Mouse Gene Alg9 (ENSMUST00000034561.10) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus asparagine-linked glycosylation 9 (alpha 1,2 mannosyltransferase) (Alg9), transcript variant 1, mRNA. (from RefSeq NM_133981)
Gencode Transcript: ENSMUST00000034561.10
Gencode Gene: ENSMUSG00000032059.13
Transcript (Including UTRs)
   Position: mm10 chr9:50,775,019-50,843,528 Size: 68,510 Total Exon Count: 15 Strand: +
Coding Region
   Position: mm10 chr9:50,775,385-50,842,857 Size: 67,473 Coding Exon Count: 15 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr9:50,775,019-50,843,528)mRNA (may differ from genome)Protein (611 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: ALG9_MOUSE
DESCRIPTION: RecName: Full=Alpha-1,2-mannosyltransferase ALG9; EC=2.4.1.259; EC=2.4.1.261; AltName: Full=Asparagine-linked glycosylation protein 9 homolog; AltName: Full=Disrupted in bipolar disorder protein 1 homolog; AltName: Full=Dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase; AltName: Full=Dol-P-Man:Man(8)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase;
FUNCTION: Catalyzes the transfer of mannose from Dol-P-Man to lipid-linked oligosaccharides (By similarity).
CATALYTIC ACTIVITY: Dolichyl beta-D-mannosyl phosphate + D-Man- alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha- (1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)- D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha- (1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->3)- D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D- GlcNAc-diphosphodolichol + dolichyl phosphate.
CATALYTIC ACTIVITY: Dolichyl beta-D-mannosyl phosphate + D-Man- alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha- (1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-alpha- (1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc- diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man- alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha- (1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)- D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.
PATHWAY: Protein modification; protein glycosylation.
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity).
SIMILARITY: Belongs to the glycosyltransferase 22 family.
SEQUENCE CAUTION: Sequence=BAC39717.1; Type=Frameshift; Positions=546;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -98.70366-0.270 Picture PostScript Text
3' UTR -200.90671-0.299 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR005599 - GPI_mannosylTrfase

Pfam Domains:
PF03901 - Alg9-like mannosyltransferase family

ModBase Predicted Comparative 3D Structure on Q8VDI9
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologGenome BrowserGenome BrowserNo ortholog
Gene Details    Gene Details
Gene Sorter    Gene Sorter
GenBankRGDEnsemblEnsemblWormBaseSGD
Protein SequenceProtein Sequence Protein SequenceProtein Sequence 
AlignmentAlignment AlignmentAlignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000030 mannosyltransferase activity
GO:0016740 transferase activity
GO:0016757 transferase activity, transferring glycosyl groups
GO:0052918 dol-P-Man:Man(8)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase activity
GO:0052926 dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase activity

Biological Process:
GO:0006486 protein glycosylation
GO:0097502 mannosylation

Cellular Component:
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  AK086674 - Mus musculus 15 days embryo head cDNA, RIKEN full-length enriched library, clone:D930045C02 product:SIMILAR TO DISRUPTED IN BIPOLAR DISORDER 1 homolog [Mus musculus], full insert sequence.
AK050335 - Mus musculus adult male liver tumor cDNA, RIKEN full-length enriched library, clone:C730038F19 product:DIBD1 homolog [Homo sapiens], full insert sequence.
AK149592 - Mus musculus adult male liver tumor cDNA, RIKEN full-length enriched library, clone:C730048I12 product:disrupted in bipolar disorder 1 homolog (human), full insert sequence.
AK054293 - Mus musculus 2 days pregnant adult female ovary cDNA, RIKEN full-length enriched library, clone:E330011F20 product:SIMILAR TO DISRUPTED IN BIPOLAR DISORDER 1 homolog [Mus musculus], full insert sequence.
BC021791 - Mus musculus asparagine-linked glycosylation 9 homolog (yeast, alpha 1,2 mannosyltransferase), mRNA (cDNA clone MGC:28731 IMAGE:4459738), complete cds.
AK214709 - Mus musculus cDNA, clone:Y2G0132L14, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000034561, based on BLAT search.
AK212096 - Mus musculus cDNA, clone:Y2G0123N14, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000034561, based on BLAT search.
AK189486 - Mus musculus cDNA, clone:Y0G0149F14, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000034561, based on BLAT search.
LF197591 - JP 2014500723-A/5094: Polycomb-Associated Non-Coding RNAs.
LF307008 - JP 2014500723-A/114511: Polycomb-Associated Non-Coding RNAs.
AK020231 - Mus musculus 15 days embryo embryonic body below diaphragm cDNA, RIKEN full-length enriched library, clone:8230402H15 product:DIBD1 homolog [Homo sapiens], full insert sequence.
AK201582 - Mus musculus cDNA, clone:Y1G0138L04, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000034561, based on BLAT search.
MA433168 - JP 2018138019-A/5094: Polycomb-Associated Non-Coding RNAs.
MA542585 - JP 2018138019-A/114511: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu00510 - N-Glycan biosynthesis
mmu01100 - Metabolic pathways

Reactome (by CSHL, EBI, and GO)

Protein Q8VDI9 (Reactome details) participates in the following event(s):

R-MMU-446215 ALG9 transfers Man to N-glycan precursor (GlcNAc)2 (Man)6 (PP-Dol)1
R-MMU-446216 ALG9 transfers Man to N-glycan precursor (GlcNAc)2 (Man)8 (PP-Dol)1
R-MMU-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
R-MMU-446203 Asparagine N-linked glycosylation
R-MMU-597592 Post-translational protein modification
R-MMU-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: ALG9_MOUSE, Dibd1, NM_133981, Q8BT44, Q8C378, Q8C7G0, Q8VDI9, uc009pkt.1, uc009pkt.2, uc009pkt.3
UCSC ID: uc009pkt.3
RefSeq Accession: NM_133981
Protein: Q8VDI9 (aka ALG9_MOUSE)
CCDS: CCDS23175.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.