Mouse Gene Sh3kbp1 (ENSMUST00000080394.12) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus SH3-domain kinase binding protein 1 (Sh3kbp1), transcript variant 7, non-coding RNA. (from RefSeq NR_110970)
Gencode Transcript: ENSMUST00000080394.12
Gencode Gene: ENSMUSG00000040990.17
Transcript (Including UTRs)
   Position: mm10 chrX:159,708,601-159,974,167 Size: 265,567 Total Exon Count: 17 Strand: +
Coding Region
   Position: mm10 chrX:159,708,693-159,973,166 Size: 264,474 Coding Exon Count: 17 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chrX:159,708,601-159,974,167)mRNA (may differ from genome)Protein (628 aa)
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-  Comments and Description Text from UniProtKB
  ID: SH3K1_MOUSE
DESCRIPTION: RecName: Full=SH3 domain-containing kinase-binding protein 1; AltName: Full=Regulator of ubiquitous kinase; Short=Ruk; AltName: Full=SH3-containing, expressed in tumorigenic astrocytes;
FUNCTION: Adapter protein involved in regulating diverse signal transduction pathways. Involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through a association with CBL and endophilins. The association with CBL, and thus the receptor internalization, may inhibited by an interaction with PDCD6IP and/or SPRY2. Involved in regulation of ligand-dependent endocytosis of the IgE receptor. Attenuates phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit. May be involved in regulation of cell adhesion; promotes the interaction between TTK2B and PDCD6IP. May be involved in the regulation of cellular stress response via the MAPK pathways through its interaction with MAP3K4. Is involved in modulation of tumor necrosis factor mediated apoptosis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration (By similarity).
SUBUNIT: Can self-associate and form homotetramers. Interacts with CD2, F-actin capping protein, PIK3R3, GRB2, EGFR, MET, BLNK, MAP3K4, PDCD6IP, SPRY2, ARHGAP17, ARHGAP27, CRK, BCAR1, SOS1, ASAP1, ARAP3, HIP1R, SYNJ2, INPP5D and STAP1. Interacts with CBL and CBLB, but does not interact with CBLC. Two molecules of SH3KBP1 seem to bind through their respective SH3 1 domain to one molecule of CBLB. The interaction with CBL or CBLB and EGFR is increased upon EGF stimulation. The interaction with CBL is attenuated by PDCD6IP. Interacts through its proline-rich region with the SH3 domain of endophilins SH3GL1, SH3GL2 and SH3GL3. The SH3KBP1-endophilin complex seems to associate with a complex containing the phosphorylated receptor (EGFR or MET) and phosphorylated CBL. Probably associates with ASAP1 and phosphorylated EGFR. Probably part of a complex consisting of at least SH3KBP1, ASAP1 and ARAP3. Interacts with focal adhesion kinases PTK2/FAK1 AND PTK2B/PYK2, probably as a dimer. Interacts with DAB2 and probably associates with chathrin through its interaction with DAB2. Part of a complex consisting of SH3KBP1, DAB2, and clathrin heavy chain. DAB2 and clathrin dissociate from SH3KBP1 following growth factor treatment, enabling interaction with CBL. Interacts with DDN and probably associates with MAGI2 through its interaction with DDN. Interacts with the SH3 domains of SRC tyrosine-protein kinases SRC, LCK, LYN, FGR, FYN and HCK. Interacts with TRADD, BIRC2, TRAF1, TRAF2 and TNFR1, and the association with a TNFR1-associated complex upon stimulation with TNF-alpha seems to be mediated by SRC. Probably part of a complex consisting of at least SH3KBP1, ASAP1 and ARAP3 (By similarity). Interacts with SHKBP1. Interacts with ATX2.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton (By similarity). Cytoplasmic vesicle membrane; Peripheral membrane protein (By similarity). Cell junction, synapse, synaptosome (By similarity). Cell junction, focal adhesion (By similarity). Note=Localized in endocytic vesicles containing clustered receptors. Colocalizes with ASAP1 in vesicular structures. Colocalized with actin microfilaments and focal adhesions (By similarity). Colocalized with MAGI2 in synaptosomes (By similarity).
DOMAIN: The SH3 domains mediate interaction with SHKBP1.
PTM: Monoubiquitinated by CBL and CBLB after EGF stimulation; probably on its C-terminus (By similarity).
SIMILARITY: Contains 3 SH3 domains.

