Mouse Gene Prkcb (ENSMUST00000064989.11) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus protein kinase C, beta (Prkcb), transcript variant 1, mRNA. (from RefSeq NM_008855)
Gencode Transcript: ENSMUST00000064989.11
Gencode Gene: ENSMUSG00000052889.11
Transcript (Including UTRs)
   Position: mm10 chr7:122,289,297-122,628,655 Size: 339,359 Total Exon Count: 17 Strand: +
Coding Region
   Position: mm10 chr7:122,289,345-122,627,814 Size: 338,470 Coding Exon Count: 17 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:122,289,297-122,628,655)mRNA (may differ from genome)Protein (673 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: KPCB_MOUSE
DESCRIPTION: RecName: Full=Protein kinase C beta type; Short=PKC-B; Short=PKC-beta; EC=2.7.11.13;
FUNCTION: Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1- MAPK/ERK signaling cascade. May participate in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity).
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain (By similarity).
ENZYME REGULATION: Classical (or conventional) PKCs (PRKCA, PRKCB and PRKCG) are activated by calcium and diacylglycerol (DAG) in the presence of phosphatidylserine. Three specific sites; Thr-500 (activation loop of the kinase domain), Thr-642 (turn motif) and Ser-661 (hydrophobic region), need to be phosphorylated for its full activation. Specifically inhibited by Enzastaurin (LY317615) (By similarity).
SUBUNIT: Interacts with PDK1. Interacts in vitro with PRKCBP1. Interacts with PHLPP1 and PHLPP2; both proteins mediate its dephosphorylation. Interacts with KDM1A/LSD1, PKN1 and ANDR (By similarity).
INTERACTION: Q13873:BMPR2 (xeno); NbExp=4; IntAct=EBI-397048, EBI-527196; P56537:EIF6 (xeno); NbExp=2; IntAct=EBI-397048, EBI-372243;
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Membrane; Peripheral membrane protein (By similarity).
PTM: Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity (By similarity). Phosphorylation at Tyr-662 by SYK induces binding with GRB2 and contributes to the activation of MAPK/ERK signaling cascade.
DISRUPTION PHENOTYPE: Mice develop an immunodeficiency characterized by impaired humoral immune responses and reduced cellular responses of B-cells similar to X-linked immunodeficiency (Xid). Mice are unable to activate NF-kappa-B and promote cell survival in B-cells upon BCR signaling, or even in mast cells. B- cells fail to recruit the I-kappa-B kinase (IKK) complex into lipid rafts, activate IKK, degrade I-kappa-B or up-regulate NF- kappa-B-dependent survival signals. Moreover, mutant animals are hyperphagic and exhibit higher food intake and reduced feed efficiency versus wild type. Mice are considerably leaner and display markedly decreased size of white fat depots. Triglyceride content in the liver and skeletal muscle is also significantly low.
SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
SIMILARITY: Contains 1 AGC-kinase C-terminal domain.
SIMILARITY: Contains 1 C2 domain.
SIMILARITY: Contains 2 phorbol-ester/DAG-type zinc fingers.
SIMILARITY: Contains 1 protein kinase domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -15.9048-0.331 Picture PostScript Text
3' UTR -269.00841-0.320 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000961 - AGC-kinase_C
IPR000008 - C2_Ca-dep
IPR008973 - C2_Ca/lipid-bd_dom_CaLB
IPR020477 - C2_dom
IPR018029 - C2_membr_targeting
IPR020454 - DAG/PE-bd
IPR011009 - Kinase-like_dom
IPR017892 - Pkinase_C
IPR002219 - Prot_Kinase_C-like_PE/DAG-bd
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR014375 - Protein_kinase_C_a/b/g
IPR002290 - Ser/Thr_dual-sp_kinase_dom
IPR008271 - Ser/Thr_kinase_AS

Pfam Domains:
PF00069 - Protein kinase domain
PF00130 - Phorbol esters/diacylglycerol binding domain (C1 domain)
PF00168 - C2 domain
PF00433 - Protein kinase C terminal domain
PF07714 - Protein tyrosine kinase

SCOP Domains:
49562 - C2 domain (Calcium/lipid-binding domain, CaLB)
56112 - Protein kinase-like (PK-like)
57889 - Cysteine-rich domain
57903 - FYVE/PHD zinc finger

ModBase Predicted Comparative 3D Structure on P68404
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
GenBankRGDEnsembl   
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003682 chromatin binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0004697 protein kinase C activity
GO:0005080 protein kinase C binding
GO:0005246 calcium channel regulator activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0030374 ligand-dependent nuclear receptor transcription coactivator activity
GO:0035403 histone kinase activity (H3-T6 specific)
GO:0042393 histone binding
GO:0046872 metal ion binding
GO:0050681 androgen receptor binding

