Human Gene GFI1B (ENST00000372123.5) from GENCODE V44
Description: Homo sapiens growth factor independent 1B transcriptional repressor (GFI1B), transcript variant 5, mRNA. (from RefSeq NM_001377305) RefSeq Summary (NM_001135031): This gene encodes a zinc-finger containing transcriptional regulator that is primarily expressed in cells of hematopoietic lineage. The encoded protein complexes with numerous other transcriptional regulatory proteins including GATA-1, runt-related transcription factor 1 and histone deacetylases to control expression of genes involved in the development and maturation of erythrocytes and megakaryocytes. Mutations in this gene are the cause of the autosomal dominant platelet disorder, platelet-type bleeding disorder-17. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]. Gencode Transcript: ENST00000372123.5 Gencode Gene: ENSG00000165702.15 Transcript (Including UTRs) Position: hg38 chr9:132,978,711-132,991,687 Size: 12,977 Total Exon Count: 6 Strand: + Coding Region Position: hg38 chr9:132,986,679-132,991,050 Size: 4,372 Coding Exon Count: 5
ID:GFI1B_HUMAN DESCRIPTION: RecName: Full=Zinc finger protein Gfi-1b; AltName: Full=Growth factor independent protein 1B; AltName: Full=Potential regulator of CDKN1A translocated in CML; FUNCTION: Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Component of a RCOR-GFI-KDM1A-HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down- regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma- satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation. SUBUNIT: Component of a RCOR-GFI-KDM1A-HDAC complex. Interacts directly with RCOR1, KDM1A and HDAC2 (By similarity). Forms a complex with GATA1. Interacts with histone methyltransferases EHMT2 and SUV39H1. Interacts with ARIH2 (via RING-type 2). Interacts with RUNX1T1. INTERACTION: Q9HC77:CENPJ; NbExp=2; IntAct=EBI-946212, EBI-946194; Q9Y219:JAG2; NbExp=2; IntAct=EBI-946212, EBI-946223; Q99435:NELL2; NbExp=2; IntAct=EBI-946212, EBI-946274; SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Expressed in bone marrow and fetal liver, but also detectable in fetal spleen, fetal thymus, and testes. Detected in hematopoietic stem cells, erythroblasts, and megakaryocytes. Overexpressed in bone marrow of patients with erythroleukemia and megakaryocytic leukemia as well as in their corresponding leukemic cell lines, and markedly repressed in severe aplastic anemia (SAA). INDUCTION: By GATA1 which binds to GFI1B promoter in cooperation with the transcription factor NFYA. Target gene of transcription factor E2-alpha/TCF3 that promotes growth arrest and apoptosis in lymphomas. DOMAIN: The zinc finger domains are essential for erythroid expansion and acts as an activation domain whereas non finger domain serves as repression domain (By similarity). DOMAIN: The SNAG domain of GFIs is required for nuclear location and for interaction with some corepressors (By similarity). PTM: Methylation at Lys-8 in the SNAG domain seems required for the recruitment of the corepressor complex. SIMILARITY: Contains 6 C2H2-type zinc fingers.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q5VTD9
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding GO:0001085 RNA polymerase II transcription factor binding GO:0003676 nucleic acid binding GO:0003677 DNA binding GO:0005515 protein binding GO:0046872 metal ion binding
Biological Process: GO:0000122 negative regulation of transcription from RNA polymerase II promoter GO:0006325 chromatin organization GO:0006351 transcription, DNA-templated GO:0006355 regulation of transcription, DNA-templated GO:0006366 transcription from RNA polymerase II promoter GO:0007275 multicellular organism development GO:0008283 cell proliferation
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