-  Primer design for this transcript
 

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-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -20.7092-0.225 Picture PostScript Text
3' UTR -236.201001-0.236 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000108 - p67phox
IPR011511 - SH3_2
IPR001452 - SH3_domain

Pfam Domains:
PF00018 - SH3 domain
PF07653 - Variant SH3 domain
PF14604 - Variant SH3 domain

SCOP Domains:
82061 - BAH domain
50044 - SH3-domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1WI7 - NMR 2DA9 - NMR


ModBase Predicted Comparative 3D Structure on Q8R550
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
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Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0017124 SH3 domain binding
GO:0031625 ubiquitin protein ligase binding

Biological Process:
GO:0006897 endocytosis
GO:0006915 apoptotic process
GO:0007010 cytoskeleton organization
GO:0008360 regulation of cell shape
GO:0016477 cell migration

Cellular Component:
GO:0005737 cytoplasm
GO:0005856 cytoskeleton
GO:0005911 cell-cell junction
GO:0005925 focal adhesion
GO:0016020 membrane
GO:0030054 cell junction
GO:0030139 endocytic vesicle
GO:0030659 cytoplasmic vesicle membrane
GO:0031410 cytoplasmic vesicle
GO:0043005 neuron projection
GO:0045202 synapse


-  Descriptions from all associated GenBank mRNAs
  AK049182 - Mus musculus ES cells cDNA, RIKEN full-length enriched library, clone:C330011K06 product:SH3-domain kinase binding protein 1, full insert sequence.
AK172022 - Mus musculus activated spleen cDNA, RIKEN full-length enriched library, clone:F830031B02 product:SH3-domain kinase binding protein 1, full insert sequence.
AK080930 - Mus musculus 4 days neonate male adipose cDNA, RIKEN full-length enriched library, clone:B430213G06 product:SH3-domain kinase binding protein 1, full insert sequence.
AK038540 - Mus musculus adult male hypothalamus cDNA, RIKEN full-length enriched library, clone:A230030G12 product:SH3 DOMAIN-CONTAINING ADAPTER PROTEIN ISOFORM SETA-1X23 (FRAGMENT) homolog [Rattus norvegicus], full insert sequence.
AK208632 - Mus musculus cDNA, clone:Y2G0112E02, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000050587, based on BLAT search.
BC034646 - Mus musculus cDNA clone IMAGE:5010913, containing frame-shift errors.
AK018032 - Mus musculus adult male thymus cDNA, RIKEN full-length enriched library, clone:5830464D22 product:SH3-domain kinase binding protein 1, full insert sequence.
AK030839 - Mus musculus adult male thymus cDNA, RIKEN full-length enriched library, clone:5830419C13 product:unclassifiable, full insert sequence.
AK004636 - Mus musculus adult male lung cDNA, RIKEN full-length enriched library, clone:1200007H22 product:SH3-domain kinase binding protein 1, full insert sequence.
AK020782 - Mus musculus 0 day neonate thymus cDNA, RIKEN full-length enriched library, clone:A430108A14 product:SH3-domain kinase binding protein 1, full insert sequence.
BC052863 - Mus musculus SH3-domain kinase binding protein 1, mRNA (cDNA clone IMAGE:30072744), containing frame-shift errors.
BC156605 - Synthetic construct Mus musculus clone IMAGE:100062095, MGC:190353 SH3-domain kinase binding protein 1 (Sh3kbp1) mRNA, encodes complete protein.
AK028299 - Mus musculus 17 days embryo head cDNA, RIKEN full-length enriched library, clone:3322401A22 product:SH3-domain kinase binding protein 1, full insert sequence.
AK161735 - Mus musculus adult male thymus cDNA, RIKEN full-length enriched library, clone:5830433B05 product:SH3-domain kinase binding protein 1, full insert sequence.
AK213880 - Mus musculus cDNA, clone:Y2G0129N05, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000054779, based on BLAT search.
AK161512 - Mus musculus adult male testis cDNA, RIKEN full-length enriched library, clone:4933428M15 product:SH3-domain kinase binding protein 1, full insert sequence.
AK007283 - Mus musculus adult male testis cDNA, RIKEN full-length enriched library, clone:1700125L08 product:SH3-domain kinase binding protein 1, full insert sequence.
AK142295 - Mus musculus 13 days embryo heart cDNA, RIKEN full-length enriched library, clone:D330037P03 product:SH3-domain kinase binding protein 1, full insert sequence.
AK052260 - Mus musculus 13 days embryo heart cDNA, RIKEN full-length enriched library, clone:D330014J07 product:SH3-domain kinase binding protein 1, full insert sequence.
AK198973 - Mus musculus cDNA, clone:Y1G0129L22, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000064453, based on BLAT search.
AK192695 - Mus musculus cDNA, clone:Y1G0109K13, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000064453, based on BLAT search.
AK190629 - Mus musculus cDNA, clone:Y1G0103F11, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000064453, based on BLAT search.
AK191921 - Mus musculus cDNA, clone:Y1G0107F14, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000064453, based on BLAT search.
AK197192 - Mus musculus cDNA, clone:Y1G0124F02, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000064453, based on BLAT search.
AK202551 - Mus musculus cDNA, clone:Y1G0141P07, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000064453, based on BLAT search.
AK196823 - Mus musculus cDNA, clone:Y1G0123B21, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000064453, based on BLAT search.
AK202593 - Mus musculus cDNA, clone:Y1G0142B17, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000064453, based on BLAT search.
AK208242 - Mus musculus cDNA, clone:Y2G0110I21, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000064453, based on BLAT search.
AK210647 - Mus musculus cDNA, clone:Y2G0119C03, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000064453, based on BLAT search.
AK217170 - Mus musculus cDNA, clone:Y2G0140O15, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000064453, based on BLAT search.
AK135598 - Mus musculus 1 day pregnant adult female ovary cDNA, RIKEN full-length enriched library, clone:7230402K07 product:SH3-domain kinase binding protein 1, full insert sequence.
AK200438 - Mus musculus cDNA, clone:Y1G0134J02, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000064453, based on BLAT search.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu04144 - Endocytosis

