50993 - Peptidase/esterase 'gauge' domain
69322 - Tricorn protease domain 2
50998 - Quinoprotein alcohol dehydrogenase-like
51004 - C-terminal (heme d1) domain of cytochrome cd1-nitrite reductase
82171 - DPP6 N-terminal domain-like
54928 - RNA-binding domain, RBD
56672 - DNA/RNA polymerases

ModBase Predicted Comparative 3D Structure on Q8JZQ9
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologGenome BrowserNo orthologNo ortholog
Gene Details    Gene Details
Gene Sorter    Gene Sorter
GenBankRGDEnsemblEnsemblWormBaseSGD
Protein SequenceProtein Sequence Protein Sequence  
AlignmentAlignment Alignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003676 nucleic acid binding
GO:0003723 RNA binding
GO:0003743 translation initiation factor activity
GO:0005515 protein binding
GO:0031369 translation initiation factor binding

Biological Process:
GO:0006412 translation
GO:0006413 translational initiation
GO:0006446 regulation of translational initiation
GO:0075522 IRES-dependent viral translational initiation
GO:0075525 viral translational termination-reinitiation

Cellular Component:
GO:0005737 cytoplasm
GO:0005852 eukaryotic translation initiation factor 3 complex
GO:0071541 eukaryotic translation initiation factor 3 complex, eIF3m


-  Descriptions from all associated GenBank mRNAs
  AK170938 - Mus musculus NOD-derived CD11c +ve dendritic cells cDNA, RIKEN full-length enriched library, clone:F630211E12 product:eukaryotic translation initiation factor 3, subunit 9 (eta), full insert sequence.
AK033722 - Mus musculus adult male cecum cDNA, RIKEN full-length enriched library, clone:9130232K10 product:EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT 9 (EIF-3 ETA) (EIF3 P116) (EIF3 P110) homolog [Homo sapiens], full insert sequence.
AK167618 - Mus musculus 12 days pregnant adult female placenta cDNA, RIKEN full-length enriched library, clone:I530025F11 product:eukaryotic translation initiation factor 3, subunit 9 (eta), full insert sequence.
AK089978 - Mus musculus RCB-0559 K-1 . F1 cDNA, RIKEN full-length enriched library, clone:G430041C21 product:EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT 9 (EIF-3 ETA) (EIF3 P116) (EIF3 P110) homolog [Homo sapiens], full insert sequence.
BC031704 - Mus musculus eukaryotic translation initiation factor 3, subunit B, mRNA (cDNA clone MGC:25599 IMAGE:4023277), complete cds.
BC023767 - Mus musculus eukaryotic translation initiation factor 3, subunit B, mRNA (cDNA clone MGC:38325 IMAGE:5343631), complete cds.
BC051065 - Mus musculus eukaryotic translation initiation factor 3, subunit B, mRNA (cDNA clone MGC:59168 IMAGE:5343631), complete cds.
AK195154 - Mus musculus cDNA, clone:Y1G0117I22, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK214205 - Mus musculus cDNA, clone:Y2G0131A15, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
BC007175 - Mus musculus eukaryotic translation initiation factor 3, subunit B, mRNA (cDNA clone IMAGE:3493441), partial cds.
AK204322 - Mus musculus cDNA, clone:Y1G0147P19, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK198242 - Mus musculus cDNA, clone:Y1G0127I21, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK206288 - Mus musculus cDNA, clone:Y2G0104F18, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK194329 - Mus musculus cDNA, clone:Y1G0115A02, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK203889 - Mus musculus cDNA, clone:Y1G0146I21, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK202933 - Mus musculus cDNA, clone:Y1G0143D08, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK204353 - Mus musculus cDNA, clone:Y1G0148C01, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK190217 - Mus musculus cDNA, clone:Y1G0102B13, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK190362 - Mus musculus cDNA, clone:Y1G0102I08, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK193854 - Mus musculus cDNA, clone:Y1G0113J01, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK193918 - Mus musculus cDNA, clone:Y1G0113M02, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK196728 - Mus musculus cDNA, clone:Y1G0122L19, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK198886 - Mus musculus cDNA, clone:Y1G0129H13, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK199123 - Mus musculus cDNA, clone:Y1G0130D21, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK204714 - Mus musculus cDNA, clone:Y1G0149G09, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK211352 - Mus musculus cDNA, clone:Y2G0121H15, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK219469 - Mus musculus cDNA, clone:Y2G0148J02, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK189975 - Mus musculus cDNA, clone:Y1G0101F21, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK202262 - Mus musculus cDNA, clone:Y1G0140O06, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK218594 - Mus musculus cDNA, clone:Y2G0145K22, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK219456 - Mus musculus cDNA, clone:Y2G0148I09, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK218044 - Mus musculus cDNA, clone:Y2G0143N05, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK205605 - Mus musculus cDNA, clone:Y2G0102D21, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000069974, based on BLAT search.
AK202344 - Mus musculus cDNA, clone:Y1G0141D03, strand:unspecified.
AK014117 - Mus musculus 13 days embryo head cDNA, RIKEN full-length enriched library, clone:3110032P20 product:weakly similar to EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT 9 (EIF-3 ETA) (EIF3 P116) (EIF3 P110) [Homo sapiens], full insert sequence.
AK186302 - Mus musculus cDNA, clone:Y0G0136O07, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000031544, based on BLAT search.
AK211163 - Mus musculus cDNA, clone:Y2G0120N15, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000031544, based on BLAT search.
AK203959 - Mus musculus cDNA, clone:Y1G0146N07, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000031544, based on BLAT search.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu03013 - RNA transport

Reactome (by CSHL, EBI, and GO)

Protein Q8JZQ9 (Reactome details) participates in the following event(s):

R-MMU-72676 eIF3 and eIF1A bind to the 40S subunit
R-MMU-157849 Formation of translation initiation complexes containing mRNA that does not circularize
R-MMU-72691 Formation of the 43S pre-initiation complex
R-MMU-72621 Ribosomal scanning
R-MMU-156823 Association of phospho-L13a with GAIT element of Ceruloplasmin mRNA
R-MMU-72689 Formation of a pool of free 40S subunits
R-MMU-72649 Translation initiation complex formation
R-MMU-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-MMU-72737 Cap-dependent Translation Initiation
R-MMU-72702 Ribosomal scanning and start codon recognition
R-MMU-156827 L13a-mediated translation
R-MMU-72662 Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S
R-MMU-72613 Eukaryotic Translation Initiation
R-MMU-72766 Translation
R-MMU-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: EIF3B_MOUSE, Eif3s9, NM_133916, Q8JZQ9, Q922K2, uc009ahr.1, uc009ahr.2
UCSC ID: uc009ahr.2
RefSeq Accession: NM_133916
Protein: Q8JZQ9 (aka EIF3B_MOUSE)
CCDS: CCDS19819.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.