Mouse Gene Radil (ENSMUST00000110784.7) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Downstream effector of Rap required for cell adhesion and migration of neural crest precursors during development. (from UniProt Q69Z89)
Gencode Transcript: ENSMUST00000110784.7
Gencode Gene: ENSMUSG00000029576.17
Transcript (Including UTRs)
   Position: mm10 chr5:142,484,867-142,509,659 Size: 24,793 Total Exon Count: 14 Strand: -
Coding Region
   Position: mm10 chr5:142,485,143-142,508,132 Size: 22,990 Coding Exon Count: 13 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther NamesModel Information
Methods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr5:142,484,867-142,509,659)mRNA (may differ from genome)Protein (830 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblExonPrimerGeneCardsMGIPubMed
SOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: RADIL_MOUSE
DESCRIPTION: RecName: Full=Ras-associating and dilute domain-containing protein;
FUNCTION: Downstream effector of Rap required for cell adhesion and migration of neural crest precursors during development.
SUBUNIT: Interacts with RAP1A; in a GTP-dependent manner. Does not interact with members of the Ras family (By similarity).
SIMILARITY: Belongs to the RADIL family.
SIMILARITY: Contains 1 dilute domain.
SIMILARITY: Contains 1 FHA domain.
SIMILARITY: Contains 1 PDZ (DHR) domain.
SIMILARITY: Contains 1 Ras-associating domain.
SEQUENCE CAUTION: Sequence=BAC38904.1; Type=Frameshift; Positions=985; Sequence=BAD32555.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -151.40337-0.449 Picture PostScript Text
3' UTR -68.50276-0.248 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR018444 - Dil_domain
IPR002710 - Dilute
IPR000253 - FHA_dom
IPR001478 - PDZ
IPR000159 - Ras-assoc
IPR008984 - SMAD_FHA_domain

Pfam Domains:
PF00595 - PDZ domain (Also known as DHR or GLGF)
PF01843 - DIL domain

SCOP Domains:
49879 - SMAD/FHA domain
50156 - PDZ domain-like

ModBase Predicted Comparative 3D Structure on Q69Z89
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
GenBankRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding

Biological Process:
GO:0007155 cell adhesion
GO:0007165 signal transduction
GO:0007275 multicellular organism development
GO:0034446 substrate adhesion-dependent cell spreading

Cellular Component:
GO:0005874 microtubule


-  Descriptions from all associated GenBank mRNAs
  BC056483 - Mus musculus Ras association and DIL domains, mRNA (cDNA clone MGC:67453 IMAGE:5696312), complete cds.
AK083410 - Mus musculus 9 days embryo whole body cDNA, RIKEN full-length enriched library, clone:D030002M07 product:hypothetical RA domain containing protein, full insert sequence.
AK052964 - Mus musculus 15 days embryo head cDNA, RIKEN full-length enriched library, clone:D930005D10 product:hypothetical Gram-positive cocci surface protein 'anchoring' hexapeptide/RA domain/PDZ domain (also known as DHR or GLGF) containing protein, full insert sequence.
AK154169 - Mus musculus NOD-derived CD11c +ve dendritic cells cDNA, RIKEN full-length enriched library, clone:F630006B20 product:Hypothetical Gram-positive cocci surface protein 'anchoring' hexapeptide/RA domain/PDZ domain, full insert sequence.
AK173277 - Mus musculus mRNA for mKIAA1849 protein.
AK079539 - Mus musculus adult male hypothalamus cDNA, RIKEN full-length enriched library, clone:A230045B21 product:hypothetical Gram-positive cocci surface protein 'anchoring' hexapeptide/PDZ domain (also known as DHR or GLGF) containing protein, full insert sequence.
AK162917 - Mus musculus adult male spinal cord cDNA, RIKEN full-length enriched library, clone:A330034L03 product:Hypothetical Gram-positive cocci surface protein 'anchoring' hexapeptide/RA domain/PDZ domain homolog [Mus musculus], full insert sequence.
AK038570 - Mus musculus adult male hypothalamus cDNA, RIKEN full-length enriched library, clone:A230044L11 product:hypothetical RA domain containing protein, full insert sequence.
AK163852 - Mus musculus 16 days embryo head cDNA, RIKEN full-length enriched library, clone:C130086G02 product:Hypothetical Gram-positive cocci surface protein 'anchoring' hexapeptide/RA domain/PDZ domain homolog [Mus musculus], full insert sequence.

-  Other Names for This Gene
  Alternate Gene Symbols: AK079539, Kiaa1849, Q69Z89, Q8BNL0, Q8C549, Q8C6X2, Q8CAL1, RADIL_MOUSE, uc009ais.1, uc009ais.2, uc009ais.3
UCSC ID: uc009ais.3
RefSeq Accession: NM_178702
Protein: Q69Z89 (aka RADIL_MOUSE)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.