ID:EIF3F_MOUSE DESCRIPTION: RecName: Full=Eukaryotic translation initiation factor 3 subunit F; Short=eIF3f; AltName: Full=Deubiquitinating enzyme eIF3f; EC=3.4.19.12; AltName: Full=Eukaryotic translation initiation factor 3 subunit 5; AltName: Full=eIF-3-epsilon; AltName: Full=eIF3 p47; FUNCTION: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. FUNCTION: Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling (By similarity). CATALYTIC ACTIVITY: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C- terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). SUBUNIT: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex may interact with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation may lead to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with RNF139; the interaction leads to protein translation inhibitions in a ubiquitination-dependent manner. Interacts with DTX1, the interaction is required for deubiquitinating activity towards NOTCH1 (By similarity). SUBCELLULAR LOCATION: Cytoplasm (By similarity). DOMAIN: The MPN domain mediates deubiquitinating activity (By similarity). PTM: Phosphorylated. Phosphorylation is enhanced upon serum stimulation (By similarity). SIMILARITY: Belongs to the eIF-3 subunit F family. SIMILARITY: Contains 1 MPN (JAB/Mov34) domain.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF01398 - JAB1/Mov34/MPN/PAD-1 ubiquitin protease PF13012 - Maintenance of mitochondrial structure and function
ModBase Predicted Comparative 3D Structure on Q9DCH4
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AK152037 - Mus musculus bone marrow macrophage cDNA, RIKEN full-length enriched library, clone:I830045M21 product:eukaryotic translation initiation factor 3, subunit 5 (epsilon), full insert sequence. AK002778 - Mus musculus adult male kidney cDNA, RIKEN full-length enriched library, clone:0610037M02 product:eukaryotic translation initiation factor 3, subunit 5 (epsilon), full insert sequence. AK003372 - Mus musculus 18-day embryo whole body cDNA, RIKEN full-length enriched library, clone:1110003L04 product:eukaryotic translation initiation factor 3, subunit 5 (epsilon), full insert sequence. AK088537 - Mus musculus 2 days neonate thymus thymic cells cDNA, RIKEN full-length enriched library, clone:E430019L21 product:eukaryotic translation initiation factor 3, subunit 5 (epsilon), full insert sequence. AK136797 - Mus musculus adult male diencephalon cDNA, RIKEN full-length enriched library, clone:9330019A10 product:eukaryotic translation initiation factor 3, subunit 5 (epsilon), full insert sequence. AK010333 - Mus musculus ES cells cDNA, RIKEN full-length enriched library, clone:2410002E12 product:EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT 5 (EIF-3 EPSILON) (EIF3-P47) homolog [Homo sapiens], full insert sequence. BC070473 - Mus musculus eukaryotic translation initiation factor 3, subunit F, mRNA (cDNA clone MGC:99435 IMAGE:30634857), complete cds. BC083190 - Mus musculus eukaryotic translation initiation factor 3, subunit F, mRNA (cDNA clone MGC:90606 IMAGE:30358339), complete cds. BC050223 - Mus musculus, clone IMAGE:6430359, mRNA. AK193503 - Mus musculus cDNA, clone:Y1G0112I06, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. AK197956 - Mus musculus cDNA, clone:Y1G0126K21, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. AK190701 - Mus musculus cDNA, clone:Y1G0103I19, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. AK217928 - Mus musculus cDNA, clone:Y2G0143H12, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. AK192917 - Mus musculus cDNA, clone:Y1G0110F20, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. AK186458 - Mus musculus cDNA, clone:Y0G0137G11, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. AK200417 - Mus musculus cDNA, clone:Y1G0134H23, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. AK214434 - Mus musculus cDNA, clone:Y2G0131M01, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. AK209599 - Mus musculus cDNA, clone:Y2G0115J22, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. EF437360 - Mus musculus isolate NTA3K14 eukaryotic translation initiation factor 3 subunit 5 epsilon (Eif3s5) mRNA, partial cds. AY061808 - Mus musculus translation initiation factor-3 subunit 5 mRNA, partial cds. AK214157 - Mus musculus cDNA, clone:Y2G0130N14, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. AK207106 - Mus musculus cDNA, clone:Y2G0106P03, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. AK204915 - Mus musculus cDNA, clone:Y1G0150A14, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. AK201842 - Mus musculus cDNA, clone:Y1G0139I15, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. AK217304 - Mus musculus cDNA, clone:Y2G0141F14, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. AK190563 - Mus musculus cDNA, clone:Y1G0103C06, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. AK194257 - Mus musculus cDNA, clone:Y1G0114M05, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. AK187302 - Mus musculus cDNA, clone:Y0G0141C07, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. AK190312 - Mus musculus cDNA, clone:Y1G0102F24, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. AK194614 - Mus musculus cDNA, clone:Y1G0115O10, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. AK195637 - Mus musculus cDNA, clone:Y1G0119C08, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. AK198156 - Mus musculus cDNA, clone:Y1G0127E15, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. AK198457 - Mus musculus cDNA, clone:Y1G0128D21, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. AK211325 - Mus musculus cDNA, clone:Y2G0121G01, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. AK211674 - Mus musculus cDNA, clone:Y2G0122I02, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search. AK216628 - Mus musculus cDNA, clone:Y2G0139A01, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033342, based on BLAT search.
Biochemical and Signaling Pathways
KEGG - Kyoto Encyclopedia of Genes and Genomes mmu03013 - RNA transport
Reactome (by CSHL, EBI, and GO)
Protein Q9DCH4 (Reactome details) participates in the following event(s):
R-MMU-72676 eIF3 and eIF1A bind to the 40S subunit R-MMU-157849 Formation of translation initiation complexes containing mRNA that does not circularize R-MMU-72691 Formation of the 43S pre-initiation complex R-MMU-72621 Ribosomal scanning R-MMU-156823 Association of phospho-L13a with GAIT element of Ceruloplasmin mRNA R-MMU-72689 Formation of a pool of free 40S subunits R-MMU-72649 Translation initiation complex formation R-MMU-72695 Formation of the ternary complex, and subsequently, the 43S complex R-MMU-72737 Cap-dependent Translation Initiation R-MMU-72702 Ribosomal scanning and start codon recognition R-MMU-156827 L13a-mediated translation R-MMU-72662 Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S R-MMU-72613 Eukaryotic Translation Initiation R-MMU-72766 Translation R-MMU-392499 Metabolism of proteins