Human Gene NPEPPS (uc010wkt.2) Description and Page Index
  Description: Homo sapiens aminopeptidase puromycin sensitive (NPEPPS), mRNA.
RefSeq Summary (NM_006310): This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq, Jul 2008]. CCDS Note: Experimental evidence (N-terminal protein sequencing; PubMed ID 10978616) shows a peptide starting at Pro-46, suggesting that the downstream ATG (coding for Met-45) is used. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.63608.1, SRR1803612.124215.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000322157.9/ ENSP00000320324.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END##
Transcript (Including UTRs)
   Position: hg19 chr17:45,600,323-45,700,642 Size: 100,320 Total Exon Count: 24 Strand: +
Coding Region
   Position: hg19 chr17:45,600,353-45,699,286 Size: 98,934 Coding Exon Count: 24 

Page IndexSequence and LinksUniProtKB CommentsCTDGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr17:45,600,323-45,700,642)mRNA (may differ from genome)Protein (915 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
BioGPSCGAPEnsemblEntrez GeneExonPrimerGeneCards
PubMedStanford SOURCETreefamUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
DESCRIPTION: SubName: Full=Puromycin-sensitive aminopeptidase;
CAUTION: The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 47.82 RPKM in Esophagus - Mucosa
Total median expression: 1104.78 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -2.4030-0.080 Picture PostScript Text
3' UTR -355.581356-0.262 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR024571 - DUF3358
IPR001930 - Peptidase_M1
IPR014782 - Peptidase_M1_N
IPR015568 - Peptidase_M1_puromycin-sens

Pfam Domains:
PF01433 - Peptidase family M1 domain
PF11838 - ERAP1-like C-terminal domain

SCOP Domains:
63737 - Leukotriene A4 hydrolase N-terminal domain
55486 - Metalloproteases ("zincins"), catalytic domain

ModBase Predicted Comparative 3D Structure on E9PLK3
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004177 aminopeptidase activity
GO:0008233 peptidase activity
GO:0008237 metallopeptidase activity
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0046872 metal ion binding

