Mouse Gene Gins4 (ENSMUST00000033950.6) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus GINS complex subunit 4 (Sld5 homolog) (Gins4), mRNA. (from RefSeq NM_024240)
Gencode Transcript: ENSMUST00000033950.6
Gencode Gene: ENSMUSG00000031546.6
Transcript (Including UTRs)
   Position: mm10 chr8:23,226,616-23,237,659 Size: 11,044 Total Exon Count: 8 Strand: -
Coding Region
   Position: mm10 chr8:23,227,079-23,237,075 Size: 9,997 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr8:23,226,616-23,237,659)mRNA (may differ from genome)Protein (223 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKB

-  Comments and Description Text from UniProtKB
  ID: SLD5_MOUSE
DESCRIPTION: RecName: Full=DNA replication complex GINS protein SLD5; AltName: Full=GINS complex subunit 4;
FUNCTION: The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS4 is important for GINS complex assembly. GINS complex seems to bind preferentially to single-stranded DNA (By similarity).
SUBUNIT: Component of the GINS complex which is a heterotetramer of GINS1, GINS2, GINS3 and GINS4. Forms a stable subcomplex with GINS1. GINS complex interacts with DNA primase in vitro (By similarity).
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
TISSUE SPECIFICITY: Highly abundant in testis. Weakly expressed in thymus and bone marrow.
SIMILARITY: Belongs to the GINS4/SLD5 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -88.50214-0.414 Picture PostScript Text
3' UTR -116.90463-0.252 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR021151 - GINS_complex
IPR008591 - GINS_Sld5

Pfam Domains:
PF05916 - GINS complex protein
PF16922 - DNA replication complex GINS protein SLD5 C-terminus

SCOP Domains:
158573 - GINS helical bundle-like
160059 - PriA/YqbF domain

ModBase Predicted Comparative 3D Structure on Q99LZ3
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologGenome BrowserGenome BrowserNo ortholog
Gene Details    Gene Details
Gene Sorter    Gene Sorter
GenBankRGDEnsemblEnsemblWormBaseSGD
Protein SequenceProtein Sequence Protein SequenceProtein Sequence 
AlignmentAlignment AlignmentAlignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0043138 3'-5' DNA helicase activity

Biological Process:
GO:0000727 double-strand break repair via break-induced replication
GO:0001833 inner cell mass cell proliferation
GO:0006260 DNA replication
GO:0006261 DNA-dependent DNA replication
GO:0006270 DNA replication initiation
GO:0032508 DNA duplex unwinding

Cellular Component:
GO:0000811 GINS complex
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0031298 replication fork protection complex


-  Descriptions from all associated GenBank mRNAs
  LF196845 - JP 2014500723-A/4348: Polycomb-Associated Non-Coding RNAs.
AK211204 - Mus musculus cDNA, clone:Y2G0120P19, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033950, based on BLAT search.
AK218577 - Mus musculus cDNA, clone:Y2G0145K02, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033950, based on BLAT search.
AK219168 - Mus musculus cDNA, clone:Y2G0147J12, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033950, based on BLAT search.
AK076104 - Mus musculus 10, 11 days embryo whole body cDNA, RIKEN full-length enriched library, clone:2810037C03 product:hypothetical protein, full insert sequence.
AK164815 - Mus musculus 16 days neonate heart cDNA, RIKEN full-length enriched library, clone:D830030P20 product:Sld5 homolog [Xenopus laevis], full insert sequence.
AK206391 - Mus musculus cDNA, clone:Y2G0104L08, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033950, based on BLAT search.
AK209294 - Mus musculus cDNA, clone:Y2G0114J24, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033950, based on BLAT search.
BC002156 - Mus musculus GINS complex subunit 4 (Sld5 homolog), mRNA (cDNA clone MGC:7298 IMAGE:3485425), complete cds.
LF298350 - JP 2014500723-A/105853: Polycomb-Associated Non-Coding RNAs.
AK016667 - Mus musculus adult male testis cDNA, RIKEN full-length enriched library, clone:4933405K01 product:hypothetical protein, full insert sequence.
AK177256 - Mus musculus cDNA, clone:Y0G0102C22, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000033950, based on BLAT search.
LF298351 - JP 2014500723-A/105854: Polycomb-Associated Non-Coding RNAs.
LF298355 - JP 2014500723-A/105858: Polycomb-Associated Non-Coding RNAs.
LF298356 - JP 2014500723-A/105859: Polycomb-Associated Non-Coding RNAs.
LF298357 - JP 2014500723-A/105860: Polycomb-Associated Non-Coding RNAs.
LF298358 - JP 2014500723-A/105861: Polycomb-Associated Non-Coding RNAs.
MA432422 - JP 2018138019-A/4348: Polycomb-Associated Non-Coding RNAs.
MA533927 - JP 2018138019-A/105853: Polycomb-Associated Non-Coding RNAs.
MA533928 - JP 2018138019-A/105854: Polycomb-Associated Non-Coding RNAs.
MA533932 - JP 2018138019-A/105858: Polycomb-Associated Non-Coding RNAs.
MA533933 - JP 2018138019-A/105859: Polycomb-Associated Non-Coding RNAs.
MA533934 - JP 2018138019-A/105860: Polycomb-Associated Non-Coding RNAs.
MA533935 - JP 2018138019-A/105861: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q99LZ3 (Reactome details) participates in the following event(s):

R-MMU-176956 Formation of GINS complex
R-MMU-176974 Unwinding of DNA
R-MMU-69190 DNA strand elongation
R-MMU-69239 Synthesis of DNA
R-MMU-69306 DNA Replication
R-MMU-69242 S Phase
R-MMU-69278 Cell Cycle (Mitotic)
R-MMU-1640170 Cell Cycle

-  Other Names for This Gene
  Alternate Gene Symbols: NM_024240, Q3TP06, Q99LZ3, Sld5, SLD5_MOUSE, uc009ler.1, uc009ler.2, uc009ler.3
UCSC ID: uc009ler.3
RefSeq Accession: NM_024240
Protein: Q99LZ3 (aka SLD5_MOUSE)
CCDS: CCDS22190.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.