Mouse Gene Add2 (ENSMUST00000203279.1) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. Calmodulin binds preferentially to the beta subunit (By similarity). (from UniProt Q9QYB8)
RefSeq Summary (NM_001271859): This gene encodes the beta subunit of the adducin family. Adducins, encoded by alpha, beta and gamma genes, are heteromeric proteins that crosslink actin filaments with spectrin at the cytoskeletal membrane. This protein, primarily found in the brain and hematopoietic cells, is regulated by phosphorylation and calmodulin interactions as it promotes spectrin assembly onto actin filaments, bundles actin and caps barbed ends of actin filaments. In mouse, deficiency of this gene can lead to mild hemolytic anemia and impaired synaptic plasticity. Mutations of this gene in mouse serve as a pathophysiological model for hereditary spherocytosis and hereditary elliptocytosis. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Dec 2012].
Gencode Transcript: ENSMUST00000203279.1
Gencode Gene: ENSMUSG00000030000.10
Transcript (Including UTRs)
   Position: mm10 chr6:86,085,862-86,118,801 Size: 32,940 Total Exon Count: 9 Strand: +
Coding Region
   Position: mm10 chr6:86,085,862-86,118,781 Size: 32,920 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr6:86,085,862-86,118,801)mRNA (may differ from genome)Protein (477 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblExonPrimerGeneCardsMGIPubMed
ReactomeSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: ADDB_MOUSE
DESCRIPTION: RecName: Full=Beta-adducin; AltName: Full=Add97; AltName: Full=Erythrocyte adducin subunit beta;
FUNCTION: Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. Calmodulin binds preferentially to the beta subunit (By similarity).
SUBUNIT: Heterodimer of an alpha and a beta subunit.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton (By similarity). Cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity).
DOMAIN: Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
SIMILARITY: Belongs to the aldolase class II family. Adducin subfamily.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
3' UTR -3.5020-0.175 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001303 - Aldolase_II/adducin_N

Pfam Domains:
PF00596 - Class II Aldolase and Adducin N-terminal domain

SCOP Domains:
53639 - AraD/HMP-PK domain-like

ModBase Predicted Comparative 3D Structure on Q9QYB8
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
GenBankRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003779 actin binding
GO:0005198 structural molecule activity
GO:0005516 calmodulin binding
GO:0019901 protein kinase binding
GO:0030507 spectrin binding
GO:0042803 protein homodimerization activity
GO:0046982 protein heterodimerization activity
GO:0051015 actin filament binding

Biological Process:
GO:0006811 ion transport
GO:0030097 hemopoiesis
GO:0032092 positive regulation of protein binding
GO:0050900 leukocyte migration
GO:0050901 leukocyte tethering or rolling
GO:0051016 barbed-end actin filament capping
GO:0051017 actin filament bundle assembly
GO:0065003 macromolecular complex assembly

Cellular Component:
GO:0005737 cytoplasm
GO:0005856 cytoskeleton
GO:0005886 plasma membrane
GO:0008290 F-actin capping protein complex
GO:0014069 postsynaptic density
GO:0016020 membrane
GO:0031410 cytoplasmic vesicle
GO:0044853 plasma membrane raft


-  Descriptions from all associated GenBank mRNAs
  AK029488 - Mus musculus 0 day neonate head cDNA, RIKEN full-length enriched library, clone:4833447N11 product:adducin 2 (beta), full insert sequence.
AK147544 - Mus musculus cDNA, RIKEN full-length enriched library, clone:M5C1070K15 product:adducin 2 (beta), full insert sequence.
AK156954 - Mus musculus activated spleen cDNA, RIKEN full-length enriched library, clone:F830109J20 product:adducin 2 (beta), full insert sequence.
AK014496 - Mus musculus 14 days embryo liver cDNA, RIKEN full-length enriched library, clone:4432414K09 product:adducin 2 (beta), full insert sequence.
AF100422 - Mus musculus beta-1 adducin (ADD2) mRNA, alternatively spliced, complete cds.
AF189769 - Mus musculus beta adducin mRNA, complete cds.
AF189770 - Mus musculus beta adducin delta mRNA, complete cds.
AK160736 - Mus musculus 13 days embryo head cDNA, RIKEN full-length enriched library, clone:3100003N03 product:adducin 2 (beta), full insert sequence.
BC046783 - Mus musculus adducin 2 (beta), mRNA (cDNA clone MGC:61263 IMAGE:6401200), complete cds.
BC053032 - Mus musculus adducin 2 (beta), mRNA (cDNA clone MGC:62261 IMAGE:6401200), complete cds.
AK028425 - Mus musculus 14 days embryo liver cDNA, RIKEN full-length enriched library, clone:4432405I01 product:adducin 2 (beta), full insert sequence.
AF100423 - Mus musculus beta-2 adducin (ADD2) mRNA, alternatively spliced, complete cds.
AB350415 - Mus musculus non-coding RNA, oocyte_piRNA1231, complete sequence.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9QYB8 (Reactome details) participates in the following event(s):

R-MMU-5226979 ADD1:ADD2 binds DMTN
R-MMU-5223345 Miscellaneous transport and binding events
R-MMU-382551 Transport of small molecules

-  Other Names for This Gene
  Alternate Gene Symbols: ADDB_MOUSE, AF189770, Q3U0E1, Q80VH9, Q8C1C4, Q9CXE3, Q9JLE4, Q9JLE5, Q9QYB7, Q9QYB8, uc012eoj.1, uc012eoj.2, uc012eoj.3
UCSC ID: uc012eoj.3
RefSeq Accession: NM_001271859
Protein: Q9QYB8 (aka ADDB_MOUSE)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.