Mouse Gene Klk1b3 (ENSMUST00000085450.3) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus kallikrein 1-related peptidase b3 (Klk1b3), mRNA. (from RefSeq NM_008693)
RefSeq Summary (NM_008693): This gene encodes the gamma subunit of the 7S nerve growth factor (NGF) complex that is essential for the differentiation and survival of distinct populations of neurons in both the central and the peripheral nervous systems. The encoded protein is a glandular kallikrein-type serine protease that processes the beta subunit of NGF. This gene is present in a cluster of multiple related kallikrein-type protease genes on chromosome 7. [provided by RefSeq, Jul 2015].
Gencode Transcript: ENSMUST00000085450.3
Gencode Gene: ENSMUSG00000066515.3
Transcript (Including UTRs)
   Position: mm10 chr7:44,198,191-44,202,352 Size: 4,162 Total Exon Count: 5 Strand: +
Coding Region
   Position: mm10 chr7:44,198,247-44,202,305 Size: 4,059 Coding Exon Count: 5 

Page IndexSequence and LinksUniProtKB CommentsPrimersRNA StructureProtein Structure
Other SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther NamesModel Information
Methods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:44,198,191-44,202,352)mRNA (may differ from genome)Protein (261 aa)
Gene SorterGenome BrowserOther Species FASTATable SchemaAlphaFoldBioGPS
EnsemblEntrez GeneExonPrimerGeneCardsMGIPubMed
SOURCEUniProtKB

-  Comments and Description Text from UniProtKB
  ID: K1KB3_MOUSE
DESCRIPTION: RecName: Full=Kallikrein 1-related peptidase b3; EC=3.4.21.35; AltName: Full=7S nerve growth factor gamma chain; AltName: Full=Gamma-NGF; AltName: Full=Glandular kallikrein K3; Short=mGK-3; AltName: Full=Tissue kallikrein-3; Contains: RecName: Full=Nerve growth factor gamma chain 1; Contains: RecName: Full=Nerve growth factor gamma chain 2; Flags: Precursor;
FUNCTION: 7S NGF alpha chain stabilizes the 7S complex. The beta dimer promotes neurite growth. The gamma chain is an arginine- specific protease; it may also have plasminogen activator activity, as well as mitogenic activity for chick embryo fibroblasts.
CATALYTIC ACTIVITY: Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
COFACTOR: Binds 2 zinc ions per 7S complex. The zinc ions are bound at the alpha-gamma interfaces.
SUBUNIT: 7S nerve growth factor is composed of two alpha chains, a beta dimer composed of identical chains, and two gamma chains.
MISCELLANEOUS: This precursor is cleaved into segments to produce the active form of the gamma chain, which occurs naturally as combinations of either two or three segments held together by disulfide bonds: B1 and A, or B1, C and B2.
SIMILARITY: Belongs to the peptidase S1 family. Kallikrein subfamily.
SIMILARITY: Contains 1 peptidase S1 domain.

-  Primer design for this transcript
 

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Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
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To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -16.8056-0.300 Picture PostScript Text
3' UTR -3.4047-0.072 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR009003 - Pept_cys/ser_Trypsin-like
IPR018114 - Peptidase_S1/S6_AS
IPR001254 - Peptidase_S1_S6
IPR001314 - Peptidase_S1A

Pfam Domains:
PF00089 - Trypsin
PF13365 - Trypsin-like peptidase domain

SCOP Domains:
50494 - Trypsin-like serine proteases

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1SGF - X-ray


ModBase Predicted Comparative 3D Structure on P00756
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
GenBank     
Protein Sequence     
Alignment     

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004175 endopeptidase activity
GO:0004252 serine-type endopeptidase activity
GO:0005057 signal transducer activity, downstream of receptor
GO:0008083 growth factor activity
GO:0008233 peptidase activity
GO:0008236 serine-type peptidase activity
GO:0016787 hydrolase activity
GO:0016811 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
GO:0046872 metal ion binding

Biological Process:
GO:0006508 proteolysis
GO:0007165 signal transduction
GO:0010469 regulation of receptor activity

Cellular Component:
GO:0005615 extracellular space
GO:0032991 macromolecular complex


-  Descriptions from all associated GenBank mRNAs
  BC132657 - Mus musculus kallikrein 1-related peptidase b3, mRNA (cDNA clone MGC:164288 IMAGE:40130934), complete cds.
BC132659 - Mus musculus kallikrein 1-related peptidase b3, mRNA (cDNA clone MGC:164290 IMAGE:40130936), complete cds.
X01389 - Mouse mRNA for gamma subunit of nerve growth factor (NGF).
X00472 - Mouse mRNA for gamma-7S nerve growth factor (y-NGF) fragment.
AK006516 - Mus musculus adult male testis cDNA, RIKEN full-length enriched library, clone:1700029O22 product:nerve growth factor, gamma, full insert sequence.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu04961 - Endocrine and other factor-regulated calcium reabsorption

BioCarta from NCI Cancer Genome Anatomy Project
m_cdk5Pathway - Phosphorylation of MEK1 by cdk5/p35 down regulates the MAP kinase pathway
m_ngfPathway - Nerve growth factor pathway (NGF)
m_trkaPathway - Trka Receptor Signaling Pathway
m_erkPathway - Erk1/Erk2 Mapk Signaling pathway

-  Other Names for This Gene
  Alternate Gene Symbols: A2RTW1, K1KB3_MOUSE, Klk-3, Klk3, Ngfg, NM_008693, P00756, uc009gol.1, uc009gol.2, uc009gol.3
UCSC ID: uc009gol.3
RefSeq Accession: NM_008693
Protein: P00756 (aka K1KB3_MOUSE)
CCDS: CCDS21199.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.