Mouse Gene Arhgef1 (ENSMUST00000098683.10) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus Rho guanine nucleotide exchange factor (GEF) 1 (Arhgef1), transcript variant 1, mRNA. (from RefSeq NM_001130150)
Gencode Transcript: ENSMUST00000098683.10
Gencode Gene: ENSMUSG00000040940.18
Transcript (Including UTRs)
   Position: mm10 chr7:24,903,051-24,926,589 Size: 23,539 Total Exon Count: 31 Strand: +
Coding Region
   Position: mm10 chr7:24,907,734-24,926,250 Size: 18,517 Coding Exon Count: 30 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:24,903,051-24,926,589)mRNA (may differ from genome)Protein (979 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: ARHG1_MOUSE
DESCRIPTION: RecName: Full=Rho guanine nucleotide exchange factor 1; AltName: Full=Lbc's second cousin; AltName: Full=Lymphoid blast crisis-like 2;
FUNCTION: Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2- induced RhoA activation. Isoform 3 and isoform 4 do not homooligomerize and show an enhanced RhoGEF activity.
SUBUNIT: Interacts with RHOA, GNA12 and GNA13 (By similarity). Homooligomerizes through the coiled coil region. Interacts with CTNNAL1 (By similarity). May interact with CCPG1.
INTERACTION: Q64727:Vcl; NbExp=3; IntAct=EBI-641821, EBI-432047;
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Membrane (By similarity). Note=Translocated to the membrane by activated GNA13 or LPA stimulation (By similarity).
TISSUE SPECIFICITY: Ubiquitously expressed.
DOMAIN: The RGSL domain, also known as rgRGS domain, is necessary but not sufficient for full GAP activity (By similarity).
DOMAIN: The DH domain is involved in interaction with CCPG1.
PTM: Phosphorylated by PKCA (By similarity). Angiotensin-2 induced Tyr-737 phosphorylation is mediated by JAK2. Isoform 5 is phosphorylated at 'Ser-390'.
DISRUPTION PHENOTYPE: Mice have reduced response to angiotensin-2 and lowered RhoA signaling pathway.
SIMILARITY: Contains 1 DH (DBL-homology) domain.
SIMILARITY: Contains 1 PH domain.
SIMILARITY: Contains 1 RGSL (RGS-like) domain.
CAUTION: Ref.2 sequence was originally submitted as from rat origin.

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

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Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -46.0096-0.479 Picture PostScript Text
3' UTR -116.10339-0.342 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000219 - DH-domain
IPR011993 - PH_like_dom
IPR001849 - Pleckstrin_homology
IPR015212 - Regulat_G_prot_signal-like
IPR016137 - Regulat_G_prot_signal_superfam
IPR015721 - RhoGEF-like

Pfam Domains:
PF00621 - RhoGEF domain
PF09128 - Regulator of G protein signalling-like domain

SCOP Domains:
48065 - DBL homology domain (DH-domain)
48097 - Regulator of G-protein signaling, RGS
50729 - PH domain-like

ModBase Predicted Comparative 3D Structure on Q61210
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
 RGD    
 Protein Sequence    
 Alignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001664 G-protein coupled receptor binding
GO:0005085 guanyl-nucleotide exchange factor activity
GO:0005089 Rho guanyl-nucleotide exchange factor activity
GO:0005096 GTPase activator activity
GO:0005515 protein binding

Biological Process:
GO:0007186 G-protein coupled receptor signaling pathway
GO:0035023 regulation of Rho protein signal transduction
GO:0043547 positive regulation of GTPase activity
GO:0060548 negative regulation of cell death

