ID:NRK2_HUMAN DESCRIPTION: RecName: Full=Nicotinamide riboside kinase 2; Short=NRK 2; Short=NmR-K 2; EC=2.7.1.22; AltName: Full=Integrin beta-1-binding protein 3; AltName: Full=Muscle integrin-binding protein; Short=MIBP; AltName: Full=Nicotinic acid riboside kinase 2; EC=2.7.1.173; AltName: Full=Ribosylnicotinamide kinase 2; Short=RNK 2; AltName: Full=Ribosylnicotinic acid kinase 2; FUNCTION: Catalyzes the phosphorylation of nicotinamide riboside (NR) and nicotinic acid riboside (NaR) to form nicotinamide mononucleotide (NMN) and nicotinic acid mononucleotide (NaMN). Reduces laminin matrix deposition and cell adhesion to laminin, but not to fibronectin. Involved in the regulation of PXN at the protein level and of PXN tyrosine phosphorylation. May play a role in the regulation of terminal myogenesis. CATALYTIC ACTIVITY: ATP + 1-(beta-D-ribofuranosyl)-nicotinamide = ADP + beta-nicotinamide D-ribonucleotide. CATALYTIC ACTIVITY: ATP + beta-D-ribosylnicotinate = ADP + nicotinate beta-D-ribonucleotide. BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.19 mM for nicotinamide riboside (with ATP as cosubstrate); KM=30 mM for nicotinamide riboside (with GTP as cosubstrate); KM=0.11 mM for tiazofurin (with ATP as cosubstrate); KM=0.063 mM for nicotinic acid riboside (with ATP as cosubstrate); KM=1.3 mM for uridine (with ATP as cosubstrate); PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis. SUBUNIT: Monomer (By similarity). Interacts with ITGB1 alone or when associated with alpha-7, but not with alpha-5. INTERACTION: Q9Y561:LRP12; NbExp=2; IntAct=EBI-514059, EBI-296693; TISSUE SPECIFICITY: Predominantly expressed in skeletal muscle and, at a much lower level, in the heart (at protein level). No expression in brain, kidney, liver, lung, pancreas nor placenta. INDUCTION: Down-regulated during myoblast differentiation (By similarity). SIMILARITY: Belongs to the uridine kinase family. NRK subfamily. SEQUENCE CAUTION: Sequence=AAF26711.1; Type=Miscellaneous discrepancy; Note=Aberrant splicing;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Protein Domain and Structure Information
ModBase Predicted Comparative 3D Structure on Q9NPI5
Front
Top
Side
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0009435 NAD biosynthetic process GO:0016310 phosphorylation GO:0019363 pyridine nucleotide biosynthetic process GO:0019674 NAD metabolic process GO:0045662 negative regulation of myoblast differentiation