Human Gene NMRK2 (ENST00000616156.4) from GENCODE V44
  Description: Homo sapiens nicotinamide riboside kinase 2 (NMRK2), transcript variant 1, mRNA. (from RefSeq NM_001289117)
Gencode Transcript: ENST00000616156.4
Gencode Gene: ENSG00000077009.14
Transcript (Including UTRs)
   Position: hg38 chr19:3,933,103-3,942,414 Size: 9,312 Total Exon Count: 8 Strand: +
Coding Region
   Position: hg38 chr19:3,933,672-3,942,273 Size: 8,602 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:3,933,103-3,942,414)mRNA (may differ from genome)Protein (235 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
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HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: NRK2_HUMAN
DESCRIPTION: RecName: Full=Nicotinamide riboside kinase 2; Short=NRK 2; Short=NmR-K 2; EC=2.7.1.22; AltName: Full=Integrin beta-1-binding protein 3; AltName: Full=Muscle integrin-binding protein; Short=MIBP; AltName: Full=Nicotinic acid riboside kinase 2; EC=2.7.1.173; AltName: Full=Ribosylnicotinamide kinase 2; Short=RNK 2; AltName: Full=Ribosylnicotinic acid kinase 2;
FUNCTION: Catalyzes the phosphorylation of nicotinamide riboside (NR) and nicotinic acid riboside (NaR) to form nicotinamide mononucleotide (NMN) and nicotinic acid mononucleotide (NaMN). Reduces laminin matrix deposition and cell adhesion to laminin, but not to fibronectin. Involved in the regulation of PXN at the protein level and of PXN tyrosine phosphorylation. May play a role in the regulation of terminal myogenesis.
CATALYTIC ACTIVITY: ATP + 1-(beta-D-ribofuranosyl)-nicotinamide = ADP + beta-nicotinamide D-ribonucleotide.
CATALYTIC ACTIVITY: ATP + beta-D-ribosylnicotinate = ADP + nicotinate beta-D-ribonucleotide.
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.19 mM for nicotinamide riboside (with ATP as cosubstrate); KM=30 mM for nicotinamide riboside (with GTP as cosubstrate); KM=0.11 mM for tiazofurin (with ATP as cosubstrate); KM=0.063 mM for nicotinic acid riboside (with ATP as cosubstrate); KM=1.3 mM for uridine (with ATP as cosubstrate);
PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis.
SUBUNIT: Monomer (By similarity). Interacts with ITGB1 alone or when associated with alpha-7, but not with alpha-5.
INTERACTION: Q9Y561:LRP12; NbExp=2; IntAct=EBI-514059, EBI-296693;
TISSUE SPECIFICITY: Predominantly expressed in skeletal muscle and, at a much lower level, in the heart (at protein level). No expression in brain, kidney, liver, lung, pancreas nor placenta.
INDUCTION: Down-regulated during myoblast differentiation (By similarity).
SIMILARITY: Belongs to the uridine kinase family. NRK subfamily.
SEQUENCE CAUTION: Sequence=AAF26711.1; Type=Miscellaneous discrepancy; Note=Aberrant splicing;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 601.97 RPKM in Heart - Atrial Appendage
Total median expression: 1133.59 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -138.70290-0.478 Picture PostScript Text
3' UTR -53.90141-0.382 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  ModBase Predicted Comparative 3D Structure on Q9NPI5
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0046872 metal ion binding
GO:0050262 ribosylnicotinamide kinase activity
GO:0061769 ribosylnicotinate kinase activity

Biological Process:
GO:0009435 NAD biosynthetic process
GO:0016310 phosphorylation
GO:0019363 pyridine nucleotide biosynthetic process
GO:0019674 NAD metabolic process
GO:0045662 negative regulation of myoblast differentiation

Cellular Component:
GO:0005622 intracellular
GO:0005654 nucleoplasm
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0043231 intracellular membrane-bounded organelle


-  Descriptions from all associated GenBank mRNAs
  AK001663 - Homo sapiens cDNA FLJ10801 fis, clone NT2RP4000781.
JD211275 - Sequence 192299 from Patent EP1572962.
AK022514 - Homo sapiens cDNA FLJ12452 fis, clone NT2RM1000388.
AF190819 - Homo sapiens muscle beta 1 intergrin cytoplasmic domain binding protein MIBP (MIBP) mRNA, complete cds.
AL365377 - Homo sapiens mRNA full length insert cDNA clone EUROIMAGE 1846965.
BC093637 - Homo sapiens integrin beta 1 binding protein 3, mRNA (cDNA clone MGC:120672 IMAGE:7939482), complete cds.
BC101575 - Homo sapiens integrin beta 1 binding protein 3, mRNA (cDNA clone MGC:126624 IMAGE:8069081), complete cds.
BC143329 - Homo sapiens integrin beta 1 binding protein 3, mRNA (cDNA clone MGC:176851 IMAGE:9051834), complete cds.
KJ905455 - Synthetic construct Homo sapiens clone ccsbBroadEn_15042 ITGB1BP3 gene, encodes complete protein.
AY611481 - Homo sapiens nicotinamide riboside kinase 2 (NRK2) mRNA, complete cds.
JD252693 - Sequence 233717 from Patent EP1572962.
JD348663 - Sequence 329687 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9NPI5 (Reactome details) participates in the following event(s):

R-HSA-8869627 NMRK2 phosphorylates NR to yield NMN
R-HSA-8869607 NMRK2 phosphorylates NAR to yield NAMN
R-HSA-196807 Nicotinate metabolism
R-HSA-196849 Metabolism of water-soluble vitamins and cofactors
R-HSA-196854 Metabolism of vitamins and cofactors
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000616156.1, ENST00000616156.2, ENST00000616156.3, ITGB1BP3, NM_001289117, NRK2, NRK2_HUMAN, Q52M81, Q9NPI5, Q9NZK3, uc010xia.1, uc010xia.2, uc010xia.3, uc010xia.4
UCSC ID: ENST00000616156.4
RefSeq Accession: NM_001289117
Protein: Q9NPI5 (aka NRK2_HUMAN)
CCDS: CCDS12115.1, CCDS74259.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.