Mouse Gene Nanog (ENSMUST00000112580.7) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
Description: Mus musculus Nanog homeobox (Nanog), transcript variant 3, mRNA. (from RefSeq NM_001289830) RefSeq Summary (NM_001289830): The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]. Gencode Transcript: ENSMUST00000112580.7 Gencode Gene: ENSMUSG00000012396.12 Transcript (Including UTRs) Position: mm10 chr6:122,707,565-122,714,633 Size: 7,069 Total Exon Count: 5 Strand: + Coding Region Position: mm10 chr6:122,707,858-122,713,555 Size: 5,698 Coding Exon Count: 4
ID:NANOG_MOUSE DESCRIPTION: RecName: Full=Homeobox protein NANOG; AltName: Full=ES cell-associated protein 4; AltName: Full=Early embryo specific expression NK-type homeobox protein; AltName: Full=Homeobox transcription factor Nanog; FUNCTION: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'- [CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation. SUBUNIT: Interacts with SMAD1 and SALL4. Interacts with ZNF281/ZFP281. INTERACTION: Q61066:Nr0b1; NbExp=6; IntAct=EBI-2312517, EBI-2312665; P20263:Pou5f1; NbExp=4; IntAct=EBI-2312517, EBI-1606219; Q6PR54:Rif1; NbExp=5; IntAct=EBI-2312517, EBI-2312621; P70414:Slc8a1; NbExp=9; IntAct=EBI-2312517, EBI-2312694; Q4FK52:Zfp281; NbExp=4; IntAct=EBI-2312517, EBI-2312719; SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Not expressed in oocytes and spermatogonia (at protein level). Not expressed in many somatic organs, ovary, testis, fibroblast and hematopoietic cell lines. DEVELOPMENTAL STAGE: Expressed in the central portion of the morula, the inner cell mass (ICM) of the blastocyst, in embryonic stem (ES) and embryonic germ (EG) cells, in the epiblast between 6.5 and 7.5 dpc, in primordial germ cells (PGCs) between 7.75 and 12.5 dpc (at protein level). The expression in PGCs decreases in female germ cells that entered meiosis at 13.5 dpc and in male germ cells that entered mitotic arrest at 14.5 dpc (at protein level). Not expressed in unfertilized eggs or 2- or 8-cell-stage embryos (at protein level). Expressed in the central portion of the morula, the inner cell mass (ICM) of the blastocyst, in ES and EG cells, in the epiblast at 6 dpc and in PGCs of genital ridges between 11.5 and 12.5 dpc. Expression decreases with ES differentiation. INDUCTION: By the transcription factor POU5F1 in ES cells that acts as a direct biphasic regulator: a steady-state concentration of POU5F1 up-regulated its expression, while an elevated concentration of POU5F1 down-regulated its expression. Up- regulated by the transcription factor FOXD3. Up-regulated in ES cells by transcription factors T (Brachyury) and STAT3. Down- regulated by p53 in response to DNA damage induced by ultraviolet light (UV) or doxorubicin. Down-regulated upon ES differentiation by mediating autorepression through interaction with ZNF281/ZFP281. DISRUPTION PHENOTYPE: Loss of pluripotency in both ICM and ES cells and differentiated into extraembryonic (parietal and visceral) endoderm lineage. MISCELLANEOUS: 'Nanog' is derived from 'Tir nan Og' the mythologic Celtic land of the ever young. SIMILARITY: Belongs to the Nanog homeobox family. SIMILARITY: Contains 1 homeobox DNA-binding domain.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q80Z64
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
US Server
