Human Gene GUCY1B1 (ENST00000505764.5) from GENCODE V44
  Description: Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1), transcript variant 3, mRNA. (from RefSeq NM_001291952)
RefSeq Summary (NM_001291952): This gene encodes the beta subunit of the soluble guanylate cyclase (sGC), which catalyzes the conversion of GTP (guanosine triphosphate) to cGMP (cyclic guanosine monophosphate). The encoded protein contains an HNOX domain, which serves as a receptor for ligands such as nitric oxide, oxygen and nitrovasodilator drugs. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014].
Gencode Transcript: ENST00000505764.5
Gencode Gene: ENSG00000061918.14
Transcript (Including UTRs)
   Position: hg38 chr4:155,759,026-155,806,738 Size: 47,713 Total Exon Count: 15 Strand: +
Coding Region
   Position: hg38 chr4:155,772,737-155,806,409 Size: 33,673 Coding Exon Count: 13 

Page IndexSequence and LinksUniProtKB CommentsPrimersRNA-Seq ExpressionMicroarray Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathways
Other NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr4:155,759,026-155,806,738)mRNA (may differ from genome)Protein (599 aa)
Gene SorterGenome BrowserOther Species FASTATable SchemaAlphaFoldBioGPS
EnsemblEntrez GeneExonPrimerGencodeGeneCardsHPRD
LynxMalacardsMGIneXtProtOMIMPubMed
ReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: GCYB1_HUMAN
DESCRIPTION: RecName: Full=Guanylate cyclase soluble subunit beta-1; Short=GCS-beta-1; EC=4.6.1.2; AltName: Full=Guanylate cyclase soluble subunit beta-3; Short=GCS-beta-3; AltName: Full=Soluble guanylate cyclase small subunit;
CATALYTIC ACTIVITY: GTP = 3',5'-cyclic GMP + diphosphate.
COFACTOR: Binds 1 or 2 heme groups per heterodimer (By similarity).
ENZYME REGULATION: Activated by nitric oxide in the presence of magnesium or manganese ions.
SUBUNIT: Heterodimer of an alpha and a beta chain.
SUBCELLULAR LOCATION: Cytoplasm.
MISCELLANEOUS: There are two types of guanylate cyclases: soluble forms and membrane-associated receptor forms.
SIMILARITY: Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
SIMILARITY: Contains 1 guanylate cyclase domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 35.38 RPKM in Brain - Frontal Cortex (BA9)
Total median expression: 490.54 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -119.90327-0.367 Picture PostScript Text
3' UTR -77.60329-0.236 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001054 - A/G_cyclase
IPR018297 - A/G_cyclase_CS
IPR011645 - Haem_no_assoc-bd
IPR011644 - Heme_NO-bd
IPR024096 - NO_sig/Golgi_transp_ligand-bd

Pfam Domains:
PF00211 - Adenylate and Guanylate cyclase catalytic domain
PF07700 - Haem-NO-binding
PF07701 - Heme NO binding associated

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2WZ1 - X-ray MuPIT 3UVJ - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q02153
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologGenome BrowserNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD Ensembl  
Protein SequenceProtein Sequence Protein Sequence  
AlignmentAlignment Alignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004383 guanylate cyclase activity
GO:0005515 protein binding
GO:0005525 GTP binding
GO:0016829 lyase activity
GO:0016849 phosphorus-oxygen lyase activity
GO:0020037 heme binding
GO:0038023 signaling receptor activity
GO:0046872 metal ion binding
GO:0004016 adenylate cyclase activity

Biological Process:
GO:0006182 cGMP biosynthetic process
GO:0007263 nitric oxide mediated signal transduction
GO:0008015 blood circulation
GO:0009190 cyclic nucleotide biosynthetic process
GO:0035556 intracellular signal transduction
GO:0038060 nitric oxide-cGMP-mediated signaling pathway
GO:0071732 cellular response to nitric oxide

