Mouse Gene Try5 (ENSMUST00000064324.11) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus trypsin 5 (Try5), mRNA. (from RefSeq NM_001003405)
Gencode Transcript: ENSMUST00000064324.11
Gencode Gene: ENSMUSG00000036938.17
Transcript (Including UTRs)
   Position: mm10 chr6:41,311,233-41,314,711 Size: 3,479 Total Exon Count: 5 Strand: -
Coding Region
   Position: mm10 chr6:41,311,277-41,314,695 Size: 3,419 Coding Exon Count: 5 

Page IndexSequence and LinksUniProtKB CommentsPrimersRNA StructureProtein Structure
Other SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther NamesModel Information
Methods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr6:41,311,233-41,314,711)mRNA (may differ from genome)Protein (246 aa)
Gene SorterGenome BrowserOther Species FASTATable SchemaAlphaFoldBioGPS
EnsemblEntrez GeneExonPrimerGeneCardsMGIPubMed
ReactomeSOURCEUniProtKB

-  Comments and Description Text from UniProtKB
  ID: Q9QUK9_MOUSE
DESCRIPTION: SubName: Full=MCG15083; SubName: Full=Protein Try5; SubName: Full=RIKEN cDNA 1810049H19 gene; SubName: Full=TESP4; SubName: Full=Trypsinogen 9;
SIMILARITY: Belongs to the peptidase S1 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR 0.00160.000 Picture PostScript Text
3' UTR -1.4044-0.032 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR009003 - Pept_cys/ser_Trypsin-like
IPR018114 - Peptidase_S1/S6_AS
IPR001254 - Peptidase_S1_S6
IPR001314 - Peptidase_S1A

Pfam Domains:
PF00089 - Trypsin

SCOP Domains:
50494 - Trypsin-like serine proteases

ModBase Predicted Comparative 3D Structure on Q9QUK9
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
      
      
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004252 serine-type endopeptidase activity
GO:0005509 calcium ion binding
GO:0008233 peptidase activity
GO:0008236 serine-type peptidase activity
GO:0016787 hydrolase activity

Biological Process:
GO:0006508 proteolysis
GO:0007586 digestion

Cellular Component:
GO:0005615 extracellular space


-  Descriptions from all associated GenBank mRNAs
  AK131898 - Mus musculus adult male stomach cDNA, RIKEN full-length enriched library, clone:2210415M03 product:TESP4 (0910001B19RIK protein) (Trypsinogen 9), full insert sequence.
AK160484 - Mus musculus adult male stomach cDNA, RIKEN full-length enriched library, clone:2210402E17 product:TESP4 (0910001B19RIK protein) (Trypsinogen 9), full insert sequence.
AK007843 - Mus musculus 10 day old male pancreas cDNA, RIKEN full-length enriched library, clone:1810049H19 product:TESP4 (0910001B19RIK protein) (Trypsinogen 9), full insert sequence.
BC139220 - Mus musculus RIKEN cDNA 1810049H19 gene, mRNA (cDNA clone MGC:170847 IMAGE:8862242), complete cds.
AB009661 - Mus musculus mRNA for TESP4, complete cds.
BC139223 - Mus musculus RIKEN cDNA 1810049H19 gene, mRNA (cDNA clone MGC:170850 IMAGE:8862245), complete cds.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9QUK9 (Reactome details) participates in the following event(s):

R-MMU-5693319 CTRC hydrolyses PRSS1
R-MMU-6798751 Exocytosis of azurophil granule lumen proteins
R-MMU-6798745 Exocytosis of tertiary granule lumen proteins
R-MMU-1454822 Collagen type I degradation by MMP1,2,8,13, PRSS2
R-MMU-1474197 Collagen type II degradation by MMP1,3,8,13,PRSS2
R-MMU-1592316 Initial activation of proMMP1
R-MMU-1592371 Initial activation of proMMP3
R-MMU-1602488 Initial activation of proMMP13 by plasmin and trypsin
R-MMU-1604712 Initial activation of proMMP7 by trypsin
R-MMU-1604722 Activation of proMMP9 by proteases
R-MMU-196741 Cobalamin (Cbl, vitamin B12) transport and metabolism
R-MMU-6798695 Neutrophil degranulation
R-MMU-196849 Metabolism of water-soluble vitamins and cofactors
R-MMU-1442490 Collagen degradation
R-MMU-1592389 Activation of Matrix Metalloproteinases
R-MMU-168249 Innate Immune System
R-MMU-6803157 Antimicrobial peptides
R-MMU-196854 Metabolism of vitamins and cofactors
R-MMU-1474228 Degradation of the extracellular matrix
R-MMU-168256 Immune System
R-MMU-1430728 Metabolism
R-MMU-1474244 Extracellular matrix organization

-  Other Names for This Gene
  Alternate Gene Symbols: mCG_15083, NM_001003405, Q9QUK9, Q9QUK9_MOUSE, trypsinogen, uc009bot.1, uc009bot.2, uc009bot.3
UCSC ID: uc009bot.3
RefSeq Accession: NM_001003405
Protein: Q9QUK9 CCDS: CCDS20045.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.