Mouse Gene Dok1 (ENSMUST00000089651.5) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus docking protein 1 (Dok1), transcript variant 1, mRNA. (from RefSeq NM_010070)
Gencode Transcript: ENSMUST00000089651.5
Gencode Gene: ENSMUSG00000068335.6
Transcript (Including UTRs)
   Position: mm10 chr6:83,030,934-83,033,471 Size: 2,538 Total Exon Count: 5 Strand: -
Coding Region
   Position: mm10 chr6:83,031,251-83,033,430 Size: 2,180 Coding Exon Count: 5 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr6:83,030,934-83,033,471)mRNA (may differ from genome)Protein (482 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: DOK1_MOUSE
DESCRIPTION: RecName: Full=Docking protein 1; AltName: Full=Downstream of tyrosine kinase 1; AltName: Full=p62(dok);
FUNCTION: DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3 (By similarity).
SUBUNIT: Interacts with RasGAP, INPP5D/SHIP1 and ABL1. Interacts directly with phosphorylated ITGB3 (By similarity).
INTERACTION: P00520:Abl1; NbExp=4; IntAct=EBI-914917, EBI-914519; Q99M51:Nck1; NbExp=5; IntAct=EBI-914917, EBI-642202;
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
TISSUE SPECIFICITY: Expressed in lung, spleen, skeletal muscle and kidney.
DOMAIN: PTB domain mediates receptor interaction.
PTM: Constitutively tyrosine-phosphorylated. Phosphorylated by TEC.
PTM: Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway (By similarity). Phosphorylated by LYN.
DISRUPTION PHENOTYPE: No visible phenotype. Mice appear healthy and are fertile.
SIMILARITY: Belongs to the DOK family. Type A subfamily.
SIMILARITY: Contains 1 IRS-type PTB domain.
SIMILARITY: Contains 1 PH domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -17.0041-0.415 Picture PostScript Text
3' UTR -95.50317-0.301 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002404 - Insln_rcpt_S1
IPR011993 - PH_like_dom
IPR001849 - Pleckstrin_homology

Pfam Domains:
PF00169 - PH domain
PF02174 - PTB domain (IRS-1 type)

SCOP Domains:
50729 - PH domain-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1P5T - X-ray 1UEF - X-ray


ModBase Predicted Comparative 3D Structure on P97465
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
 RGDEnsembl   
 Protein Sequence    
 Alignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005057 signal transducer activity, downstream of receptor
GO:0005515 protein binding

Biological Process:
GO:0000165 MAPK cascade
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0007265 Ras protein signal transduction
GO:0035556 intracellular signal transduction

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  LF195602 - JP 2014500723-A/3105: Polycomb-Associated Non-Coding RNAs.
BC013066 - Mus musculus docking protein 1, mRNA (cDNA clone MGC:6661 IMAGE:3498170), complete cds.
LF204862 - JP 2014500723-A/12365: Polycomb-Associated Non-Coding RNAs.
AK136382 - Mus musculus 16 days embryo lung cDNA, RIKEN full-length enriched library, clone:8430417N18 product:downstream of tyrosine kinase 1, full insert sequence.
AK169871 - Mus musculus NOD-derived CD11c +ve dendritic cells cDNA, RIKEN full-length enriched library, clone:F630002C08 product:downstream of tyrosine kinase 1, full insert sequence.
U78818 - Mus musculus Abl- and p120 rasGAP-associated protein Dok (dok) mRNA, complete cds.
LF285137 - JP 2014500723-A/92640: Polycomb-Associated Non-Coding RNAs.
AK209408 - Mus musculus cDNA, clone:Y2G0115A09, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000000708, based on BLAT search.
LF285138 - JP 2014500723-A/92641: Polycomb-Associated Non-Coding RNAs.
LF202651 - JP 2014500723-A/10154: Polycomb-Associated Non-Coding RNAs.
LF203693 - JP 2014500723-A/11196: Polycomb-Associated Non-Coding RNAs.
AK180783 - Mus musculus cDNA, clone:Y0G0115N01, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000000708, based on BLAT search.
LF285139 - JP 2014500723-A/92642: Polycomb-Associated Non-Coding RNAs.
MA431179 - JP 2018138019-A/3105: Polycomb-Associated Non-Coding RNAs.
MA440439 - JP 2018138019-A/12365: Polycomb-Associated Non-Coding RNAs.
MA520714 - JP 2018138019-A/92640: Polycomb-Associated Non-Coding RNAs.
MA520715 - JP 2018138019-A/92641: Polycomb-Associated Non-Coding RNAs.
MA438228 - JP 2018138019-A/10154: Polycomb-Associated Non-Coding RNAs.
MA439270 - JP 2018138019-A/11196: Polycomb-Associated Non-Coding RNAs.
MA520716 - JP 2018138019-A/92642: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu05162 - Measles

Reactome (by CSHL, EBI, and GO)

Protein P97465 (Reactome details) participates in the following event(s):

R-MMU-8848774 PTK6 binds DOK1
R-MMU-8848776 PTK6 phosphorylates DOK1
R-MMU-8855617 2x p-5Y-RET:GDNF:GFRA complexes binds DOK1,DOK2,DOK4,DOK5,DOK6
R-MMU-8849469 PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1
R-MMU-8853659 RET signaling
R-MMU-8848021 Signaling by PTK6
R-MMU-422475 Axon guidance
R-MMU-9006927 Signaling by Non-Receptor Tyrosine Kinases
R-MMU-1266738 Developmental Biology
R-MMU-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: Dok, DOK1_MOUSE, NM_010070, P97465, Q9R213, uc009clr.1, uc009clr.2, uc009clr.3
UCSC ID: uc009clr.3
RefSeq Accession: NM_010070
Protein: P97465 (aka DOK1_MOUSE)
CCDS: CCDS20265.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.