Mouse Gene L2hgdh (ENSMUST00000021370.9) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus L-2-hydroxyglutarate dehydrogenase (L2hgdh), mRNA. (from RefSeq NM_145443)
Gencode Transcript: ENSMUST00000021370.9
Gencode Gene: ENSMUSG00000020988.9
Transcript (Including UTRs)
   Position: mm10 chr12:69,690,433-69,724,873 Size: 34,441 Total Exon Count: 10 Strand: -
Coding Region
   Position: mm10 chr12:69,692,301-69,724,796 Size: 32,496 Coding Exon Count: 10 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr12:69,690,433-69,724,873)mRNA (may differ from genome)Protein (464 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: L2HDH_MOUSE
DESCRIPTION: RecName: Full=L-2-hydroxyglutarate dehydrogenase, mitochondrial; EC=1.1.99.2; AltName: Full=Duranin; Flags: Precursor;
CATALYTIC ACTIVITY: (S)-2-hydroxyglutarate + acceptor = 2- oxoglutarate + reduced acceptor.
COFACTOR: FAD (By similarity).
SUBCELLULAR LOCATION: Mitochondrion (By similarity).
SIMILARITY: Belongs to the L2HGDH family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -30.5077-0.396 Picture PostScript Text
3' UTR -511.011868-0.274 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR006076 - FAD-dep_OxRdtase

Pfam Domains:
PF01266 - FAD dependent oxidoreductase

SCOP Domains:
51735 - NAD(P)-binding Rossmann-fold domains
51905 - FAD/NAD(P)-binding domain
51971 - Nucleotide-binding domain
51984 - MurCD N-terminal domain

ModBase Predicted Comparative 3D Structure on Q91YP0
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologGenome BrowserNo orthologNo ortholog
Gene Details     
Gene Sorter     
GenBankRGDEnsemblEnsemblWormBase 
Protein SequenceProtein Sequence Protein Sequence  
AlignmentAlignment Alignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0016491 oxidoreductase activity
GO:0047545 2-hydroxyglutarate dehydrogenase activity

Biological Process:
GO:0044267 cellular protein metabolic process
GO:0055114 oxidation-reduction process

Cellular Component:
GO:0005739 mitochondrion
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  AK139539 - Mus musculus 2 cells egg cDNA, RIKEN full-length enriched library, clone:B020003L13 product:unclassifiable, full insert sequence.
AK145228 - Mus musculus mammary gland RCB-0527 Jyg-MC(B) cDNA, RIKEN full-length enriched library, clone:G930024M08 product:hypothetical FAD dependent oxidoreductase containing protein, full insert sequence.
AK168429 - Mus musculus 17 days pregnant adult female amnion cDNA, RIKEN full-length enriched library, clone:I920016C01 product:hypothetical FAD dependent oxidoreductase containing protein, full insert sequence.
BC016226 - Mus musculus L-2-hydroxyglutarate dehydrogenase, mRNA (cDNA clone MGC:28775 IMAGE:4487011), complete cds.
AK152450 - Mus musculus bone marrow macrophage cDNA, RIKEN full-length enriched library, clone:I830072L16 product:hypothetical FAD dependent oxidoreductase containing protein, full insert sequence.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu00650 - Butanoate metabolism

Reactome (by CSHL, EBI, and GO)

Protein Q91YP0 (Reactome details) participates in the following event(s):

R-MMU-880050 (S)-2-hydroxyglutarate + FAD => 2-oxoglutarate + FADH2
R-MMU-880009 Interconversion of 2-oxoglutarate and 2-hydroxyglutarate
R-MMU-71406 Pyruvate metabolism and Citric Acid (TCA) cycle
R-MMU-1428517 The citric acid (TCA) cycle and respiratory electron transport
R-MMU-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: L2HDH_MOUSE, NM_145443, Q3TH61, Q3U7Z0, Q3ULY6, Q91YP0, uc011ynb.1, uc011ynb.2
UCSC ID: uc011ynb.2
RefSeq Accession: NM_145443
Protein: Q91YP0 (aka L2HDH_MOUSE)
CCDS: CCDS25953.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.