Mouse Gene Dach2 (ENSMUST00000067219.4) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus dachshund family transcription factor 2 (Dach2), transcript variant 2, mRNA. (from RefSeq NM_033605)
Gencode Transcript: ENSMUST00000067219.4
Gencode Gene: ENSMUSG00000025592.17
Transcript (Including UTRs)
   Position: mm10 chrX:113,298,257-113,835,893 Size: 537,637 Total Exon Count: 13 Strand: +
Coding Region
   Position: mm10 chrX:113,298,427-113,835,448 Size: 537,022 Coding Exon Count: 13 

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Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther NamesModel Information
Methods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chrX:113,298,257-113,835,893)mRNA (may differ from genome)Protein (634 aa)
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-  Comments and Description Text from UniProtKB
  ID: DACH2_MOUSE
DESCRIPTION: RecName: Full=Dachshund homolog 2; Short=Dach2;
FUNCTION: Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator for SIX1 and SIX6. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Is recruited with SIX6 to the p27Kip1 promoter in embryonal retina. SIX6 corepression seems also to involve NCOR1, TBL1, HDAC1 and HDAC3. May be involved together with PAX3, SIX1, and EYA2 in regulation of myogenesis. In the developing somite, expression of DACH2 and PAX3 is regulated by the overlying ectoderm, and DACH2 and PAX3 positively regulate each other's expression. Probably binds to DNA via its DACHbox-N domain.
SUBUNIT: Interacts with SIX6. Interacts with EYA2 (By similarity).
SUBCELLULAR LOCATION: Nucleus (Probable).
TISSUE SPECIFICITY: Expressed in embryo, and at lower levels in the newborn.
DEVELOPMENTAL STAGE: From 8.5 to 14.5 dpc detected in nervous tissue. In the brain detected at 8.5 dpc within the prospective hindbrain, but not within the developing forebrain or midbrain. At 9.5 dpc expressed within the ventral prosencephalon, hindbrain and forebrain. Expression within the forebrain neuroectoderm flanked the optic vesicle with rostral and caudal restrictions. In addition, dorsal and ventral expression domains were observed within the hindbrain. At 10.5 to 12.5 dpc detected in the dorsal mesencephalon, in addition to the telencephalon and hindbrain. At 14.5 dpc visible in the olfactory bulbs. Detected from 9.5 to 12.5 dpc in the dorsal neural tube with the highest expression near the hindbrain. At 9.5 and 10.5 dpc detected in cells located in the dorsal and ventral neural tube and dorsal root ganglia. Detected during the development of the optic and the auditory systems. At 10.5 dpc, a small ring of ocular staining was observed suggesting expression in the lens pit. At 10.5 dpc expressed in the developing lens epithelium and in the mesenchyme surrounding the retina. However, expression in the lens placode ectoderm was not detected, suggesting that DACH2 is activated after lens vesicle formation. Low levels of expression could also be detected in the peripheral neuroretina at 10.5 and 12.5 dpc. Detected in the otic pit at 9.5 dpc and in the otic endolymphatic duct at 10.5, 11.5 and 12.5 dpc. From 10.5 to 14.5 dpc detected in the developing fore and hind limbs. At 10.5 and 11.5 dpc observed in the anterior and posterior margins and presumptive hand plate of the limb bud. At 9.5 and 10.5 dpc expressed in the limb. At 12.5 dpc is detected in the hand plate with strong expression at the margins of the limb plate. At 14.5 dpc detected in the hand plate and lateral edges of the digits. At 8.5 dpc expression was not detected in the developing somites. In contrast, from 9.5 to 12.5 dpc, expression is detected in a repeated pattern located lateral to the neural tube and in the interlimb bud region suggesting expression in somite derivatives. At 9.5 dpc detected in the forelimb dermamyotome. Also located along the lateral portion of the trunk and within a dorsal domain within the limb bud. At 10.5 dpc expressed in lateral and limb mesoderm. From 9.5 to 10.5 dpc detected in head mesenchyme and the branchial arches. At 9.5 and 10 dpc. expressed in the head mesoderm associated with the developing eye. At 10.5 dpc this pattern appears as a ring of expression surrounding the eye. At 11.5 dpc expression is still detectable in the branchial arches with strong expression at the cranial sinus. At 14.5 dpc mammary gland primordia. Detected in the nasal openings and vibrissae.
DOMAIN: The DACHbox-N domain forms a structure containing a DNA binding motif similar to that of the forkhead/winged helix domain (By similarity).
SIMILARITY: Belongs to the DACH/dachshund family.

-  Primer design for this transcript
 

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-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -42.60170-0.251 Picture PostScript Text
3' UTR -83.10445-0.187 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR009061 - DNA-bd_dom_put
IPR003380 - Transform_Ski

Pfam Domains:
PF02437 - SKI/SNO/DAC family

SCOP Domains:
46955 - Putative DNA-binding domain
52540 - P-loop containing nucleoside triphosphate hydrolases

ModBase Predicted Comparative 3D Structure on Q925Q8
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
GenBankRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001075 transcription factor activity, RNA polymerase II core promoter sequence-specific binding involved in preinitiation complex assembly
GO:0003677 DNA binding

Biological Process:
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0007275 multicellular organism development
GO:0046545 development of primary female sexual characteristics
GO:0051123 RNA polymerase II transcriptional preinitiation complex assembly

Cellular Component:
GO:0005634 nucleus
GO:0005667 transcription factor complex


-  Descriptions from all associated GenBank mRNAs
  AK033050 - Mus musculus adult male eyeball cDNA, RIKEN full-length enriched library, clone:7530414N05 product:similar to DACHSHUND-LIKE PROTEIN DACH2 [Mus musculus], full insert sequence.
AK041446 - Mus musculus 3 days neonate thymus cDNA, RIKEN full-length enriched library, clone:A630010A17 product:dachshund 2 (Drosophila), full insert sequence.
AK042401 - Mus musculus 3 days neonate thymus cDNA, RIKEN full-length enriched library, clone:A630088O16 product:dachshund 2 (Drosophila), full insert sequence.
AK045154 - Mus musculus 9.5 days embryo parthenogenote cDNA, RIKEN full-length enriched library, clone:B130041E18 product:dachshund 2 (Drosophila), full insert sequence.
AF257217 - Mus musculus dachshund-like protein DACH2 (Dach2) mRNA, complete cds.
BC059233 - Mus musculus dachshund 2 (Drosophila), mRNA (cDNA clone MGC:66847 IMAGE:6406445), complete cds.
GQ247884 - Mus musculus dachshund 2 variant 3 mRNA, partial cds, alternatively spliced.
AK038687 - Mus musculus adult male hypothalamus cDNA, RIKEN full-length enriched library, clone:A230055B21 product:dachshund 2 (Drosophila), full insert sequence.
MG576300 - UNVERIFIED: Mus musculus Dach2fiveprime mRNA, partial sequence.
MG576301 - UNVERIFIED: Mus musculus Dach2threeprime mRNA, partial sequence.

-  Other Names for This Gene
  Alternate Gene Symbols: DACH2_MOUSE, NM_033605, Q8BMA0, Q925Q8, uc009uds.1, uc009uds.2
UCSC ID: uc009uds.2
RefSeq Accession: NM_033605
Protein: Q925Q8 (aka DACH2_MOUSE or DAH2_MOUSE)
CCDS: CCDS30363.1, CCDS53175.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.