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0016020 membrane


-  Descriptions from all associated GenBank mRNAs
  AK043027 - Mus musculus 7 days neonate cerebellum cDNA, RIKEN full-length enriched library, clone:A730049B09 product:Rho guanine nucleotide exchange factor (GEF) 1, full insert sequence.
AK012156 - Mus musculus 10 days embryo whole body cDNA, RIKEN full-length enriched library, clone:2610528A06 product:Rho guanine nucleotide exchange factor (GEF) 1, full insert sequence.
AK051518 - Mus musculus 12 days embryo spinal ganglion cDNA, RIKEN full-length enriched library, clone:D130054G05 product:Rho guanine nucleotide exchange factor (GEF) 1, full insert sequence.
AK172354 - Mus musculus activated spleen cDNA, RIKEN full-length enriched library, clone:F830203O10 product:Rho guanine nucleotide exchange factor (GEF) 1, full insert sequence.
AK157056 - Mus musculus activated spleen cDNA, RIKEN full-length enriched library, clone:F830116B01 product:Rho guanine nucleotide exchange factor (GEF) 1, full insert sequence.
U58203 - Mus musculus Lsc (lsc) oncogene mRNA, complete cds.
AK162482 - Mus musculus 12 days embryo embryonic body between diaphragm region and neck cDNA, RIKEN full-length enriched library, clone:9430087G07 product:Rho guanine nucleotide exchange factor (GEF) 1, full insert sequence.
AK131181 - Mus musculus mRNA for mFLJ00369 protein.
AK040093 - Mus musculus 0 day neonate thymus cDNA, RIKEN full-length enriched library, clone:A430058L24 product:Rho guanine nucleotide exchange factor (GEF) 1, full insert sequence.
BC012488 - Mus musculus Rho guanine nucleotide exchange factor (GEF) 1, mRNA (cDNA clone MGC:11487 IMAGE:3154558), complete cds.
AK211978 - Mus musculus cDNA, clone:Y2G0123H15, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000047873, based on BLAT search.
AK199173 - Mus musculus cDNA, clone:Y1G0130G05, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000047873, based on BLAT search.
AK199940 - Mus musculus cDNA, clone:Y1G0132N11, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000047873, based on BLAT search.
AK197002 - Mus musculus cDNA, clone:Y1G0123K24, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000047873, based on BLAT search.
U89421 - Mus musculus strain BALB/c guanine nucleotide exchange factor Lsc (lsc) mRNA, partial cds.
AK201417 - Mus musculus cDNA, clone:Y1G0138D10, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000047873, based on BLAT search.
AK195489 - Mus musculus cDNA, clone:Y1G0118K11, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000047873, based on BLAT search.
AK199220 - Mus musculus cDNA, clone:Y1G0130I12, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000047873, based on BLAT search.
AY246272 - Mus musculus Rho guanine nucleotide exchange factor (Lsc) mRNA, partial cds; alternatively spliced.
AY246273 - Mus musculus Rho guanine nucleotide exchange factor (Lsc) mRNA, partial cds; alternatively spliced.
AK218306 - Mus musculus cDNA, clone:Y2G0144L20, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000047873, based on BLAT search.
AK203844 - Mus musculus cDNA, clone:Y1G0146G06, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000047873, based on BLAT search.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu04270 - Vascular smooth muscle contraction
mmu04810 - Regulation of actin cytoskeleton

BioCarta from NCI Cancer Genome Anatomy Project
m_Par1Pathway - Thrombin signaling and protease-activated receptors
m_rhoPathway - Rho cell motility signaling pathway

Reactome (by CSHL, EBI, and GO)

Protein Q61210 (Reactome details) participates in the following event(s):

R-MMU-114548 G alpha (13) activates Rho guanine nucleotide exchange factor 1 (p115-RhoGEF)
R-MMU-114544 p115-RhoGEF activation of Rac1
R-MMU-194913 GEFs activate Rho GTPase:GDP
R-MMU-419166 GEFs activate RhoA,B,C
R-MMU-205039 p75NTR indirectly activates RAC and Cdc42 via a guanyl-nucleotide exchange factor
R-MMU-416482 G alpha (12/13) signalling events
R-MMU-388396 GPCR downstream signalling
R-MMU-372790 Signaling by GPCR
R-MMU-194840 Rho GTPase cycle
R-MMU-193648 NRAGE signals death through JNK
R-MMU-162582 Signal Transduction
R-MMU-194315 Signaling by Rho GTPases
R-MMU-204998 Cell death signalling via NRAGE, NRIF and NADE
R-MMU-193704 p75 NTR receptor-mediated signalling
R-MMU-73887 Death Receptor Signalling

-  Other Names for This Gene
  Alternate Gene Symbols: ARHG1_MOUSE, Lbcl2, Lsc, NM_001130150, O89074, Q3T9Q7, Q61210, Q80YE8, Q80YE9, Q91VL3, uc009fqw.1, uc009fqw.2, uc009fqw.3, uc009fqw.4
UCSC ID: uc009fqw.4
RefSeq Accession: NM_001130150
Protein: Q61210 (aka ARHG1_MOUSE or ARH1_MOUSE)
CCDS: CCDS52140.1, CCDS52142.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.