Cellular Component:
GO:0005737 cytoplasm
GO:0005886 plasma membrane
GO:0008074 guanylate cyclase complex, soluble


-  Descriptions from all associated GenBank mRNAs
  AK315944 - Homo sapiens cDNA, FLJ78843 complete cds, highly similar to Guanylate cyclase soluble subunit beta-1 (EC4.6.1.2).
AK315199 - Homo sapiens cDNA, FLJ96185, highly similar to Homo sapiens guanylate cyclase 1, soluble, beta 3 (GUCY1B3), mRNA.
AK300296 - Homo sapiens cDNA FLJ53919 complete cds, highly similar to Guanylate cyclase soluble subunit beta-1 (EC 4.6.1.2).
AK296680 - Homo sapiens cDNA FLJ59711 complete cds, highly similar to Guanylate cyclase soluble subunit beta-1 (EC 4.6.1.2).
AK307838 - Homo sapiens cDNA, FLJ97786.
BC026332 - Homo sapiens cDNA clone IMAGE:4794254, containing frame-shift errors.
BC060835 - Homo sapiens cDNA clone IMAGE:5312528, containing frame-shift errors.
JD124836 - Sequence 105860 from Patent EP1572962.
BC047620 - Homo sapiens guanylate cyclase 1, soluble, beta 3, mRNA (cDNA clone MGC:51012 IMAGE:5264160), complete cds.
X66533 - H.sapiens soluble guanylate cyclase small subunit mRNA.
AF020340 - Homo sapiens soluble guanylate cyclase beta-1 subunit (GC-S-beta-1) mRNA, alternatively spliced, complete cds.
JD144460 - Sequence 125484 from Patent EP1572962.
AK299916 - Homo sapiens cDNA FLJ50853 complete cds, highly similar to Guanylate cyclase soluble subunit beta-1 (EC 4.6.1.2).
CU690442 - Synthetic construct Homo sapiens gateway clone IMAGE:100020967 5' read GUCY1B3 mRNA.
AB527415 - Synthetic construct DNA, clone: pF1KB4146, Homo sapiens GUCY1B3 gene for guanylate cyclase 1, soluble, beta 3, without stop codon, in Flexi system.
KJ896949 - Synthetic construct Homo sapiens clone ccsbBroadEn_06343 GUCY1B3 gene, encodes complete protein.
JD267741 - Sequence 248765 from Patent EP1572962.
JD109859 - Sequence 90883 from Patent EP1572962.
JD251692 - Sequence 232716 from Patent EP1572962.
JD412062 - Sequence 393086 from Patent EP1572962.
JD345787 - Sequence 326811 from Patent EP1572962.
JD241746 - Sequence 222770 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00230 - Purine metabolism
hsa04270 - Vascular smooth muscle contraction
hsa04540 - Gap junction
hsa04730 - Long-term depression

BioCarta from NCI Cancer Genome Anatomy Project
h_raccPathway - Ion Channels and Their Functional Role in Vascular Endothelium

Reactome (by CSHL, EBI, and GO)

Protein Q02153 (Reactome details) participates in the following event(s):

R-HSA-392143 NO binds to Guanylate Cyclase
R-HSA-392152 Soluble guanylate cyclase converts GTP to cGMP
R-HSA-392154 Nitric oxide stimulates guanylate cyclase
R-HSA-418346 Platelet homeostasis
R-HSA-109582 Hemostasis

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000505764.1, ENST00000505764.2, ENST00000505764.3, ENST00000505764.4, GCYB1_HUMAN, GUC1B3, GUCSB3, GUCY1B3, NM_001291952, Q02153, Q86WY5, uc011cip.1, uc011cip.2, uc011cip.3
UCSC ID: ENST00000505764.5
RefSeq Accession: NM_001291952
Protein: Q02153 (aka GCYB1_HUMAN)
CCDS: CCDS77977.1, CCDS47154.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.