Biological Process:
GO:0006508 proteolysis

Cellular Component:
GO:0005829 cytosol

-  Descriptions from all associated GenBank mRNAs
  AK303037 - Homo sapiens cDNA FLJ55506 complete cds, highly similar to Puromycin-sensitive aminopeptidase (EC3.4.11.-).
LF384798 - JP 2014500723-A/192301: Polycomb-Associated Non-Coding RNAs.
AK096709 - Homo sapiens cDNA FLJ39390 fis, clone PLACE6004219, highly similar to Puromycin-sensitive aminopeptidase (EC 3.4.11.-).
Y07701 - H.sapiens mRNA for aminopeptidase.
AK095877 - Homo sapiens cDNA FLJ38558 fis, clone HCHON2003327, highly similar to Puromycin-sensitive aminopeptidase (EC 3.4.11.-).
AK296887 - Homo sapiens cDNA FLJ53354 complete cds, highly similar to Puromycin-sensitive aminopeptidase (EC 3.4.11.-).
AJ132583 - Homo sapiens mRNA for puromycin sensitive aminopeptidase, partial.
BC065294 - Homo sapiens aminopeptidase puromycin sensitive, mRNA (cDNA clone MGC:70648 IMAGE:6059589), complete cds.
KJ901986 - Synthetic construct Homo sapiens clone ccsbBroadEn_11380 NPEPPS gene, encodes complete protein.
MA620375 - JP 2018138019-A/192301: Polycomb-Associated Non-Coding RNAs.
JD464720 - Sequence 445744 from Patent EP1572962.
JD150594 - Sequence 131618 from Patent EP1572962.
JD132789 - Sequence 113813 from Patent EP1572962.
JD418667 - Sequence 399691 from Patent EP1572962.
JD151807 - Sequence 132831 from Patent EP1572962.
AK297107 - Homo sapiens cDNA FLJ56108 complete cds, highly similar to Puromycin-sensitive aminopeptidase (EC 3.4.11.-).
AK311414 - Homo sapiens cDNA, FLJ18456.
LF327572 - JP 2014500723-A/135075: Polycomb-Associated Non-Coding RNAs.
LF327571 - JP 2014500723-A/135074: Polycomb-Associated Non-Coding RNAs.
AK293450 - Homo sapiens cDNA FLJ56052 complete cds, highly similar to Puromycin-sensitive aminopeptidase (EC 3.4.11.-).
AK293995 - Homo sapiens cDNA FLJ53310 complete cds, highly similar to Puromycin-sensitive aminopeptidase (EC 3.4.11.-).
LF327568 - JP 2014500723-A/135071: Polycomb-Associated Non-Coding RNAs.
JD023422 - Sequence 4446 from Patent EP1572962.
JD032913 - Sequence 13937 from Patent EP1572962.
LF327566 - JP 2014500723-A/135069: Polycomb-Associated Non-Coding RNAs.
JD023705 - Sequence 4729 from Patent EP1572962.
JD035567 - Sequence 16591 from Patent EP1572962.
AK096491 - Homo sapiens cDNA FLJ39172 fis, clone OCBBF2003091, highly similar to PUROMYCIN-SENSITIVE AMINOPEPTIDASE (EC 3.4.11.-).
LF327563 - JP 2014500723-A/135066: Polycomb-Associated Non-Coding RNAs.
LF327562 - JP 2014500723-A/135065: Polycomb-Associated Non-Coding RNAs.
LF327561 - JP 2014500723-A/135064: Polycomb-Associated Non-Coding RNAs.
LF327555 - JP 2014500723-A/135058: Polycomb-Associated Non-Coding RNAs.
LF327554 - JP 2014500723-A/135057: Polycomb-Associated Non-Coding RNAs.
LF327553 - JP 2014500723-A/135056: Polycomb-Associated Non-Coding RNAs.
JD498902 - Sequence 479926 from Patent EP1572962.
LF327551 - JP 2014500723-A/135054: Polycomb-Associated Non-Coding RNAs.
JD171035 - Sequence 152059 from Patent EP1572962.
JD443606 - Sequence 424630 from Patent EP1572962.
JD479554 - Sequence 460578 from Patent EP1572962.
LF327550 - JP 2014500723-A/135053: Polycomb-Associated Non-Coding RNAs.
JD483886 - Sequence 464910 from Patent EP1572962.
LF327549 - JP 2014500723-A/135052: Polycomb-Associated Non-Coding RNAs.
JD081717 - Sequence 62741 from Patent EP1572962.
JD555571 - Sequence 536595 from Patent EP1572962.
JD389235 - Sequence 370259 from Patent EP1572962.
JD076188 - Sequence 57212 from Patent EP1572962.
JD260010 - Sequence 241034 from Patent EP1572962.
JD133371 - Sequence 114395 from Patent EP1572962.
JD153823 - Sequence 134847 from Patent EP1572962.
JD153824 - Sequence 134848 from Patent EP1572962.
LF327548 - JP 2014500723-A/135051: Polycomb-Associated Non-Coding RNAs.
MA563149 - JP 2018138019-A/135075: Polycomb-Associated Non-Coding RNAs.
MA563148 - JP 2018138019-A/135074: Polycomb-Associated Non-Coding RNAs.
MA563145 - JP 2018138019-A/135071: Polycomb-Associated Non-Coding RNAs.
MA563143 - JP 2018138019-A/135069: Polycomb-Associated Non-Coding RNAs.
MA563140 - JP 2018138019-A/135066: Polycomb-Associated Non-Coding RNAs.
MA563139 - JP 2018138019-A/135065: Polycomb-Associated Non-Coding RNAs.
MA563138 - JP 2018138019-A/135064: Polycomb-Associated Non-Coding RNAs.
MA563132 - JP 2018138019-A/135058: Polycomb-Associated Non-Coding RNAs.
MA563131 - JP 2018138019-A/135057: Polycomb-Associated Non-Coding RNAs.
MA563130 - JP 2018138019-A/135056: Polycomb-Associated Non-Coding RNAs.
MA563128 - JP 2018138019-A/135054: Polycomb-Associated Non-Coding RNAs.
MA563127 - JP 2018138019-A/135053: Polycomb-Associated Non-Coding RNAs.
MA563126 - JP 2018138019-A/135052: Polycomb-Associated Non-Coding RNAs.
MA563125 - JP 2018138019-A/135051: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: AK303037, E9PLK3, E9PLK3_HUMAN, NM_006310, NP_006301
UCSC ID: uc010wkt.2
RefSeq Accession: NM_006310
Protein: E9PLK3 CCDS: CCDS45721.1

-  Gene Model Information
category: coding nonsense-mediated-decay: no RNA accession: AK303037.1
exon count: 24CDS single in 3' UTR: no RNA size: 2864
ORF size: 2748CDS single in intron: no Alignment % ID: 99.93
txCdsPredict score: 5530.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.