Biological Process:
GO:0001666 response to hypoxia
GO:0002250 adaptive immune response
GO:0002376 immune system process
GO:0006325 chromatin organization
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0006468 protein phosphorylation
GO:0006816 calcium ion transport
GO:0006874 cellular calcium ion homeostasis
GO:0006915 apoptotic process
GO:0010829 negative regulation of glucose transport
GO:0014059 regulation of dopamine secretion
GO:0016310 phosphorylation
GO:0018105 peptidyl-serine phosphorylation
GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway
GO:0035408 histone H3-T6 phosphorylation
GO:0035556 intracellular signal transduction
GO:0040008 regulation of growth
GO:0042113 B cell activation
GO:0042493 response to drug
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB signaling
GO:0045766 positive regulation of angiogenesis
GO:0046627 negative regulation of insulin receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0050861 positive regulation of B cell receptor signaling pathway
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0071322 cellular response to carbohydrate stimulus

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005813 centrosome
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0031526 brush border membrane


-  Descriptions from all associated GenBank mRNAs
  AK051862 - Mus musculus 12 days embryo eyeball cDNA, RIKEN full-length enriched library, clone:D230012A21 product:protein kinase C, beta, full insert sequence.
X53532 - Mouse mRNA for protein kinase C beta-II.
BC127083 - Mus musculus protein kinase C, beta, mRNA (cDNA clone MGC:150300 IMAGE:40111744), complete cds.
X59274 - M.musculus mRNA brain protein kinase C beta-I.
AK034370 - Mus musculus adult male diencephalon cDNA, RIKEN full-length enriched library, clone:9330184D14 product:protein kinase C, beta, full insert sequence.
AK136923 - Mus musculus adult male diencephalon cDNA, RIKEN full-length enriched library, clone:9330188G12 product:protein kinase C, beta, full insert sequence.
AK088516 - Mus musculus 2 days neonate thymus thymic cells cDNA, RIKEN full-length enriched library, clone:E430019E24 product:protein kinase C, beta, full insert sequence.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu04010 - MAPK signaling pathway
mmu04012 - ErbB signaling pathway
mmu04020 - Calcium signaling pathway
mmu04062 - Chemokine signaling pathway
mmu04070 - Phosphatidylinositol signaling system
mmu04270 - Vascular smooth muscle contraction
mmu04310 - Wnt signaling pathway
mmu04370 - VEGF signaling pathway
mmu04510 - Focal adhesion
mmu04530 - Tight junction
mmu04540 - Gap junction
mmu04650 - Natural killer cell mediated cytotoxicity
mmu04662 - B cell receptor signaling pathway
mmu04664 - Fc epsilon RI signaling pathway
mmu04666 - Fc gamma R-mediated phagocytosis
mmu04670 - Leukocyte transendothelial migration
mmu04720 - Long-term potentiation
mmu04730 - Long-term depression
mmu04912 - GnRH signaling pathway
mmu04916 - Melanogenesis
mmu04960 - Aldosterone-regulated sodium reabsorption
mmu04961 - Endocrine and other factor-regulated calcium reabsorption
mmu04970 - Salivary secretion
mmu04971 - Gastric acid secretion
mmu04972 - Pancreatic secretion
mmu04973 - Carbohydrate digestion and absorption
mmu05140 - Leishmaniasis
mmu05143 - African trypanosomiasis
mmu05146 - Amoebiasis
mmu05200 - Pathways in cancer
mmu05214 - Glioma
mmu05223 - Non-small cell lung cancer

