Mouse Gene Nab2 (ENSMUST00000026469.8) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus Ngfi-A binding protein 2 (Nab2), transcript variant 1, mRNA. (from RefSeq NM_008668)
Gencode Transcript: ENSMUST00000026469.8
Gencode Gene: ENSMUSG00000025402.12
Transcript (Including UTRs)
   Position: mm10 chr10:127,660,918-127,666,710 Size: 5,793 Total Exon Count: 7 Strand: -
Coding Region
   Position: mm10 chr10:127,661,670-127,666,458 Size: 4,789 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther NamesModel Information
Methods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr10:127,660,918-127,666,710)mRNA (may differ from genome)Protein (525 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: NAB2_MOUSE
DESCRIPTION: RecName: Full=NGFI-A-binding protein 2; AltName: Full=EGR-1-binding protein 2;
FUNCTION: Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability.
SUBUNIT: Homomultimers may associate with EGR1 bound to DNA (By similarity).
SUBCELLULAR LOCATION: Nucleus. Note=Isoform 2 is not localized to the nucleus.
TISSUE SPECIFICITY: Highly expressed in brain and thymus, and at lower levels in spleen, kidney, heart and testis. Isoform 1 is predominantly expressed in testis, whereas isoform 3 is more abundant in thymus.
INDUCTION: By serum stimulation.
DOMAIN: The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
DOMAIN: The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.
PTM: Sumoylation by EGR2 represses EGR2 transcriptional activity in hindbrain (By similarity).
SIMILARITY: Belongs to the NAB family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -118.40252-0.470 Picture PostScript Text
3' UTR -246.12752-0.327 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR006989 - NAB_co-repressor_dom
IPR006988 - Nab_N

Pfam Domains:
PF04904 - NAB conserved region 1 (NCD1)
PF04905 - NAB conserved region 2 (NCD2)

SCOP Domains:
47769 - SAM/Pointed domain

ModBase Predicted Comparative 3D Structure on Q61127
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
GenBankRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0008134 transcription factor binding
GO:0042802 identical protein binding

Biological Process:
GO:0001958 endochondral ossification
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0014037 Schwann cell differentiation
GO:0016480 negative regulation of transcription from RNA polymerase III promoter
GO:0042552 myelination
GO:0045682 regulation of epidermis development
GO:0045892 negative regulation of transcription, DNA-templated
GO:1902949 positive regulation of tau-protein kinase activity

Cellular Component:
GO:0005634 nucleus


-  Descriptions from all associated GenBank mRNAs
  LF198303 - JP 2014500723-A/5806: Polycomb-Associated Non-Coding RNAs.
BC045139 - Mus musculus Ngfi-A binding protein 2, mRNA (cDNA clone MGC:54734 IMAGE:6306757), complete cds.
AK158688 - Mus musculus visual cortex cDNA, RIKEN full-length enriched library, clone:K230054E11 product:Ngfi-A binding protein 2, full insert sequence.
U47543 - Mus musculus NGFI-A binding protein 2 (NAB2) mRNA, complete cds.
AK171180 - Mus musculus NOD-derived CD11c +ve dendritic cells cDNA, RIKEN full-length enriched library, clone:F630301C09 product:Ngfi-A binding protein 2, full insert sequence.
AK155501 - Mus musculus NOD-derived CD11c +ve dendritic cells cDNA, RIKEN full-length enriched library, clone:F630308G08 product:Ngfi-A binding protein 2, full insert sequence.
LF198304 - JP 2014500723-A/5807: Polycomb-Associated Non-Coding RNAs.
MA433880 - JP 2018138019-A/5806: Polycomb-Associated Non-Coding RNAs.
MA433881 - JP 2018138019-A/5807: Polycomb-Associated Non-Coding RNAs.
AK034709 - Mus musculus 12 days embryo embryonic body between diaphragm region and neck cDNA, RIKEN full-length enriched library, clone:9430027D14 product:Ngfi-A binding protein 2, full insert sequence.
LF225392 - JP 2014500723-A/32895: Polycomb-Associated Non-Coding RNAs.
LF202989 - JP 2014500723-A/10492: Polycomb-Associated Non-Coding RNAs.
LF203859 - JP 2014500723-A/11362: Polycomb-Associated Non-Coding RNAs.
MA460969 - JP 2018138019-A/32895: Polycomb-Associated Non-Coding RNAs.
MA438566 - JP 2018138019-A/10492: Polycomb-Associated Non-Coding RNAs.
MA439436 - JP 2018138019-A/11362: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: NAB2_MOUSE, NM_008668, Q61127, Q80VR9, uc011xpt.1, uc011xpt.2
UCSC ID: uc011xpt.2
RefSeq Accession: NM_008668
Protein: Q61127 (aka NAB2_MOUSE)
CCDS: CCDS24247.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.