Mouse Gene Amer2 (ENSMUST00000022561.8) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Negative regulator of the canonical Wnt signaling pathway involved in neuroectodermal patterning. Acts by specifically binding phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), translocating to the cell membrane and interacting with key regulators of the canonical Wnt signaling pathway, such as components of the beta-catenin destruction complex (By similarity). (from UniProt Q8CCJ4)
Gencode Transcript: ENSMUST00000022561.8
Gencode Gene: ENSMUSG00000021986.9
Transcript (Including UTRs)
   Position: mm10 chr14:60,377,692-60,388,197 Size: 10,506 Total Exon Count: 1 Strand: +
Coding Region
   Position: mm10 chr14:60,378,247-60,380,265 Size: 2,019 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther NamesModel Information
Methods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr14:60,377,692-60,388,197)mRNA (may differ from genome)Protein (672 aa)
Gene SorterGenome BrowserOther Species FASTATable SchemaAlphaFoldBioGPS
EnsemblExonPrimerGeneCardsMGIPubMedSOURCE
UniProtKB

-  Comments and Description Text from UniProtKB
  ID: AMER2_MOUSE
DESCRIPTION: RecName: Full=APC membrane recruitment protein 2; Short=Amer2; AltName: Full=Protein FAM123A;
FUNCTION: Negative regulator of the canonical Wnt signaling pathway involved in neuroectodermal patterning. Acts by specifically binding phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), translocating to the cell membrane and interacting with key regulators of the canonical Wnt signaling pathway, such as components of the beta-catenin destruction complex (By similarity).
SUBUNIT: Interacts with APC (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein (By similarity). Note=Translocates to the cell membrane following binding to PtdIns(4,5)P2 (By similarity).
SIMILARITY: Belongs to the Amer family.
SEQUENCE CAUTION: Sequence=AAH30356.1; Type=Erroneous initiation; Sequence=AAH56350.1; Type=Erroneous initiation; Sequence=BAB27452.1; Type=Erroneous initiation; Sequence=BAC27972.1; Type=Erroneous initiation; Sequence=BAE23912.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -172.00555-0.310 Picture PostScript Text
3' UTR -2185.007932-0.275 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR019003 - Uncharacterised_FAM123

Pfam Domains:
PF09422 - WTX protein

ModBase Predicted Comparative 3D Structure on Q8CCJ4
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
      
      
 RGDEnsembl   
 Protein Sequence    
 Alignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0005546 phosphatidylinositol-4,5-bisphosphate binding
GO:0008013 beta-catenin binding
GO:0008289 lipid binding

Biological Process:
GO:0007398 ectoderm development
GO:0016055 Wnt signaling pathway
GO:0048856 anatomical structure development
GO:0060828 regulation of canonical Wnt signaling pathway
GO:0090090 negative regulation of canonical Wnt signaling pathway

Cellular Component:
GO:0005886 plasma membrane
GO:0016020 membrane


-  Descriptions from all associated GenBank mRNAs
  LF200002 - JP 2014500723-A/7505: Polycomb-Associated Non-Coding RNAs.
LF204006 - JP 2014500723-A/11509: Polycomb-Associated Non-Coding RNAs.
LF203208 - JP 2014500723-A/10711: Polycomb-Associated Non-Coding RNAs.
BC056350 - Mus musculus family with sequence similarity 123, member A, mRNA (cDNA clone MGC:73465 IMAGE:6415605), complete cds.
AK139177 - Mus musculus 7 days neonate cerebellum cDNA, RIKEN full-length enriched library, clone:A730059D15 product:hypothetical protein, full insert sequence.
AK032651 - Mus musculus 10 days neonate cerebellum cDNA, RIKEN full-length enriched library, clone:6530402L19 product:hypothetical protein, full insert sequence.
AK011185 - Mus musculus 10 days embryo whole body cDNA, RIKEN full-length enriched library, clone:2600011E07 product:hypothetical protein, full insert sequence.
LF241261 - JP 2014500723-A/48764: Polycomb-Associated Non-Coding RNAs.
LF241262 - JP 2014500723-A/48765: Polycomb-Associated Non-Coding RNAs.
LF204008 - JP 2014500723-A/11511: Polycomb-Associated Non-Coding RNAs.
BC030356 - Mus musculus family with sequence similarity 123, member A, mRNA (cDNA clone IMAGE:5367534), partial cds.
AK012876 - Mus musculus 10, 11 days embryo whole body cDNA, RIKEN full-length enriched library, clone:2810037O22 product:unclassifiable, full insert sequence.
MA435579 - JP 2018138019-A/7505: Polycomb-Associated Non-Coding RNAs.
MA439583 - JP 2018138019-A/11509: Polycomb-Associated Non-Coding RNAs.
MA438785 - JP 2018138019-A/10711: Polycomb-Associated Non-Coding RNAs.
MA476838 - JP 2018138019-A/48764: Polycomb-Associated Non-Coding RNAs.
MA476839 - JP 2018138019-A/48765: Polycomb-Associated Non-Coding RNAs.
MA439585 - JP 2018138019-A/11511: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: AK139177, AMER2_MOUSE, Fam123a, Q7TNC5, Q8CCJ4, Q8K0U9, Q9D0Q2, uc011zmz.1, uc011zmz.2, uc011zmz.3
UCSC ID: uc011zmz.3
RefSeq Accession: NM_028113
Protein: Q8CCJ4 (aka AMER2_MOUSE)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.