BioCarta from NCI Cancer Genome Anatomy Project
m_cblPathway - CBL mediated ligand-induced downregulation of EGF receptors

Reactome (by CSHL, EBI, and GO)

Protein Q8R550 (Reactome details) participates in the following event(s):

R-MMU-8855259 RELN:VLDLR:DAB1 binds SH3KBP1
R-MMU-983703 p-6Y-SYK phosphorylates BLNK (SLP65)
R-MMU-8855237 FYN phosphorylates DAB1 in RELN:VLDLR:DAB1:SH3KBP1
R-MMU-182994 Assembly of EGFR complex in clathrin-coated vesicles
R-MMU-183072 EGFR non-clathrin mediated endocytosis
R-MMU-8875482 CBL recruits CIN85:endophilin to ubiquitinated MET
R-MMU-8951490 CIN85 dissociates from EGF:p-6Y-EGFR:CBL:Beta-Pix:CDC42:GTP:CIN85
R-MMU-182986 CBL-mediated ubiquitination of CIN85
R-MMU-182990 Sprouty sequesters CBL away from active EGFR
R-MMU-183002 Beta-Pix:CDC42:GTP binds CBL in EGF:p-6Y-EGFR:CBL:CIN85
R-MMU-8867044 EGFR binds EPS15, EPN1, EPS15L1
R-MMU-8867041 EGFR phosphorylates EPS15
R-MMU-8867047 PTPN3 dephosphorylates EPS15
R-MMU-8866279 Epsin family proteins bind ubiquitinated cargo
R-MMU-8875490 EPS15 and HGS bind ubiquitinated MET
R-MMU-1112666 BLNK (SLP-65) Signalosome hydrolyzes phosphatidyinositol bisphosphate forming diacylglycerol and inositol-1,4,5-trisphosphate
R-MMU-8867756 CLASP proteins and cargo are recruited to the nascent clathrin-coated pit
R-MMU-8868071 Clathrin recruits PIK3C2A
R-MMU-8868661 Dynamin-mediated GTP hydrolysis promotes vesicle scission
R-MMU-8868648 SYNJ hydrolyze PI(4,5)P2 to PI(4)P
R-MMU-8868660 Auxilin recruits HSPA8:ATP to the clathrin-coated vesicle
R-MMU-8871194 RAB5 and GAPVD1 bind AP-2
R-MMU-8868659 Clathrin recruits auxilins to the clathrin-coated vesicle
R-MMU-8867754 F- and N- BAR domain proteins bind the clathrin-coated pit
R-MMU-8868230 SNX9 recruits components of the actin polymerizing machinery
R-MMU-8868072 Clathrin-associated PIK3C2A phosphorylates PI(4)P to PI(3,4)P2
R-MMU-8868236 BAR domain proteins recruit dynamin
R-MMU-8868651 Endophilins recruit synaptojanins to the clathrin-coated pit
R-MMU-8866376 Reelin signalling pathway
R-MMU-983695 Antigen activates B Cell Receptor (BCR) leading to generation of second messengers
R-MMU-422475 Axon guidance
R-MMU-182971 EGFR downregulation
R-MMU-6807004 Negative regulation of MET activity
R-MMU-983705 Signaling by the B Cell Receptor (BCR)
R-MMU-1266738 Developmental Biology
R-MMU-177929 Signaling by EGFR
R-MMU-6806834 Signaling by MET
R-MMU-1280218 Adaptive Immune System
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-9006934 Signaling by Receptor Tyrosine Kinases
R-MMU-168256 Immune System
R-MMU-8856828 Clathrin-mediated endocytosis
R-MMU-162582 Signal Transduction
R-MMU-199991 Membrane Trafficking
R-MMU-5653656 Vesicle-mediated transport

-  Other Names for This Gene
  Alternate Gene Symbols: B1AZ86, B1AZ87, NR_110970, Q8CAL8, Q8CEF6, Q8R545, Q8R546, Q8R547, Q8R548, Q8R549, Q8R550, Q8R551, Q9CTQ9, Q9DC14, Q9JKC3, Ruk, Seta, SH3K1_MOUSE, uc009usv.1, uc009usv.2, uc009usv.3, uc009usv.4
UCSC ID: uc009usv.4
RefSeq Accession: NM_021389
Protein: Q8R550 (aka SH3K1_MOUSE)
CCDS: CCDS41194.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.