Human Gene MRTFB (ENST00000573051.1) from GENCODE V44
  Description: Acts as a transcriptional coactivator of serum response factor (SRF). Required for skeletal myogenic differentiation. (from UniProt Q9ULH7)
Gencode Transcript: ENST00000573051.1
Gencode Gene: ENSG00000186260.17
Transcript (Including UTRs)
   Position: hg38 chr16:14,200,330-14,241,468 Size: 41,139 Total Exon Count: 9 Strand: +
Coding Region
   Position: hg38 chr16:14,200,488-14,240,825 Size: 40,338 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersRNA-Seq ExpressionMicroarray Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther Names
Methods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr16:14,200,330-14,241,468)mRNA (may differ from genome)Protein (378 aa)
Gene SorterGenome BrowserOther Species FASTATable SchemaAlphaFoldBioGPS
EnsemblExonPrimerGencodeGeneCardsHPRDMalacards
MGIneXtProtPubMedUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: MKL2_HUMAN
DESCRIPTION: RecName: Full=MKL/myocardin-like protein 2; AltName: Full=Megakaryoblastic leukemia 2; AltName: Full=Myocardin-related transcription factor B; Short=MRTF-B;
FUNCTION: Acts as a transcriptional coactivator of serum response factor (SRF). Required for skeletal myogenic differentiation.
SUBUNIT: Interacts with MKL1 and SRF.
INTERACTION: P11831:SRF; NbExp=3; IntAct=EBI-493007, EBI-493034;
SUBCELLULAR LOCATION: Nucleus (By similarity).
DOMAIN: The N-terminal region is required for nuclear localization and the C-terminal region mediates transcriptional activity (By similarity).
PTM: O-glycosylated (By similarity).
SIMILARITY: Contains 3 RPEL repeats.
SIMILARITY: Contains 1 SAP domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 14.68 RPKM in Brain - Frontal Cortex (BA9)
Total median expression: 352.99 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -35.00158-0.222 Picture PostScript Text
3' UTR -161.80643-0.252 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR004018 - RPEL_repeat
IPR003034 - SAP_DNA-bd

Pfam Domains:
PF02755 - RPEL repeat
PF02037 - SAP domain

ModBase Predicted Comparative 3D Structure on Q9ULH7
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGI     
Protein Sequence     
Alignment     

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001105 RNA polymerase II transcription coactivator activity
GO:0003713 transcription coactivator activity
GO:0005515 protein binding
GO:0045296 cadherin binding

Biological Process:
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006366 transcription from RNA polymerase II promoter
GO:0007275 multicellular organism development
GO:0007517 muscle organ development
GO:0030154 cell differentiation
GO:0045844 positive regulation of striated muscle tissue development
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0051145 smooth muscle cell differentiation

Cellular Component:
GO:0005634 nucleus


-  Descriptions from all associated GenBank mRNAs
  AK294765 - Homo sapiens cDNA FLJ61243 complete cds, highly similar to MKL/myocardin-like protein 2.
AY374297 - Homo sapiens megakaryoblastic leukemia 2 (MKL2) mRNA, complete cds.
BC034934 - Homo sapiens MKL/myocardin-like 2, mRNA (cDNA clone IMAGE:4907072), partial cds.
BC171750 - Homo sapiens MKL/myocardin-like 2, mRNA (cDNA clone MGC:198464 IMAGE:9053251), complete cds.
BC136260 - Homo sapiens MKL/myocardin-like 2, mRNA (cDNA clone MGC:167870 IMAGE:9020247), complete cds.
BC127941 - Homo sapiens MKL/myocardin-like 2, mRNA (cDNA clone IMAGE:40132674), partial cds.
AK093577 - Homo sapiens cDNA FLJ36258 fis, clone THYMU2002450.
AX748211 - Sequence 1736 from Patent EP1308459.
BC047761 - Homo sapiens MKL/myocardin-like 2, mRNA (cDNA clone IMAGE:5741919), complete cds.
LF326212 - JP 2014500723-A/133715: Polycomb-Associated Non-Coding RNAs.
MA561789 - JP 2018138019-A/133715: Polycomb-Associated Non-Coding RNAs.
CU690492 - Synthetic construct Homo sapiens gateway clone IMAGE:100022749 5' read MKL2 mRNA.
KJ902959 - Synthetic construct Homo sapiens clone ccsbBroadEn_12353 MKL2 gene, encodes complete protein.
JD547399 - Sequence 528423 from Patent EP1572962.
AB033069 - Homo sapiens mRNA for KIAA1243 protein, partial cds.

-  Other Names for This Gene
  Alternate Gene Symbols: A6ND53, BC047761, KIAA1243, MKL2, MKL2_HUMAN, Q68CT1, Q6UB16, Q86WW2, Q8N226, Q9ULH7, uc010uzb.1, uc010uzb.2, uc010uzb.3
UCSC ID: ENST00000573051.1
RefSeq Accession: NM_001365416
Protein: Q9ULH7 (aka MKL2_HUMAN)

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.