Human Gene TLE4 (ENST00000265284.10) from GENCODE V44
  Description: Homo sapiens TLE family member 4, transcriptional corepressor (TLE4), transcript variant 17, mRNA. (from RefSeq NM_001351562)
Gencode Transcript: ENST00000265284.10
Gencode Gene: ENSG00000106829.21
Transcript (Including UTRs)
   Position: hg38 chr9:79,572,745-79,725,233 Size: 152,489 Total Exon Count: 19 Strand: +
Coding Region
   Position: hg38 chr9:79,572,791-79,725,144 Size: 152,354 Coding Exon Count: 19 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr9:79,572,745-79,725,233)mRNA (may differ from genome)Protein (748 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
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PubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TLE4_HUMAN
DESCRIPTION: RecName: Full=Transducin-like enhancer protein 4;
FUNCTION: Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity).
SUBUNIT: Homooligomer and heterooligomer with other family members. Binds PAX5, LEF1, TCF7, TCF7L1 and TCF7L2. Interacts with ZNF703; TLE4 may mediate ZNF703 transcriptional repression (By similarity).
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: In all tissues examined, mostly in brain, and muscle.
PTM: Phosphorylated. PAX5 binding increases phosphorylation (By similarity).
PTM: Ubiquitinated by XIAP/BIRC4.
SIMILARITY: Belongs to the WD repeat Groucho/TLE family.
SIMILARITY: Contains 7 WD repeats.
SEQUENCE CAUTION: Sequence=BAA86575.1; Type=Erroneous initiation; Sequence=CAC22598.1; Type=Erroneous gene model prediction; Sequence=CAC22599.1; Type=Erroneous gene model prediction; Sequence=CAI13644.1; Type=Erroneous gene model prediction; Sequence=CAI13734.1; Type=Erroneous gene model prediction; Sequence=CAI41315.1; Type=Erroneous gene model prediction;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 46.53 RPKM in Testis
Total median expression: 313.38 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -13.2046-0.287 Picture PostScript Text
3' UTR -10.6089-0.119 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR005617 - Groucho/TLE_N
IPR009146 - Groucho_enhance
IPR015943 - WD40/YVTN_repeat-like_dom
IPR001680 - WD40_repeat
IPR019775 - WD40_repeat_CS
IPR017986 - WD40_repeat_dom

Pfam Domains:
PF03920 - Groucho/TLE N-terminal Q-rich domain
PF00400 - WD domain, G-beta repeat

ModBase Predicted Comparative 3D Structure on Q04727
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003674 molecular_function
GO:0003682 chromatin binding
GO:0003705 transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
GO:0003714 transcription corepressor activity
GO:0005515 protein binding
GO:0070491 repressing transcription factor binding

Biological Process:
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0008150 biological_process
GO:0016055 Wnt signaling pathway
GO:0045892 negative regulation of transcription, DNA-templated
GO:1904837 beta-catenin-TCF complex assembly
GO:1990830 cellular response to leukemia inhibitory factor

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:1990907 beta-catenin-TCF complex


-  Descriptions from all associated GenBank mRNAs
  BC045650 - Homo sapiens transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila), mRNA (cDNA clone MGC:43541 IMAGE:5268902), complete cds.
BC036369 - Homo sapiens transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila), mRNA (cDNA clone IMAGE:4824600).
BC059405 - Homo sapiens transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila), mRNA (cDNA clone MGC:71782 IMAGE:5296117), complete cds.
AK057236 - Homo sapiens cDNA FLJ32674 fis, clone TESTI1000142, highly similar to TRANSDUCIN-LIKE ENHANCER PROTEIN 4.
AB033087 - Homo sapiens KIAA1261 mRNA for KIAA1261 protein.
AK302709 - Homo sapiens cDNA FLJ61488 complete cds, highly similar to Transducin-like enhancer protein 4.
AK296342 - Homo sapiens cDNA FLJ58005 complete cds, highly similar to Transducin-like enhancer protein 4.
AB463316 - Synthetic construct DNA, clone: pF1KA1261, Homo sapiens TLE4 gene for transducin-like enhancer of split 4, without stop codon, in Flexi system.
KJ901798 - Synthetic construct Homo sapiens clone ccsbBroadEn_11192 TLE4 gene, encodes complete protein.
AL162059 - Homo sapiens mRNA; cDNA DKFZp547P103 (from clone DKFZp547P103).
HQ283388 - Homo sapiens clone TLE4_E8_E5 TLE4 mRNA, partial sequence.
JD118878 - Sequence 99902 from Patent EP1572962.
JD322228 - Sequence 303252 from Patent EP1572962.
JD408679 - Sequence 389703 from Patent EP1572962.
M99439 - Human transducin-like enhancer protein (TLE4) mRNA, 3' end.
JD541438 - Sequence 522462 from Patent EP1572962.
JD251378 - Sequence 232402 from Patent EP1572962.
JD184949 - Sequence 165973 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q04727 (Reactome details) participates in the following event(s):

R-HSA-1912359 HES1 binds TLE
R-NUL-2065510 rHes1 binds TLE
R-HSA-4641229 TLE oligomerizes
R-HSA-4649028 TLE forms non-functional complexes with AES
R-HSA-3322431 XIAP binds TLE
R-HSA-8944349 TLE tetramers bind TCF/LEF at WNT promoters
R-HSA-3299569 Beta-catenin displaces TLE:HDAC1 from TCF/LEF
R-HSA-3322434 XIAP dissociates from ub-TLE
R-HSA-3322429 XIAP monoubiquinates TLE
R-HSA-4641231 TLE recruits HDAC1 to WNT promoters
R-HSA-2122947 NOTCH1 Intracellular Domain Regulates Transcription
R-HSA-4641265 Repression of WNT target genes
R-HSA-3769402 Deactivation of the beta-catenin transactivating complex
R-HSA-201722 Formation of the beta-catenin:TCF transactivating complex
R-HSA-1980143 Signaling by NOTCH1
R-HSA-195253 Degradation of beta-catenin by the destruction complex
R-HSA-201681 TCF dependent signaling in response to WNT
R-HSA-157118 Signaling by NOTCH
R-HSA-195721 Signaling by WNT
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000265284.1, ENST00000265284.2, ENST00000265284.3, ENST00000265284.4, ENST00000265284.5, ENST00000265284.6, ENST00000265284.7, ENST00000265284.8, ENST00000265284.9, KIAA1261, NM_001351562, Q04727, Q3ZCS1, Q5T1Y2, Q6PCB3, Q9BZ07, Q9BZ08, Q9BZ09, Q9NSL3, Q9ULF9, TLE4_HUMAN, uc011lsq.1, uc011lsq.2, uc011lsq.3, uc011lsq.4
UCSC ID: ENST00000265284.10
RefSeq Accession: NM_007005
Protein: Q04727 (aka TLE4_HUMAN)
CCDS: CCDS43837.1, CCDS65070.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.