BioCarta from NCI Cancer Genome Anatomy Project
m_eif4Pathway - Regulation of eIF4e and p70 S6 Kinase
m_nos1Pathway - Nitric Oxide Signaling Pathway
m_pdgfPathway - PDGF Signaling Pathway
m_srcRPTPPathway - Activation of Src by Protein-tyrosine phosphatase alpha
m_tcrPathway - T Cell Receptor Signaling Pathway
m_cardiacegfPathway - Role of EGF Receptor Transactivation by GPCRs in Cardiac Hypertrophy
m_erbB4pathway - g-Secretase mediated ErbB4 Signaling Pathway
m_gpcrPathway - Signaling Pathway from G-Protein Families
m_fcer1Pathway - Fc Epsilon Receptor I Signaling in Mast Cells
m_bcrPathway - BCR Signaling Pathway
m_crebPathway - Transcription factor CREB and its extracellular signals
m_mef2dPathway - Role of MEF2D in T-cell Apoptosis
m_pkcPathway - Activation of PKC through G protein coupled receptor
m_agpcrPathway - Attenuation of GPCR Signaling
m_biopeptidesPathway - Bioactive Peptide Induced Signaling Pathway
m_calcineurinPathway - Effects of calcineurin in Keratinocyte Differentiation
m_cdMacPathway - Cadmium induces DNA synthesis and proliferation in macrophages
m_chemicalPathway - Apoptotic Signaling in Response to DNA Damage
m_cxcr4Pathway - CXCR4 Signaling Pathway
m_edg1Pathway - Phospholipids as signalling intermediaries
m_egfPathway - EGF Signaling Pathway
m_plcdPathway - Phospholipase C d1 in phospholipid associated cell signaling
m_arenrf2Pathway - Oxidative Stress Induced Gene Expression Via Nrf2
m_ghPathway - Growth Hormone Signaling Pathway
m_plcPathway - Phospholipase C Signaling Pathway
m_pyk2Pathway - Links between Pyk2 and Map Kinases
m_ionPathway - Ion Channel and Phorbal Esters Signaling Pathway
m_trkaPathway - Trka Receptor Signaling Pathway
m_CCR3Pathway - CCR3 signaling in Eosinophils
m_Ccr5Pathway - Pertussis toxin-insensitive CCR5 Signaling in Macrophage
m_keratinocytePathway - Keratinocyte Differentiation
m_cblPathway - CBL mediated ligand-induced downregulation of EGF receptors
m_At1rPathway - Angiotensin II mediated activation of JNK Pathway via Pyk2 dependent signaling
m_Par1Pathway - Thrombin signaling and protease-activated receptors
m_TPOPathway - TPO Signaling Pathway

Reactome (by CSHL, EBI, and GO)

Protein P68404 (Reactome details) participates in the following event(s):

R-MMU-1168373 PRKCB (Protein kinase C beta, PKC-beta) binds diacylglycerol and phosphatidylserine
R-MMU-114553 Activation of conventional Protein Kinase C
R-MMU-5218821 PDK1 phosphorylates PKC
R-MMU-5218805 PKC autophosphorylates
R-MMU-5218813 DAG and Ca+2 bind to PKC and tether it to membrane
R-MMU-5223304 DAG and Ca+2 bind to PKC and tether it to membrane
R-MMU-114683 Phosphorylation of Platelet Sec-1
R-MMU-114684 Phosphorylation of Syntaxin-4
R-MMU-416639 Trafficking of GluR2-containing AMPA receptors to extrasynaptic sites
R-MMU-421007 Endocytosis of Ca impermeable AMPA receptors
R-MMU-5138432 DVL2 is phosphorylated by PKC
R-MMU-5218823 PKC phosphorylates sphingosine kinase 1
R-MMU-1169091 Activation of NF-kappaB in B cells
R-MMU-76005 Response to elevated platelet cytosolic Ca2+
R-MMU-5218921 VEGFR2 mediated cell proliferation
R-MMU-1168372 Downstream signaling events of B Cell Receptor (BCR)
R-MMU-4419969 Depolymerisation of the Nuclear Lamina
R-MMU-76002 Platelet activation, signaling and aggregation
R-MMU-4420097 VEGFA-VEGFR2 Pathway
R-MMU-983705 Signaling by the B Cell Receptor (BCR)
R-MMU-2980766 Nuclear Envelope Breakdown
R-MMU-109582 Hemostasis
R-MMU-194138 Signaling by VEGF
R-MMU-1280218 Adaptive Immune System
R-MMU-114516 Disinhibition of SNARE formation
R-MMU-416993 Trafficking of GluR2-containing AMPA receptors
R-MMU-5099900 WNT5A-dependent internalization of FZD4
R-MMU-68875 Mitotic Prophase
R-MMU-9006934 Signaling by Receptor Tyrosine Kinases
R-MMU-168256 Immune System
R-MMU-399719 Trafficking of AMPA receptors
R-MMU-4086400 PCP/CE pathway
R-MMU-68886 M Phase
R-MMU-162582 Signal Transduction
R-MMU-399721 Glutamate binding, activation of AMPA receptors and synaptic plasticity
R-MMU-3858494 Beta-catenin independent WNT signaling
R-MMU-69278 Cell Cycle (Mitotic)
R-MMU-112314 Neurotransmitter receptors and postsynaptic signal transmission
R-MMU-195721 Signaling by WNT
R-MMU-1640170 Cell Cycle
R-MMU-112315 Transmission across Chemical Synapses
R-MMU-112316 Neuronal System

-  Other Names for This Gene
  Alternate Gene Symbols: A0JNZ5, F2Z441, KPCB_MOUSE, NM_008855, P04410, P04411, P68404, Pkcb, Prkcb1, uc009jou.1, uc009jou.2, uc009jou.3
UCSC ID: uc009jou.3
RefSeq Accession: NM_008855
Protein: P68404 (aka KPCB_MOUSE)
CCDS: CCDS21815.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.